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HUBUNGAN ANTARA STATUS INVASI MIOMETRIUM DENGAN EKSPRESI CLAUDIN-4 DAN MATRIX METALLOPROTEINASE-2 PADA KARSINOMA ENDOMETRIOID ENDOMETRIUM Lestari, Neviana Fitri; Joewarini, Endang; Rahniayu, Alphania
Majalah Kesehatan FKUB Vol 6, No 1 (2019): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Â Â Karsinoma endometrium merupakan keganasan terbanyak kelima pada organ reproduksi wanita dengan jenis subtipe terbanyak adalah tipe endometrioid. Salah satu parameter penentuan stadium adalah kedalaman invasi miometrium yang menentukan peningkatan agresifitas dan progresifitas tumor yang berhubungan dengan luas tindakan operasi. Protein claudin-4 dan matrix metalloproteinase-2 (MMP-2) adalah protein penentu dalam invasi karsinoma. Penelitian ini bertujuan mengungkap peran claudin-4 dan MMP-2 dalam invasi myometrium karsinoma endometrioid endometrium. Penelitian ini merupakan penelitian analitik observasional dengan pendekatan cross sectional menggunakan sampel 44 blok parafin sampel karsinoma endometrioid endometrium di RSUD Dr. Soetomo periode 1 Januari-31 Desember 2017. Sampel dibagi menjadi kelompok status invasi kurang dan lebih dari separuh tebal miometrium. Pulasan imununohistokimia menggunakan antibodi monoklonal claudin-4 dan MMP-2, ekspresi dinilai berdasarkan nilai scoring semikuantitatif. Hubungan berbagai variabel dianalisis menggunakan tes korelasi Spearman rho. Hasil penelitian menunjukkan hubungan yang bermakna antara status invasi miometrium dengan ekspresi claudin-4 (p = 0,005). Tidak didapatkan hubungan yang bermakna antara status invasi miometrium dengan ekspresi MMP-2 (p = 0,549). Didapatkan hubungan yang bermakna antara ekspresi claudin-4 dengan ekspresi MMP-2 (p = 0,001). Kesimpulan dari penelitian ini adalah ekspresi claudin-4 berhubungan dengan status invasi miometrium karsinoma endometrioid endometrium, ekspresi MMP-2 tidak berhubungan dengan status invasi miometrium karsinoma endometrioid endometrium serta terdapat hubungan bermakna antara ekspresi claudin-4 dan MMP-2 pada karsinoma endometrioid endometrium.

KORELASI ANTARA STATUS METASTATIK KELENJAR GETAH BENING AKSILA DENGAN EKSPRESI CD44 DAN CXCR4 PADA KARSINOMA PAYUDARA Trianto, Heru Fajar; Joewarini, Endang; Rahniayu, Alphania
Majalah Kesehatan FKUB Vol 6, No 2 (2019): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Karsinoma payudara merupakan salah satu keganasan terbanyak yang menyerang wanita di dunia.Â Salah satu faktor prognosis karsinoma payudara adalah metastasis padaÂ kelenjar getah bening aksila. CD44 merupakan protein transmembran dan penanda sel punca kanker yang memiliki pengaruh pada pertumbuhan tumor, metastasis, dan rekurensi. CXCR4 merupakan reseptor kemokin yang terekspresi pada beberapa sel tumor salah satunya karsinoma payudara. Penelitian ini bertujuan membuktikan adanya korelasi antara status metastatik kelenjar getah bening aksila dengan ekspresi CD44 dan CXCR4 pada karsinoma payudara. Sebanyak 46 sampelÂ blok parafin pasien karsinoma payudara diperoleh dari Instalasi Patologi Anatomik RSUD Dr.Soetomo Surabaya periode Januari- Desember 2017. Blok parafin dipotong dan dilakukan pulasan immunohistokimia dengan antibodi monoklonal CD44 dan CXCR4. Ekspresi CD44 dan CXCR4 dinilai menggunakan skor imunoreaktif (Immunoreactive score (IRS)). Hasil menunjukkan ekspresi CD44 dan CXCR lebih kuat pada karsinoma payudara dengan metastasis kelenjar getah bening aksila dibandingkan tanpa metastasis kelenjar getah bening aksila (p = 0,001 danÂ p = 0,005). Terdapat korelasi positif yang signifikan antara status metastatik kelenjar getah bening aksila dengan ekspresi CD44 dan CXCR4 (p = 0,001 dan p = 0,004). Kesimpulannya adalah terdapat korelasi yang signifikan antara ekspresi CD44 dan CXCR4 dengan kejadian metastasis kelenjar getah bening pada karsinoma payudara. Hal ini menunjukkan bahwa CD44 dan CXCR4 Â memiliki peran penting dalam kejadian metastasis kelenjar getah bening pada karsinoma payudara.Â

INTRATESTICULAR INJECTION OF 20% HYPERTONIC SODIUM CHLORIDE AS A NOVEL CASTRATION METHOD: A PRECLINICAL STUDY Hendry, Marta; Hardjowijoto, Sunaryo; Purnomo, Basuki B; Joewarini, Endang; Meles, Dewa Ketut; P, Widodo J
Indonesian Journal of Urology Vol 17 No 1 (2010)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v17i1.339

Objectives: To demonstrate that intratesticular injection of 20% hypertonic sodium chloride can result in permanent castration and to evaluate serum changes in sodium chloride levels. Materials and Methods: A total of 40 Wistar rats were divided into 4 groups, consisting of bilateral orchidectomy (n=10), control (n=10) and 2 groups receiving intratesticular injections of 20% sodium chloride (n=10 in each group). Serum testosterone was measured on day 0, day 1, day 15 and day 30. Serum sodium chloride was assessed before injection, at 1 hour and 24 hours after injection. All testicles were harvested for histological examination. One way ANOVA and student t-test were used for statistical analysis. Results: Serum testosterone decreased to castrate levels in the orchidectomy and injected groups with no significant difference (p>0,05). Significant rise in serum sodium chloride was found 1 hour post injection (p<0,05) but after 1 day it decreased significantly (p<0,05). There was no significant difference in histopathological findings between the 2 injected groups after day 15 and 30 (p>0,05). Conclusion: Twenty percent hypertonic chloride injection has the same permanent castration effect with bilateral orchidectomy in rats. The serum sodium chloride changes did not reach the lethal level for rats. Therefore this treatment has a promising potential as a novel and cost-effective castration method with the additional benefit of retaining both testes.

APOPTOSIS IN IPSILATERAL KIDNEY. COMPARISON BETWEEN GROUP RECEIVING VERAPAMIL AND CONTROL GROUP POST-ARTIFICAL TOTAL UNILATERAL URETERAL OBSTRUCTION Syahrir, Syakri; Soetojo, Soetojo; Santoso, Adi; Joewarini, Endang; P, Widodo J
Indonesian Journal of Urology Vol 17 No 1 (2010)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v17i1.340

Objective: Obstruction of the urinary tract has marked effects on renal blood flow, glomerular filtration rate (GFR), and tubular function. Ureteral obstruction results in an injury response that can progress to irreversible renal fibrosis and tubular damage by apoptosis. Materials and Methods: Forty five rabbits aged 13-17 weeks with body weights of 1250-1750 grams were divided into 4 groups. Group 1 underwent a sham operation and group 2 had unilateral ureteral ligation to cause total obstruction. Groups 3 and 4 also underwent unilateral ureteral ligation but with verapamil given on day 0 and day 7 respectively. Apoptosis to the renal tubules were assessed after nephrectomy on day 14 using immunohistochemistry by counting the number cell deaths/high power field (hpf). Results: The groups that received verapamil showed significantly less apoptosis compared to those without verapamil (2,73 vs 12,46 cell deaths/hpf; p<0,05). However, there was no significant difference between groups 3 and 4 (2,73 vs 2,89 cell deaths/hpf; p>0,05), although both groups still showed more cell deaths compared to group 1 (0,38 cell deaths/hpf). Conclusion: Verapamil appears to significantly decrease apoptosis during total unilateral ureteral obstruction. However, it cannot replace the benefit of relieving total obstruction

COMPARISON OF APOPTOSIS IN CONTRALATERAL RENAL TUBULAR CELLS IN ORYCTALAGUS CUNICULUS DUE TO ARTIFICIAL UNILATERAL URETERAL OBSTRUCTION, WITH AND WITHOUT VERAPAMIL Boetoro, Djoyo M; Soetojo, Soetojo; Soebadi, Doddy M; Joewarini, Endang; P, Widodo J
Indonesian Journal of Urology Vol 17 No 2 (2010)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v17i2.344

Objective: To compare the effects of verapamil on the contralateral kidney affected by unilateral ureter obstruction. Material & method: The right ureter of oryctalagus cuniculus rabbits, were obstructed surgically with silk suture material, and kept alive for 14 days. One group was obstructed without other treatment, one group was given verapamil from day 7 until day 14 of the obstruction. One group was obstructed and given verapamil from day 0 through day 14. One group was given a sham operation as a control group. The contralateral kidney of all groups were harvested and processed with ApopTag. The increase of apoptotic tubular cells compared to the control group was then analyzed between each group. Results: The highest increase in apoptotic cells was in the obstructed group without verapamil, but increased significantly compared to control (p<0,001). The groups which received verapamil had lower increase of apoptotic tubular cells, in the group given verapamil for 14 days lower than the group which received verapamil only for 7 days. Both the 7 to 14 and the 0 to 14 groups were significantly lower than the group without verapamil (p=0,035 and p<0,001 respectively). Conclusion: verapamil has a protective effect on the contralateral kidney by inhibiting apoptosis caused by unilateral ureter obstruction. While the definitive treatment for urinary obstruction is to relieve it, verapamil can protect the kidney in the mean time.

Ekspresi p53 Mutant dan EGFR pada Benign, Borderline, dan Malignant Phyllodes Tumor Dian Y. Lestari; Endang Joewarini
Majalah Patologi Indonesia Vol 24 No 2 (2015): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.088 KB)

Latar belakang Tumor phyllodes pada payudara termasuk neoplasma stromal-epitelial yang jarang ditemui, yang secara histologis dapat dibedakan menjadi benign, borderline, dan malignant. Sampai saat ini patogenesis terjadinya tumor phyllodes masih belum jelas. Epidermal growth factor receptor (EGFR) merupakan salah satu reseptor sel di permukaan yang sampai sekarang diyakini memiliki peran dalam proses patogenesis dan progresifitas dari suatu keganasan. Mutasi dari gena P53 (tumor suppressor gene) umum terjadi pada berbagai macam keganasan, salah satunya pada kanker payudara. Tujuan penelitian ini untuk memahami hubungan ekspresi p53 dan EGFR pada benign, borderline dan malignant phyllodes tumor Metode Rancangan penelitian yang digunakan adalah cross sectional. Sampel dibagi dalam; 10 benign, 5 borderline dan 7 malignant phyllodes tumor yang telah didiagnosis di Rumah Sakit Dr. Soetomo Surabaya periode 2009-2012. Pada sampel dilakukan pulasan immunohistokimia dengan antibodi monoklonal p53 dan EGFR serta dinilai menggunakan metode semikuantitatif. Perbedaan ekspresi p53 dan EGFR pada benign, borderline dan malignant phyllodes tumor dianalisis menggunakan Kruskal-Wallis. Hubungan antara ekspresi p53 dan EGFR pada tumor phyllodes dianalisa menggunakan Spearman. Hasil Didapatkan perbedaan ekspresi EGFR yang signifikan pada benign, borderline, dan malignant phyllodes tumor (p=0,022, p

Ekspresi E-cadherin dan Matrix Metalloproteinase-9 pada Invasi Miometrium Endometrioid Adenokarsinoma Endometrium Grace Ariani; Endang Joewarini
Majalah Patologi Indonesia Vol 24 No 3 (2015): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.877 KB)

ABSTRAK Latar belakang Invasi adalah petanda biologi tumor ganas yang berperan dalam proses metastasis. E-cadherin merupakan mediator hubungan antar sel. Matrix metalloproteinase (MMP)-9 merupakan salah satu enzim protease yang berperan dalam degradasi lokal membran basalis serta jaringan ikat. E-cadherin berperan penting dalam adhesi antar sel epitel dan mekanisme arsitekstur jaringan, sedangkan MMP-9 berperan dalam menciptakan jalur untuk migrasi sel tumor. Ekspresi E-cadherin dan MMP-9 berhubungan dengan potensi terjadinya invasi ke dalam miometrium pada kasus endometrioid adenokarsinoma endometrium. Tujuan penelitian ini untuk menganalisis ekspresi E-cadherin dan MMP-9 terhadap invasi miometrium pada endometrioid adenokarsinoma endometrium. Metode Rancangan penelitian yang digunakan adalah cross sectional. Sampel dibagi menjadi 2 yaitu invasi kurang dari setengah ketebalan miometrium dan invasi lebih dari setengah ketebalan miometrium pada penderita endometrioid adenokarsinoma endometrium yang didiagnosis di RSUD Dr. Soetomo mulai Januari 2010-Desember 2011 yang telah memenuhi kriteria inklusi. Sampel dipulas imunohistokimia dengan antibodi E-cadherin dan MMP-9 lalu dinilai secara semikuantitatif. Perbedaan ekspresi E-cadherin dan MMP-9 terhadap invasi miometrium pada endometrioid adenokarsinoma endometrium dianalisis menggunakan uji Mann-Whitney. Hubungan antara ekspresi E-cadherin dan MMP-9 pada endometrioid adenokarsinoma endometrium dianalisis menggunakan uji Spearman. Hasil Analisa statistik ekspresi E-cadherin dan MMP-9 terhadap invasi miometrium pada endometrioid adeno-karsinoma menunjukkan hubungan bermakna (p

Analisis Ekspresi Cyclin D1 dan COX-2 pada Berbagai Grading Histopatologik Karsinoma Endometrioid Ovarium Erwin Sunardi; Endang Joewarini
Majalah Patologi Indonesia Vol 29 No 2 (2020): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

BackgroundOvarian endometrioid carcinoma accounts for 10-15% of ovarian carcinomas, representing the second most common form ofovarian epithelial malignancy. Epithelial cell lines stably transfected to overexpress COX-2 exhibit a higher proliferation rate and aninhibition of apoptosis by prolongation of the G1 phase of the cell cycle through effects on Cyclin D1. The aim of this study is toanalyzed the correlation and differences between Cyclin D1 and COX-2 expressions in various histopathology grading ovarianendometrioid carcinoma.MethodsAn analitycal obsevational design with cross-sectional approach on sample from 23 paraffin blocks in Anatomical PathologyLaboratory Dr. Soetomo General Hospital. Microscopic slide stained with Cyclin D1 and COX-2 antibody. The imunohistochemistryexpression are evaluated and analyzed using Kruskal Wallis and Spearman test.ResultsThere were no differences Cyclin D1 expression in each grade, there were no differences COX-2 expression in each grade, therewas no correlation between Cyclin D1 and COX-2 expression all histopathologic grading, but have correlation expression in grade 3ovarian endometrioid carcinoma.ConclusionThis study showed that Cyclin D1 and COX-2 expression have no significant differences in various histopathological grading ovarianendometrioid carcinoma, and no correlation between Cyclin D1 and COX-2 expression all grade ovarian endometrioid carcinoma

Systemic Effects of Occlusal Disharmony on Corticosterone Serum Levels in Rattus Norvegicus Suhartini; Endang Joewarini; Ida Bagus Narmada
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.15007

Background: Mastication process occurs over time and it can lead to occlusal reduction of the teeth. Thiscauses reducing occlusal contact of teeth and it will lead to disharmony occlusion disorders. The occlusiondisharmony is recognized by the body as a stressor that systemically affects the homeostasis in the body,especially the production of hormones.Objective: The aim of this study was to determine the systemic effect of occlusal disharmony on corticosteroneserum levels in Rattus norvegicus.Method: The research method of this study used experimental design and conducted among 7 male RattusNorvegicus in each groups. In intervention group, an occlusal reduction was ± 1 mm in all molars andfor control group did not given any intervention. Moreover, at 1st day, 7th day, 14th day and 21st day wastaken 2 cc of blood via infraorbital vein. The measurement of Corticosterone levels used Enzyme LinkedImmunosorbent Assay (ELISA).Result: Anova test results showed that corticosterone levels was significant difference between each groups(p <0.05). Tukey HSD test showed there was a significant difference between intervention group and controlgroup on 1st day, in intervention group on 7th day (p <0.05).Conclusion. The results of this study showed that there were differences of corticosterone serum levelsamong mice who experienced occlusion disharmony so it concluded that the dental occlusion disharmonyhad a systemic impact to corticosterone serum levels in Rattus norvegicus

NFkB and MMP-13 expression in condylar cartilage of temporomandibular joint with occlusal disharmony in vivo Suhartini; Ida Bagus Narmada; Zahreni Hamzah; Endang Joewarini
Dental Journal Vol. 57 No. 1 (2024): March
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v57.i1.p22-27

Background: Temporomandibular disorder (TMD) is a collection of symptoms that causes pain and disturbs a person's life quality. One of the trigger factors is mechanical overloading. Mechanical overloading in occlusal disharmony conditions will lead to an inflammatory reaction in the temporomandibular joint (TMJ). This condition will induce nuclear factor Kappa Beta (NFkB) activation to transcribe proinflammatory cytokines and matrix metalloproteinase-13 (MMP13) and will also degrade condylar cartilage as a major factor in strengthening the TMJ. Purpose: The aim of this study was to analyze the expression of NFkB and MMP13 in the condylar cartilage of TMJ with occlusal disharmony. Methods: This research was an experimental study with post-test-only control group design. Twenty Rats (Rattus norvegicus) were divided into four groups: One control group without any intervention and three experimental groups. Occlusal-reducing intervention was due on the right molar of the experimental groups, which were divided into the 7th, 14th, and 21st days. Immunohistochemical staining was performed to determine the expression of NFkB and MMP13 in the condylar cartilage. The data were analyzed by using the Welch test and independent t-test. Results: There were significant differences in NFkB and MMP13 expression between the control and experiment experimental groups (p<0.05). NFkB expression increased on the 7th, 14th, and 21st days of observation. The MMP13 expression showed a significant difference between the control and experimental groups (p<0.05). Conclusion: Occlusal disharmony increases NFkB and MMP13 expression and could affect TMJ integrity and induce TMD. These findings are important for describing the mechanism of TMJ damage and developing potential alternative therapies to prevent further TMD.

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