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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Yulia Sistina
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Deteksi Triploid Ikan Nilem (Osteochilus hasselti Valencienes 1842) Hasil Kejut Dingin 4oC Desi Susanti; Edy Yuwono; Yulia Sistina
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 17, No 3 (2012): October 2012
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v17i3.149

Abstract

Protokol triploidisasi cyprinidae nilem dengan kejut dingin 4oC pada 1, 3, atau 5 menit dari waktu fertilisasi dengan lama kejut 15, 20, atau 25 menit terbukti efektif dari parameyter fertilitas, daya tetas, abnormalitas larva, sintasan benih, panjang benih, dan khususnya data dimensi eritrosit benih hasil perlakuan. Dimensi eritrosit berupa ukuran panjang (major axes), lebar (minor axes), luas, dan volume eritrosit. Benih hasil perlakuan kejut dingin terbukti triploid dengan dimensi eritrositnya secara sangat nyata (P<0,01) jauh lebih besar dibanding diploid normal kontrolnya. Perlakuan kejut dingin secara sangat nyata (P<0,01) mempengaruhi fertilitas telur dan panjang tubuh benih, namun tidak nyata (P>0,05) mempengaruhi persentase penetasan, persentase abnormalitas larva, dan sintasan benih. Penerapan protokol triploid kejut dingin nilem yang terbukit efektif untuk pada bidang akuakultur siap dijalankan.Kata kunci: Deteksi triploid, kejut dingin, lama kejut, nilem, dimensi eritrosit
Motilitas dan Viabilitas Spermatozoa Itik Lokal (Anas platyrhyncos) Setelah Penyimpanan dalam Medium Berbeda Dikombinasi Krioprotektan Kuning Telur Berbagai Konsentrasi Yusni Atifah; Dadang Mulyadi Saleh; Hendro Pramono; Yulia Sistina
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.118

Abstract

The purpose of this study is to asses motility and viability of domestic duck (Anas platyrhyncos) spermatozoa after 6 day storing in preservation medium of different egg yolk cryoprotectant concentration in three different medium Tris, PBS or Ringer lactate. In this experiment, we applied 3 combination of factors as treatments : extender (Tris, PBS or Ringer lactate), egg yolk (EY) concentration (0, 5, 10, 15 or 25%) and storage time (0,1,2,3,4,5,6 days). Spermatozoa was obtained by massage technique, ejaculate then were swup up in the extender, the swup-up sperm then were incubated in preservation medium as each treatments subjected to motility and viability assesment before and after storage at refrigerator temperature (50C). The results showed that sperm motility and viability were highly significantly different (P<0.01) among 107 treatments. Extender factor as well as storage duration time were highly significantly different (P<0.01) affected the motility and viability of the treated sperm. EY concentration was significantly (P<0.05) affected viability, however there were no factor (P>0.05) affected motility of the sperm. The best treatment resulted in promising quality having the highest motility of 46,67 ± 32,15 5 motile was from the treatment of 10% EY in PBS stored for 4 days. The best viability result was from the tretament of 25% EY in PBS after 4 days storage resulted in 50,00 ± 36,05 % of viabie sperm. In conclusion this protocol could be applied for artificial insemination trial.
Motilitas dan Viabilitas Spermatozoa Itik Lokal (Anas platyrhynchos) Setelah Penyimpanan Refrigerator dalam Ekstender Dikombinasi Berbagai Konsentrasi Krioprotektan Gliserol Arie Amelia; Dadang Mulyadi Saleh; Hendro Pramono; Yulia Sistina
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.116

Abstract

The economic value of ducks has increased one Day Old Duck in large quantities and short time, effects on artificial insemination technologies improvement. Spermatozoa used for artificial insemination, could be derived from sperm preservation biotechnology, by means to maintain or extend their viability and motility of those spermatozoa. Extenders and cryoprotectant are needed for maintaining sperm motility and viability during storage or preservation. This study aims to assess motility and viability of local ducks spermatozoa after six days srotrage in 3 different extenders (Ringer's lactate, Tris or PBS) in combination with 5 different concentrations (0, 2, 4, 6, and 8%) of cryoprotectant glycerol. Results showed that sperm motility and viability was very highly significant (P<0.01) different among treatments, extender factor as well as duration storage time also very highly significantly (P<0.01) affected motility and viability of treated sperm. However, glycerol concentration factor was not significant (P>0.05) affected motility and viability of the sperm. The best treatment, resulted in the highest percentage motility and viability of sperm with progressivity score >1, was the 8% glycerol in PBS storage for up to 2 days (75.00 ± 21.79% and 71.67±14.43%). This protocol could be applied in artificial insemination trial.
Fekunditas Udang Galah (Macrobrachium rosenbergii ) Betina Pasca Ablasi Unilateral dan Suplementasi Vitamin E pada Pakan Rachmawati Nasution; Moh. Husein Sastranegara; Yulia Sistina
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 3 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.3.135

Abstract

This study reports on giant freshwater prawn fecundity with or without unilateral ablation treatment combined with alpha tocopherol (vit E) during 3 month culture. This exsperimental study applied 8 combination treatments of 4 different vit E dosages, i.e. 0, 200, 400, and 600 IU per kg feed with or without unilateral ablation. The results showed that treatments very highly significantly different (p<0,01) in fecundity with the 200 IU vit E dose combined with unilateral ablation was the best (45.228 ± 3.867 egg) optimum resulted in fecundity of the treated females. The overall fecundities averages were ablation group with 0 IU = 41.835 ± 837 eggs; 200 IU = 45.228 ± 3.867 eggs; 400 IU = 43394 ± 1523 eggs ; and the 600 IU = 43.718 ± 2.255 eggs, and for the non ablation with vit E group were 0 IU = 45.293 ± 2.899 eggs, 200 IU = 44.106 ± 1.759 eggs, 400 IU = 46.623 ± 2.556 eggs, and 600 IU=51.824 ± 1.132 eggs. The analysis results also proven that ablation factor gave very highly significantly (p<0,01) and vit E factor separately and in combination with ablation significantly (p<0,05) determined the females fecundity. In conclution, combination of unilateral ablation and tocopherol effective improved fecundity and the combination of unilateral ablation and vit E 200 IU the optimum resulted in giant freshwater prawn females fecundity.
Co-Authors Arie Amelia Atifah, Yusni Dadang Mulyadi Saleh Desi Susanti Edy Yuwono Hendro Pramono Moh. Husein Sastranegara Rachmawati Nasution