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All Journal JOURNAL OF COASTAL DEVELOPMENT Jurnal Akademika Biologi OSEANA
Yeti Darmayati
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Biochemistry, Genetics & Molecular Biology Education Environmental Science Other

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PENDEKATAN CULTURE INDEPENDENT UNTUK ANALISIS KOMUNITAS BAKTERI Nur Fitriah Afianti; Yeti Darmayati
OSEANA Vol 42 No 1 (2017)
Publisher : Pusat Penelitian Oseanografi LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oseana.2017.Vol.42No.1.34

Abstract

CULTURE INDEPENDENT APPROACH FOR BACTERIAL COMMUNITY ANALYSIS. Analysis of bacterial community can be through two approaches, through cultivation (culture dependent) and without cultivation (culture independent). Culture dependent approach is conventional method which only covered few bacteria because not all bacteria could be cultured. Culture independent approach with molecular techniques based on DNA communities can provide more information about the structure and diversity of bacteria in nature, both culturable bacteria and unculturable bacteria. 16S rRNA gene is commonly target gene used in bacterial communities analysis. Other specific target genes also being developed for specific groups of bacteria. Several methods are developed for the analysis of molecular markers 16S rRNA or other specific genes, including Denaturing Gradient Gel Electrophoresis (DGGE), Terminal Restriction Fragment Length Polymorphism (TRFLP), and Single Strand Conformation Polymorphism (SSCP).
PENERAPAN DAN TINGKAT EFEKTIVITAS TEKNIK BIOREMEDIASI UNTUK PERAIRAN PANTAI TERCEMAR MINYAK Yeti Darmayati; Nur Fitriah Afianti
OSEANA Vol 42 No 4 (2017)
Publisher : Pusat Penelitian Oseanografi LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oseana.2017.Vol.42No.4.73

Abstract

APPLICATION AND EFFECTIVITY OF BIOREMEDIATION TECHNIQUE FOR OIL CONTAMINATED COASTAL WATERS. Oil pollution is often occurs in Indonesian marine waters and it has a significant impact on ecological and economical aspects. Physical and chemical approaches are common to be used to combat this pollution. Bioremediation is a promising method using biological approach that not been used yet for oil spill clean up in Indonesian coastal environment. The success stories were reported mostly from sub-tropic region. This technique is potential to be used in Indonesian due to tropical climates and enormous microbial resources in Indonesian marine waters. However, intensive research and field test are still needed to develop an appropriate method for application in Indonesia. One of bioremediation technique limitations is the suitability for each polluted sites. This paper will discuss about the application and the affectivity of bioremediation technique in other countries and research development of this technique for coastal environment in Indonesia.
Co-Authors Agung Suprihadi Endang Kusdiyantini Linda Safitri Nur Fitriah Afianti