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All Journal Jurnal Hortikultura Indonesia (JHI) METAMORFOSA Journal of Biological Sciences
Ni Luh Putu Kayika Febryanti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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INDUKSI PERTUMBUHAN TUNAS DARI EKSPLAN ANGGREK Dendrobium Heterocarpum Lindl. DENGAN PEMBERIAN HORMON ZEATIN DAN NAA Ni Luh Putu Kayika Febryanti; Made Ria Defiani; Ida Ayu Astarini
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p07

Abstract

Dendrobium heterocarpum Lindl. is a native orchid in Indonesia. Its population continue to decline due to unsustainable exploitation. One solution to increase its population is via in vitro technique propagation. This research aimed to determine effect of hormone addition (Zeatin and NAA) to induce shoot multiplication of D. heterocarpum Lindl. grown in Murashige and Skoog (MS) basic medium. The research was conducted in Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Science, Udayana University from November 2015 until March 2016. The experiment employed Completely Randomized Design (CRD) with 20 combinations of treatments, with three replicates each treatment. The treatment werer addition of Zeatin and NAA, with five concentrations of Zeatin (0, 0.1, 0,5, sweet corn and hybrids corn as the natural Zeatin); four NAA concentrations (0, 0.05, 0.1 and 0.5). Variable recorded include such as the number of shoots, roots and explant’s vigor. The quantitative data which include number of leaves and shoots after 12 weeks, were analyzed using Analysis of Variance (ANOVA); and if significantly different, tested further using Duncan Multiple Range Test (DMRT) at 5% level. Z5N3 medium revealed significantly different on amount of leaves and shoot height and also the best medium on amount of roots. Best hormone combination that can induce multiplication of new shoots are medium Z4N3 and Z5N1.
Pertumbuhan Seedling Anggrek Tanah (Spathoglottis plicata Blume) in Vitro sebagai Respons terhadap Berbagai Komposisi Media MS: In Vitro Seedling Growth of Ground Orchid (Spathoglottis plicata Blume) as a Response to Various MS Media Compositions Ida Ayu Astarini; I Made Saka Wijaya; Ni Luh Putu Kayika Febryanti; Defiani, Made Ria
Jurnal Hortikultura Indonesia (JHI) Vol. 15 No. 1 (2024): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.15.1.49-54

Abstract

Anggrek tanah (Spathoglottis plicata Blume) adalah salah satu spesies anggrek tanah yang bernilai ekonomi dan estetika tinggi dan eksistensi di habitat aslinya termasuk katagori rawan (vulnerable). Keberadaan anggrek tanah perlu dipertahankan untuk menjaga sumber plasmanutfah anggrek yang dapat ditanam pada media tanah. Anggrek tanah dapat diperbanyak dengan pemisahan rumpun dan kultur biji melalui kultur jaringan. Penelitian bertujuan mempelajari perkembangan protokorm menjadi seedlings in vitro sebagai respons terhadap berbagai konsentrasi garam mineral dalam media MS. Protokorm anggrek berumur 6 MSS (minggu setelah sebar biji) ditanam secara aseptik di media MS dengan 4 variasi konsentrasi garam mineral makro dan mikronya (¼ MS, ½ MS, MS0 dan MS1 dengan tambahan NAA dan BAP). Percobaan ini dilaksanakan menggunakan rancangan acak lengkap (RAL) dengan 10 ulangan. Setiap satuan percobaan terdiri dari satu botol kultur, yang masing-masing berisi 5 clump protokorm. Pertumbuhan seedlings anggrek terbaik pada umur 6 bulan setelah tanam diperoleh pada media MS0, yang ditunjukkan oleh seedling dengan jumlah daun dan tunas terbanyak. Aklimatisasi planlet anggrek tanah juga dilakukan dengan menggunakan media arang sekam di rumah plastik bernaungan. Kata kunci: BAP, NAA, Aklimatisasi, Protocorm
Co-Authors I Made Saka Wijaya IDA AYU ASTARINI Made Ria Defiani