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Journal : METAMORFOSA Journal of Biological Sciences

Efek Toksik Minyak Atsiri Limbah Daun Kayu Manis (Cinnamomum burmanii (Nees & T.Nees) Blume.) dalam Mengendalikan Helopeltis antonii Signoret pada Tanaman Kakao secara in Vitro Roza Puspita; Resti Rahayu; Mairawita Mairawita; Nasril Nasir; Nurmansyah Nurmansyah
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p09

Abstract

The toxic effect of essential oil from waste cinnamon leaf (Cinnamomum burmanii) against pests of cocoa fruit Helopeltis antonii (Hemiptera; Miridae), has been done in Green House, Research Station Spice and Medicinal Plants, Laing Solok, West Sumatra in April to May 2016. The experiment aimed to determine repellant and lethal concentrations of essential oil from waste s cinnamon leaf (C. burmanii) for H. antonii. There were 5 treatments arranged in Complately Randomized Design with 4 replications. The treatments tested were essentialoil concentration f (0.05 ml/tube, 0.1 ml/tube, 0.2 ml/tube and 0.5 ml/tube) and control. The results showed that the effective repellent concentration were 0,05 ml/tube and 0,1 ml/tube which repelled H. antonii 0,03 % . The effecive lethal concentration was 0,05 ml/tube which killed H. antonii 57,50 %. Concentration used was highly toxic which killed l H. Antonii within six hours so of the repellent nature was notobserved.
Antagonis Pseudomonas fluorescens indegenous terhadap Ralstonia solanacearum pada Tanaman Tomat (Lycopersicum esculentum) Armaleni Armaleni; Nasril Nasir; Anthoni Agustien
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p19

Abstract

Tomato (L. esculentum) is one of the most important vegetable crops produce in West Sumatra. In this province, tomato are cultivated at highland regions of Solok, Padang Panjang, Tanah Datar and Bukittinggi. Annually, the increasing demand of this crop comes from other provinces in Sumatra and Java. This opportunity stimulate tomato’s agribusiness activity in West Sumatra. However, the crop succumb to the most destroyed bacterial wilt disease caused by Ralstonia solanacearum. The aims of this study to select antagonist Pseudomonas fluorescent as a biological control agent against R.solanacearum. The candidates of antagonist P. fluorescetn were collected from diseased tomato with R. solanacearum’s symptom in several tomato’s plantations in West Sumatra. Study of the antagonist was conducted from March to July 2016 at the Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences Andalas University. To find the antagonist work, each of the collected candidate was grown opposite R. solanacearum on Nutrient Agar medium. The result found that there were16 isolates of P. fluorescent occurred. The best antagonist ability amongst the candidates was from Pfi with diameter zone was 4,95 cm.Keywords: tomato, Ralstonia solanacearum, Pseudomonas fluorescens, biological control agent, Pfi