Tri Harjaka
Department Of Crop Protection, Faculty Of Agriculture, Universitas Gadjah Mada Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281

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Detection of Cocoa Pod Borer Infestation Using Sex Pheromone Trap and its Control by Pod Wrapping Dian Rahmawati; Fransiscus Xaverius Wagiman; Tri Harjaka; Nugroho Susetya Putra
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 1 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (27.916 KB) | DOI: 10.22146/jpti.22659

Abstract

Cocoa pod borer (CPB), Conopomorpha cramerella Snellen (Lepidoptera: Gracillariidae) is a major pest of cocoa. Detection of the pest infestation using sex pheromone traps in the early growth and development of cocoa pods is important for an early warning system programme. In order to prevent the pest infestation the young pods were wrapped with plastic bags. A research to study the CPB incidence was conducted at cocoa plantations in Banjarharjo and Banjaroya villages, District of Kalibawang; Hargotirto and Hargowilis villages, District of Kokap; and Pagerharjo village, District of Samigaluh, Yogyakarta. The experiments design used RCBD with four treatments (sex pheromone trap, combination of sex pheromone trap and pod wrapping, pod wrapping, and control) and five replications. As many as 6 units/ha pheromone traps were installed with a distance of 40 m in between. Results showed that one month prior to the trap installation in the experimental plots there were ripen cocoa pods as many as 9-13%, which were mostly infested by CPB. During the time period of introducting research on August to Desember 2016 there was not rambutan fruits as the CPB host, hence the CPB resource was from infested cocoa pods. The CPB moth trapped as many as 0−7 (1.13 ± 0.14) moths/6 traps/12 observations. The seed damage due to CPB larvae in the pheromone trap treatments (23.98%) was relatively similar with the control (20.25%). Seed damage rate in combination treatment of pheromone trap and pod wrapping (0.59%) was relatively the same with the pod wrapping (0.20%). The pheromone trap was more usefull for monitoring tool rather than for control, meanwhile pod wrapping was an effective control measure of CPB. IntisariPenggerek Buah Kakao (PBK), Conopomorpha cramerella Snellen (Lepidoptera: Gracillariidae) merupakan salah satu hama utama kakao. Deteksi serangan hama PBK dengan perangkap feromon seks pada awal pertumbuhan dan perkembangan buah kakao penting dilakukan sebagai sistem peringatan dini. Dalam upaya pencegahan serangan hama tersebut dilakukan pembrongsongan buah muda. Penelitian dilakukan di kebun kakao rakyat di Desa Banjarharjo dan Banjaroya, Kecamatan Kalibawang, Desa Hargotirto dan Hargowilis, Kecamatan Kokap, serta kebun PT Pagilaran di Desa Pagerharjo, Kecamatan Samigaluh, Yogyakarta. Percobaan dengan empat perlakuan (perangkap feromon, kombinasi perangkap feromon dan brongsong, brongsong, kontrol) dan lima ulangan menggunakan rancangan RCBD. Perangkap feromon sebanyak 6 unit/ha dipasang dengan jarak antar perangkap sekitar 40 m. Hasil percobaan menunjukkan bahwa satu bulan sebelum pemasangan perangkap terdapat buah tua sebanyak 9−13 % dan umumnya terserang PBK. Selama kajian berlangsung tidak terdapat buah rambutan sebagai inang PBK sehingga sumber PBK diduga hanya dari buah kakao yang terserang. Ngengat PBK yang tertangkap yaitu sebanyak 0−7 (1,13±0,14) ekor/6 perangkap/12 pengamatan. Intensitas kerusakan biji akibat ulat PBK pada perlakuan perangkap feromon (23,98%) relatif sama dengan kontrol (20,25%). Kerusakan biji kakao pada perlakuan kombinasi perangkap feromon dan pembrongsongan (0,59%) relatif sama dengan pembrongsongan saja (0,20%). Perangkap feromon lebih berfungsi sebagai alat monitoring daripada alat pengendalian PBK, sedangkan pembrongsongan buah merupakan teknologi pengendalian PBK yang efektif.
Impact of Abamectin on Anagrus nilaparvatae, An Egg Parasitoid of Nilaparvata lugens Edi Eko Sasmito; Y. Andi Trisyono; Tri Harjaka
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.24759

Abstract

Anagrus nilaparvatae (Hymenoptera: Mymaridae) is an egg parasitoid potential for controlling the major pests on rice, the brown planthopper (Nilaparvata lugens [Hemiptera: Delphacidae]).Abamectin is one of insecticides registered for N. lugens. The research was aimed to investigate the impact of contact application of abamectin on the parasitism level of A. nilaparvatae under laboratory conditions. Adults of A. nilaparvatae and the first instars as well as adults of N. lugens were exposed to the residue of abamection inside the test tube. A. nilaparvatae was much more susceptible to abamectin compared to N. lugens. Application of abamectin at the recommended concentration (22.78 ppm) for 30 min caused 100% mortality, and it reduced to 85% when the concentration was decreased to 0.36 ppm. In contrast, the mortality for the first instar of N. lugens was only 15% at 22.78 and no mortality at 0.36 ppm. No N. lugens adults died even when they were exposed to 22.78 ppm. Furthermore, the parasitism test was conducted using 38 days after planting of IR-64 rice variety. Those plants were infested with 50 females of N. lugens for 2 days. A. nilaparvatae were exposed by contact to 0.02, 0.23, and 2.28 ppm of abamectin. The survivors were released to the rice plant containing eggs of N. lugens. Contact application of abamectin reduced parasitism level of A. nilaparvatae as much as 86.34, 70.01, and 28.43% with concentrations of 2.28 ppm, 0.23 and 0.02 ppm, respectively. In addition, the number of parasitoids emerged decreased with increasing concentration of abamectin. These results suggest that abamectin could be detrimental to A. nilaparvatae due to direct mortality, reduced the parasitism level, and decreased the number of progeny produced. IntisariAnagrus nilaparvatae (Hymenoptera: Mymaridae) merupakan salah satu parasitoid telur yang berpotensi untuk mengendalikan hama utama tanaman padi, wereng batang padi cokelat (Nilaparvata lugens [Hemiptera: Delphacidae]). Abamektin adalah salah satu insektisida yang terdaftar untuk pengendalian N. lugens. Penelitian ini bertujuan untuk mengetahui dampak aplikasi kontak abamektin terhadap suseptibilitas dan tingkat parasitasi A. nilaparvatae terhadap telur N. lugens pada kondisi laboratorium. Imago A. nilaparvatae serta instar satu dan imago N. lugens dipapar dengan residu abamektin di dalam tabung reaksi. A. nilaparvatae lebih peka terhadap abamektin dibandingkan N. lugens. Aplikasi abamektin pada konsentrasi anjuran (22,78 ppm) selama 30 menit menyebabkan mortalitas A. nilaparvatae 100%, dan mengurangi sampai dengan 85% pada konsentrasi yang lebih rendah 0,36 ppm. Sebaliknya, mortalitas instar satu N. lugens hanya sebesar 15% pada 22,78 ppm dan tidak menimbulkan kematian pada 0,36 ppm. Konsentrasi 22,78 ppm tidak menimbulkan kematian imago N. lugens. Selanjutnya, uji parasitasi dilakukan menggunakan media tanaman padi varietas IR-64 umur 38 hari setelah tanam. Tanaman diinfestasi dengan 50 ekor betina N. lugens selama dua hari. A. nilaparvatae dipapar abamektin dengan metode kontak pada konsentrasi 0.02, 0,23, dan 2,28 ppm. Parasitoid yang mampu bertahan hidup dilepaskan pada tanaman padi yang telah diinfestasi telur N. lugens. Aplikasi kontak abamektin mengurangi tingkat parasitasi A. nilaparvatae sebesar 86,34, 70.01, dan 28,43% pada konsentrasi 2,28; 0,23; dan 0,02 ppm. Selain itu, jumlah parasitoid yang muncul semakin menurun dengan peningkatan konsentrasi abamektin. Hasil ini menunjukkan bahwa abamektin dapat merugikan secara langsung terhadap mortalitas serta mengurangi tingkat parasitasi dan jumlah keturunan A. nilaparvatae.
Compatibility Between Beauveria bassiana (Bals.) and Neem Extract against Brown Plant-Hopper (Nilaparvata lugens Stal.) Nova Laili Wisuda; Tri Harjaka; Indah Dwi Prasetyaningrum
Jurnal Ilmu Pertanian Vol 4, No 1 (2019): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.43074

Abstract

Beauveria bassiana fungi and neem extract as botanical insecticide can be combined to get the synergizing effect to control brown planthopper (BPH). This study was conducted to determine the best combination between the spore density of B. bassiana and concentration of neem extract to control BPH effectively. The two factorial treatment consisted concentration of B. bassiana (106  Colony Forming Unit (CFU) and 107 CFU) and concentration of neem extract (5%, 10%, 15%), and control.  The treatments were assigned in Completely Randomized Design with three replications. The data were analyzed using ANOVA and  LSD at 5%. The test was conducted in vitro and bio assay of testing insects, BPH, was conducted using dipping method.  B. bassiana was suitable with neem extract with T value ranged from 78.58- 90.38. The mortality of BPH occurred on day 5 after application. The highest mortality (91.67%) occurred on the treatment with B. bassiana 107 CFU + neem extract 10%. The shortest LT50 was found at the treatment of B. bassiana 106 CFU+  neem extract 15%  (2.74 days) but not significantly different from B. bassiana 107 CFU + neem extract 15% (2.76 days). B. bassiana spores combined with neem extract are assumed to speed up the mortality of BPH by increasing the concentration.
Parasitization Levels of Spodoptera frugiperda Eggs (Smith) (Lepidoptera: Noctuidae) in Three Different Corn Ecosystems in East Java Rizki Dwi Wahyuningsih; Tri Harjaka; Suputa Suputa; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 26, No 1 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.71598

Abstract

Spodoptera frugiperda  (Lepidoptera: Noctuidae) has successfully invaded and spread to almost all provinces of Indonesia which may cause significant impacts on corn production. Local natural enemies could play an important role in managing this invasive insect, and more diverse ecosystems would benefit natural enemies. This study aimed to assess parasitization rates of S. frugiperda eggs by local egg parasitoids in three different corn ecosystems (agroforestry, rice field, and rainfed field) in East Java. Sentinel egg masses were used for this study by exposing eight-hour-old egg masses collected from the laboratory mass-rearing and left for 24 hours in corn plantations aged 7, 14, and 28 days after planting. Telenomus sp. was more abundant in the three ecosystems compared to Trichogramma sp. The egg mass parasitization varied from 15.6 to 52.5%. The number of egg masses parasitized was consistently higher in agroforestry, followed by rice fields and rainfed fields in all three different sampling times. Interestingly parasitization rates on egg masses were not different, and they ranged from 43.7 to 81.6%. These findings provide evidence on the importance of local egg parasitoids for managing S. frugiperda and some insights related to plant diversity to improving the services by these parasitoids. 
Biology and Demography of Helopeltis bradyi Waterhouse (Hemiptera: Miridae) Reared on Cucumbers Rich Gemilang Simanjuntak; Tri Harjaka; Arman Wijonarko
Jurnal Perlindungan Tanaman Indonesia Vol 26, No 1 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.71761

Abstract

Helopeltis bradyi is often used as for research and is required in large numbers. The insects were obtained from cacao plants and reared with cucumber fruit in the laboratory. This study aimed to determine the biology and demography of H. bradyi reared on cucumber under laboratory conditions. The demographic parameters observed were net reproduction rate (Ro), gross reproduction rate (GRR), intrinsic growth rate (r), average generation period (T), and population doubling (DT). The results showed that the egg stage lasted for 6.33 ± 0.47 days, the developmental period of the I‒V instar nymph was 2.13 ± 0.34, 2.07 ± 0.25, 2.13 ± 0.34, 2.33 ± 0.47, and 3.20 ± 0.40 days. The lifespan of male imagoes were 32.33 ± 4.92 days, and female imagoes were 24.60 ± 6.64 days. The adult female pre-oviposition period was 2.2 ± 0.40 days and oviposition period of 22.9 ± 4.66 days. H. bradyi has a type III survival curve which indicated high mortality rates during the first instar egg and nymph population. Demographic statistics of H. bradyi on cucumbers fruit are GRR of 296 individuals per generation, Ro of 196 individuals per parent per generation, rate of r of 0.18 individuals per parent per day, T of  29.34 days, and DT of  3.85 days.
The Potency of Metarhizium anisopliae in Disturbing Oryctes rhinoceros (Coleoptera: Scarabaeidae) Growth and Development Ummi Maysaroh; Edhi Martono; Tri Harjaka
Jurnal Perlindungan Tanaman Indonesia Vol 26, No 1 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.71755

Abstract

Metarhizium anisopliae is one of the most frequently used insect pathogen fungi in controlling Oryctes rhinoceros. This research aims to learn the potency of fungus M. anisopliae against O. rhinoceros larvae growth and development at the laboratory. The research methods used are T-tests comprising seven treatments and three replications. Fungi were applied in all larvae instar stages starting from the pre-molting of the first larvae instar, post-molting of the second larvae instar, active second larvae instar, pre-molting of the second larvae instar, post-molting of the third larvae instar, active third larvae instar, and pre-pupae stage which were then compared with each instar’s own control. The result indicates that fungus M. anisopliae is capable of suppressing O. rhinoceros growth and development. The fungus induced highest mortality rate of 87% to the third instar larvae and lowest mortality rate of 27% to the pre-molting of the first instar larvae. The fungus also affected the duration of larval stage. At pre-molting of the third larvae instar treated with M. anisopliae, the larval duration was 40 days compared to that of control that took 135 days. At the post-molting of the third larvae instar, the larval duration was 25 days compared to that of control that took 120 days. At the third larvae instar, the larval duration was no more than 15 days compared to that of control that reached 110 days. At pre-pupal stage, the larvae only lasted for 6 days while at control, they were able to last for 15 days. The fungus also affected the success of larva development in becoming pupae in all O. rhinoceros larval stage. The lowest success rate was found in the post-molting of the third larvae instar treated with M. anisopliae with 7% compared to its control with 100% while the highest success rate was found in the pre-molting of the first larval instar with 47% compared to its control with 93%.