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Nur Fitriah Afianti
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Biochemistry, Genetics & Molecular Biology Environmental Science Other

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POTENSI BAKTERI LAUT UNTUK BIOREMEDIASI Nur Fitriah Afianti
OSEANA Vol 43 No 4 (2018)
Publisher : Pusat Penelitian Oseanografi LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oseana.2018.Vol.43No.4.6

Abstract

POTENCY OF MARINE BACTERIA FOR BIOREMEDIATION. Marine bacteria have a high abundance on variation as well as the qenes, so it can be used in variousaspects, including the prevention of marine pollution through bioremediation. High genetic variations provide a source of marine bacteria which has decompose-various substances capability. Various studies have been conducted to determine the potential of marine bacteria in degrading pollutants, including degradation of hydrocarbons, PAH, plastic, PCB and removal of heavy metal. Recombination through the insertion of the marine bacteria’s gene coding enzyme has been also performed on indigenous actinomycetes on soil.
PENDEKATAN CULTURE INDEPENDENT UNTUK ANALISIS KOMUNITAS BAKTERI Nur Fitriah Afianti; Yeti Darmayati
OSEANA Vol 42 No 1 (2017)
Publisher : Pusat Penelitian Oseanografi LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oseana.2017.Vol.42No.1.34

Abstract

CULTURE INDEPENDENT APPROACH FOR BACTERIAL COMMUNITY ANALYSIS. Analysis of bacterial community can be through two approaches, through cultivation (culture dependent) and without cultivation (culture independent). Culture dependent approach is conventional method which only covered few bacteria because not all bacteria could be cultured. Culture independent approach with molecular techniques based on DNA communities can provide more information about the structure and diversity of bacteria in nature, both culturable bacteria and unculturable bacteria. 16S rRNA gene is commonly target gene used in bacterial communities analysis. Other specific target genes also being developed for specific groups of bacteria. Several methods are developed for the analysis of molecular markers 16S rRNA or other specific genes, including Denaturing Gradient Gel Electrophoresis (DGGE), Terminal Restriction Fragment Length Polymorphism (TRFLP), and Single Strand Conformation Polymorphism (SSCP).
PENERAPAN DAN TINGKAT EFEKTIVITAS TEKNIK BIOREMEDIASI UNTUK PERAIRAN PANTAI TERCEMAR MINYAK Yeti Darmayati; Nur Fitriah Afianti
OSEANA Vol 42 No 4 (2017)
Publisher : Pusat Penelitian Oseanografi LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oseana.2017.Vol.42No.4.73

Abstract

APPLICATION AND EFFECTIVITY OF BIOREMEDIATION TECHNIQUE FOR OIL CONTAMINATED COASTAL WATERS. Oil pollution is often occurs in Indonesian marine waters and it has a significant impact on ecological and economical aspects. Physical and chemical approaches are common to be used to combat this pollution. Bioremediation is a promising method using biological approach that not been used yet for oil spill clean up in Indonesian coastal environment. The success stories were reported mostly from sub-tropic region. This technique is potential to be used in Indonesian due to tropical climates and enormous microbial resources in Indonesian marine waters. However, intensive research and field test are still needed to develop an appropriate method for application in Indonesia. One of bioremediation technique limitations is the suitability for each polluted sites. This paper will discuss about the application and the affectivity of bioremediation technique in other countries and research development of this technique for coastal environment in Indonesia.
MIKROBA PENCEMAR DI PERAIRAN TELUK JAKARTA Nur Fitriah Afianti; Lies Indah Sutiknowati
OSEANA Vol 42 No 3 (2017)
Publisher : Pusat Penelitian Oseanografi LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oseana.2017.Vol.42No.3.83

Abstract

MICROBE CONTAMINANT IN JAKARTA BAY WATERS. High anthropogenic activity in Jakarta Bay is believed to contribute a fairly high contamination as evidenced by microbial contaminants such as Coliform and Escherichia coli. The high density of Coliform and E. coli as indicators of domestic contamination in Jakarta Bay as a result of an increase in the resident numbers who bring the flow of water to the springhead of the Jakarta Bay. It can be said that the polluted waters of Jakarta Bay and marine biota should be cultivated in this area as green shell should not consumed, because it can infect and cause gastrointestinal diseases. For that purpose, a research of microbial pollutants was conducted in Jakarta Bay in July 2015, and the results showed a very high density, especially in the West.
Co-Authors Lies Indah Sutiknowati Yeti Darmayati