Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Jurnal Peternakan Sriwijaya Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Dadang Mulyadi Saleh
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Pengaruh Interval Inseminasi Buatan dan Konsentrasi Spermatozoa Terhadap Daya Tunas dan Kematian Embrio Ayam Sentul Muhamad Nur Irfan; Sigit Mugiyono; Dadang Mulyadi Saleh
Jurnal Peternakan Sriwijaya Vol 10, No 2 (2021): JURNAL PETERNAKAN SRIWIJAYA
Publisher : Department of Animal Sciences, Faculty of Agriculture, Sriwijaya University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/JPS.10.2.2021.14446

Abstract

Penelitian ini dilakukan untuk mengetahui taraf optimal pengaruh interval inseminasi buatan (IB) dan konsentrasi spermatozoa terhadap daya tunas serta kematian embrio ayam Sentul. Penelitian dilaksanakan menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) faktorial pola (3x3) terdiri atas sembilan kombinasi perlakuan dan tiga ulangan. Materi penelitian yang digunakan 108 ekor ayam Sentul betina dan 24 ekor ayam Sentul jantan. Interval IB (I) sebagai faktor pertama terdiri atas i1 (setiap tiga hari), i2 (setiap enam hari), dan i3 (setiap sembilan hari). Konsentrasi spermatozoa (K) sebagai faktor kedua terdiri atas k1 (50 juta), k2 (100 juta), dan k3 (150 juta). Hasil analisis variansi menunjukkan bahwa interaksi antara interval IB dengan konsentrasi spermatozoa berpengaruh tidak nyata (P>0,05), interval IB berpengaruh sangat nyata (P<0,01), dan konsentrasi spermatozoa berpengaruh nyata (P<0,05) terhadap daya tunas ayam Sentul. Hasil uji lanjut, yaitu uji orthogonal polynomial menunjukkan titik optimal interval IB pada interval lima hari menghasilkan daya tunas 91,34% dengan persamaan kuadratik Y=62,381+11,542X–1,150X², dan titik optimal konsentrasi spermatozoa pada 109 juta menghasilkan daya tunas 90,51% dengan persamaan kuadratik Y=42,657+0,875X–0,004X². Hasil analisis variansi kematian embrio menunjukkan bahwa interval IB, konsentrasi spermatozoa, dan interaksi antara kedua faktor tersebut berpengaruh tidak nyata (P>0,05) terhadap kematian embrio ayam Sentul.
Motilitas dan Viabilitas Spermatozoa Itik Lokal (Anas platyrhyncos) Setelah Penyimpanan dalam Medium Berbeda Dikombinasi Krioprotektan Kuning Telur Berbagai Konsentrasi Yusni Atifah; Dadang Mulyadi Saleh; Hendro Pramono; Yulia Sistina
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.118

Abstract

The purpose of this study is to asses motility and viability of domestic duck (Anas platyrhyncos) spermatozoa after 6 day storing in preservation medium of different egg yolk cryoprotectant concentration in three different medium Tris, PBS or Ringer lactate. In this experiment, we applied 3 combination of factors as treatments : extender (Tris, PBS or Ringer lactate), egg yolk (EY) concentration (0, 5, 10, 15 or 25%) and storage time (0,1,2,3,4,5,6 days). Spermatozoa was obtained by massage technique, ejaculate then were swup up in the extender, the swup-up sperm then were incubated in preservation medium as each treatments subjected to motility and viability assesment before and after storage at refrigerator temperature (50C). The results showed that sperm motility and viability were highly significantly different (P<0.01) among 107 treatments. Extender factor as well as storage duration time were highly significantly different (P<0.01) affected the motility and viability of the treated sperm. EY concentration was significantly (P<0.05) affected viability, however there were no factor (P>0.05) affected motility of the sperm. The best treatment resulted in promising quality having the highest motility of 46,67 ± 32,15 5 motile was from the treatment of 10% EY in PBS stored for 4 days. The best viability result was from the tretament of 25% EY in PBS after 4 days storage resulted in 50,00 ± 36,05 % of viabie sperm. In conclusion this protocol could be applied for artificial insemination trial.
Motilitas dan Viabilitas Spermatozoa Itik Lokal (Anas platyrhynchos) Setelah Penyimpanan Refrigerator dalam Ekstender Dikombinasi Berbagai Konsentrasi Krioprotektan Gliserol Arie Amelia; Dadang Mulyadi Saleh; Hendro Pramono; Yulia Sistina
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.116

Abstract

The economic value of ducks has increased one Day Old Duck in large quantities and short time, effects on artificial insemination technologies improvement. Spermatozoa used for artificial insemination, could be derived from sperm preservation biotechnology, by means to maintain or extend their viability and motility of those spermatozoa. Extenders and cryoprotectant are needed for maintaining sperm motility and viability during storage or preservation. This study aims to assess motility and viability of local ducks spermatozoa after six days srotrage in 3 different extenders (Ringer's lactate, Tris or PBS) in combination with 5 different concentrations (0, 2, 4, 6, and 8%) of cryoprotectant glycerol. Results showed that sperm motility and viability was very highly significant (P<0.01) different among treatments, extender factor as well as duration storage time also very highly significantly (P<0.01) affected motility and viability of treated sperm. However, glycerol concentration factor was not significant (P>0.05) affected motility and viability of the sperm. The best treatment, resulted in the highest percentage motility and viability of sperm with progressivity score >1, was the 8% glycerol in PBS storage for up to 2 days (75.00 ± 21.79% and 71.67±14.43%). This protocol could be applied in artificial insemination trial.
Co-Authors Arie Amelia Atifah, Yusni Hendro Pramono Muhamad Nur Irfan Sigit Mugiyono Yulia Sistina