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All Journal Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS Cassowary
Ni Made Gari
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana, Jl. Raya Kampus Unud, Bukit Jimbaran, Badung 803611, Bali, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science

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MULTIVARIATE ANALYSIS OF BALI SALAK CULTIVARS (SALACCA ZALACCA VAR. AMBOINENSIS (BECC.) MOGEA) BASED ON LEAF MICROMORPHOLOGICAL CHARACTERS NI MADE GARI
Jurnal Biologi Udayana Vol 15 No 1 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Leaf micromorphology of 13 Bali salak cultivars was studied by using multivariate analysis (Principal component analysis). The results showed that the cultivars clustered into a main group consisted of nine cultivars (Boni, Bingin, Selem, Embad, Nangka, Penyalin, Maong, Nyuh, and Putih). However, two cultivars (Muani and Gondok) were distinctly separated from this main group and two the others Nanas and Gula were intermediate. The principal component analysis (PCA) revealed that the main group has highly correlated to the characters abaxial cell length and number of cross vein density. Muani cultivar generally had longer adaxial cells, wider guard cells and lower stomatal index than the other cultivars. These three characters strongly influenced the separation of Muani from the others. Similarly, Gondok cultivar generally had wider abaxial cells than the other cultivars that discriminated Gondok clearly from the others. Whereas, the intermediate cultivars (Gula and Nanas) were placed between the main group that consisted of nine cultivars and the two separated cultivars (Muani and Gondok). These cultivars (Gula and Nanas) had intermediate values, which influenced their separations.
THE LEAF ANATOMY OF BALI SALAK CULTIVARS (Salacca zalacca var. Amboinensis (Becc.) Mogea) Ni Made Gari
Bumi Lestari Journal of Environment Vol 11 No 2 (2011)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Studi anatomi dilakukan untuk mengetahui karakteristik epidermis daun pada 13 kultivar salak Bali. Hasil penelitian ini menunjukan bentuk sel pada bagian permukaan atas (adaksial) dan permukaan bawah (abaksial) daun salak Bali adalah persegi empat sampai jajaran genjang. Sedangkan dinding antiklinal sel epidermisnya berlekuk-lekuk dengan penebalan pada dinding tersebut. Semua kultivar yang diamati memperlihatkan tipe daun yang hipostomatik dimana stomata hanya dijumpai pada bagian abaksial daun. Walaupun demikian stomata terkadang dapat dijumpai pada bagian adaksial daun meski jumlahnya sangat sedikit. Tipe stomata pada daun salak bali adalah parasitik dimana tiap sel penutup didampingi oleh satu atau lebih sel tetangga yang letaknya sejajar dengan stomata, dengan penebalan pada dinding sel penutupnya. Indeks stomata bervariasi antara 11.9% sampai 18.3% pada semua kultivar yang diamati.
Inventarisasi Dendrobium linearifolium Teijsm. & Binn. Dan Pohon Inangnya Di Kawasan Desa Lemukih, Buleleng - Bali Ni Made Gari; Junita Hardini; I Ketut Ginantra
Bumi Lestari Journal of Environment Vol 22 No 2 (2022)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2022.v22.i02.p05

Abstract

Dendrobium linearifolium Teijsm. & Binn. or "shrimp orchid" is one of the epiphytic orchids that is increasingly rare, therefore ex situ conservation is necessary. The success of ex situ orchid conservation requires knowing the types of its host trees. The study aimed to carry out an inventory of D. linearifolium and its host trees in the Lemukih Village Area, Buleleng – Bali. An explorative method was applied in the research. There were 63 individuals of D. linearifolium found in Lemukih Village. The highest number was present at an altitude of 500-700 meters above sea level (masl) (35), followed by those at 901-1200 m apl (22) and at 701-900 m apl (6) respectively. Meanwhile, 17 host trees were obtained which consisted of 10 species and eight families. Most D. linearifolium were found on Syzygium aqueum (Burm.f.) Alston (11), followed by those on Tamarindus indica L. (10), Helicia robusta (Roxb) R.Br. ex Blume (10) and Coffea canephora L. (7).
ANALISIS KERAGAMAN GENETIK VARIETAS JAGUNG (Zea mays L.) HIBRIDA MENGGUNAKAN MARKA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) Viryanando Evan Rahardja; Made Pharmawati; Ni Made Gari
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.524 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i02.p09

Abstract

Analisis keragaman genetik sangat penting dalam perakitan tanaman jagung (Zea mays L) hibrida. Tujuan dari penelitian ini adalah untuk menganalisis keragaman genetik dari enam varietas jagung menggunakan teknik PCR-RAPD (Polymerase Chain Reaction- Random Amplified Polymorphic DNA). Varietas jagung hibrida yang digunakan dalam penelitian ini adalah varietas jagung Srikandi, Arumba, Mira, Magenta, dan Simba. Varietas jagung lokal yang berasal dari Pulau Rote, Provinsi Nusa Tenggara Timur digunakan sebagai pembanding. Benih ditanam untuk pengamatan karakteristik morfologi dan sampel ekstraksi DNA. DNA diekstraksi dengan metode CTAB dan PCR-RAPD dilakukan menggunakan 3 primer. Ukuran produk PCR ditentukan menggunakan kertas semilog. Keragaman genetik antar varietas dianalisis menggunakan program MVSP (Multi-Variate Statistical Package) dengan metode UPGMA (Unweighted Pair Group Method with Arithmetic Mean). Hasil PCR-RAPD memberikan jumlah pita DNA sebanyak 28 dengan ukuran 310 bp hingga 1550 bp bp dan menghasilkan 23 pita polimorfik. Berdasarkan dendrogram yang dihasilkan dari PCR-RAPD, terdapat satu klaster yang terdiri atas varietas Rote, Srikandi, Arumba, Mira, dan Magenta, serta satu klaster lain yang terdiri atas varietas Simba. Varietas lokal Rote dan varietas hibrida Srikandi memiliki jarak genetik terdekat, sementara varietas Simba memiliki jarak genetik terjauh.
Pertumbuhan dan hasil tanaman sawi (Brassica rapa L.) sebagai respon terhadap jarak tanam pada dua periode tanam Saraswati P; Syrylus Keupung; Inna Rumainum; Sutiharni Sutiharni; Fenny Asyerem; Linda E. Lindongi; Marnangon Tambunan; Ni Made Gari
Cassowary Vol 6 No 1 (2023): Januari
Publisher : Program Pascasarjana Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/casssowary.cs.v6.i1.212

Abstract

Mustard consumption increases due to population growth, therefore it is necessary to increase its yield. One of the cultivation techniques to increase the growth and yield of green mustard can be done by the right plant spacing. This study aims to examine several plant spacings in two planting periods on the growth and yield of green mustard. The research was carried out from April to June 2020, at the Experimental Field of Agriculture Faculty Papua University, Manokwari, West Papua. This study was laid in a Randomized Block Design (RAK), with 3 spacing treatments, namely 25 cm x 15 cm, 25 cm x 20 cm and 25 cm x 30 cm. Each treatment was repeated 5 times. The data was analyzed using analysis of variance at the 95% confidence level, and if it had a significant effect, it was continued with the Least Significant Difference Test (BNT) at the 95% level. To see the comparison of mustard yield between the first and second studies, tabulated analysis was used. The results of the first and second studies showed that the three treatments tested had a significant and very significant effect on the character of plant weight per plant, per plot, and yield per hectare. The 25 cm x 15 cm plant spacing resulted in the highest yield per plot or per hectare. Plant spacing of 25 cm x 15 cm was 12 tons/ha produced the highet yield. On the other hand, a spacing of 25 cm x 30 cm produced the highest yield per plant. The growth and yield components decreased in the second study, except at a spacing of 25 cm x 15 cm which capable of producing 10 tons/ha.
EFFECTIVENESS OF CINNAMON (Cinnamomum burmanni Blume) LEAF EXTRACT IN INHIBITING THE GROWTH OF THE MUSHROOMS Colletotrichum magnum Rossman & Allen CAUSES OF ANTHRACHNOSE DISEASE IN PAPAYA (Carica papaya Linnaeus) Wafiatul Fitriyah; Sang Ketut Sudirga; Ni Made Gari
SIMBIOSIS Vol 11 No 1 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i01.p09

Abstract

Anthracnose is a disease which is often found attacking plants. This disease can cause dieback or leaf tip damage of mature plants then followed by infection in the fruits, Consequently, this can result in the decrease of productivity. One of the causes of anthracnose is the pathogenic fungus Colletotrichum magnum. This fungus can infect several cultivated plants, one of which is papaya plants. This study aimed to determine the effectiveness of cinnamon leaf extract (Cinnamomum burmanni. Blume) in suppressing the growth of the fungus C. magnum and to analyze the class of compounds contained in cinnamon leaf extract. The method used in this study was a Completely Randomized Design (CRD) with 1 negative control, 1 positive control, and 5 treatments with concentrations of 1%, 2%, 3%, 4%, 5%, each treatment was repeated 4 times and then tested phytochemical screening. The results of the invitro study showed that cinnamon leaf extract was able to inhibit the growth of C. magnum as seen from the MIC test results, namely a concentration of 0.6% with an inhibition zone diameter of 8.37 mm. The results showed that as the concentration of cinnamon leaf extract increased, the diameter of the inhibition zone obtained was getting bigger. what you get is bigger. The concentration of 5% that has the largest inhibition zone among the concentration treatments, which is 19.77 mm. The results of phytochemical screening on cinnamon leaf extract showed that the cinnamon leaf extract contains positive groups of alkaloids, steroids, phenolic compounds, saponins, flavonoids, and tannins. Keyword: anthracnose, Cinnamon leaves, Inhibition, Colletotrichum magnum.
Karakteristik Polen pada Spesies Mangrove di Kawasan Hutan Mangrove Batu Lumbang, Denpasar, Bali Pramesti, Ni Kadek Rika; Wijaya, I Made Saka; Gari, Ni Made
Bumi Lestari Journal of Environment Vol 24 No 1 (2024)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2024.v24.i01.p05

Abstract

The diversity of mangrove species in an area can be observed through morphological structure, anatomy, as well as palynology. Palynology is a study of pollen that frequently used as a reference to support data in identifying mangrove species. Mangrove areas in Bali have patchy distribution, one of which can be found in the Batu Lumbang area. Batu Lumbang is a mangrove area that is part of the Ngurah Rai Forest Park. Observation of the micromorphological characteristics of pollen in the Batu Lumbang mangrove forest area was carried out through the acetolysis method with modification. Eleven mangrove species were sampled, comprised of Aegiceras corniculatum, Avicennia marina, Bruguiera gymnorhiza, Ceriops tagal, Exoecaria agallocha, Lumnitzera racemosa, Rhizophora apiculata, Rhizophora mucronata, Sonneratia alba, Sonneratia caseolaris, and Xylocarpus granatum. The results of observations on pollen from eleven species of mangroves show similarities of pollennits, monads. In addition, other pollen characters such as aperture type, pollen shape, and pollen size categories found vary among the samples observed. These characters can also group several species that have the same family or genus. In turns, these can be used as diagnostic character to distinguish one species with another.
Co-Authors Fenny Asyerem I Ketut Ginantra Inna Rumainum Junita Hardini Linda E. Lindongi MADE PHARMAWATI Marnangon Tambunan Pramesti, Ni Kadek Rika SANG KETUT SUDIRGA Saraswati P Sutiharni Syrylus Keupung Viryanando Evan Rahardja Wafiatul Fitriyah Wijaya, I Made Saka