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All Journal Reaktor Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya
Astri Nur Istyami
Institut Teknologi Bandung
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Energy Materials Science & Nanotechnology

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Performance of Various Organic Solvents as Reaction Media in Plant Oil Lipolysis with Plant Lipase Astri Nur Istyami; Tatang Hernas Soerawidjaja; Tirto Prakoso; Tri Ari Penia Kresnowati
Reaktor Volume 18 No. 2 June 2018
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (651.652 KB) | DOI: 10.14710/reaktor.18.2.71-75

Abstract

Fatty acids are intermediate substances in synthesis of oleochemical products. Enzymatic technology of fatty acids production (also known as lipolysis) is now developing as potential substitution for the conventional production of fatty acid, i.e. thermal hydrolysis of triglyceride. It offers more economical process condition, low energy consumption, and minimal product degradation compared to the conventional process. This research aims to evaluate performance of various organic solvents as reaction media in lipolysis with plant latex lipase. Organic solvents observed were chloroform, n-hexane, diethyl ether, benzene, acetone, ethanol, methanol, n-heptane, and isooctane. Analysis of each organic solvent effect on lipolysis was described based on solvents properties. Conversion of lipolysis with organic solvents is 0,10-1,25 times fold compared to conversion of non-solvent lipolysis. We suggest that dielectric constant and viscosity are the two main organic solvent properties affecting lipase performance in lipolysis. Overall, n-hexane, n-heptane, and isooctane are recommended to be used as reaction media in lipolysis with plant lipase because their effects to degree of lipolysis are positive.  Keywords: lipolysis; lipase; organic solvent; frangipani
PEMISAHAN GLISERIDA-GLISERIDA SISA DARI ASAM LEMAK HASIL LIPOLISIS Dinda Rizka Fadhillah; Astri Nur Istyami; Sry Wahyuni
Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya Vol. 7 No. 2 (2025): Literasi Artikel Penelitian Kimia
Publisher : Program Studi Kimia, Fakultas MIPA, Universitas PGRI Banyuwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/jc.v7i2.6132

Abstract

Produksi asam-asam lemak hasil lipolisis minyak sawit dapat diperoleh diatas 60%. Proses pemisahan gliserida-gliserida sisa dari asam-asam lemak hasil lipolisis harus dikembangkan untuk mendukung paket teknologi penyediaan asam-asam lemak dalam industri oleokimia dan juga dalam pembuatan ester metil asam-asam lemak. Ekstraksi dengan pelarut merupakan proses pemisahan yang tepat untuk memisahkan gliserida sisa dari asam-asam lemak karena gliserida memiliki titik didih yang sangat tinggi. Pelarut yang digunakan adalah diklorometan, dietil eter campuran diklorometan-dietil eter, dan asetonitril. Ekstraksi dioperasikan pada suhu 40-45oC selama 15-30 menit dan diaduk dengan putaran sebesar 150-200 rpm. Penelitian yang telah dilakukan menunjukkan bahwa ekstraksi hasil lipolisis pelarut diklorometan-dietil eter tak menunjukkan hasil-hasil yang memuaskan. Hasil yang paling bagus menghasilkan angka asam ekstrak dan rafinat 131,8 dan 172,7 mg KOH/g sampel. Dari hasil penelitian yang diperoleh, penggunaan asetonitril dipandang paling layak untuk ditelusuri lebih lanjut dengan angka asam ekstrak dan rafinat sebesar 157,5 dan 62,6 mg KOH/g sampel.
Co-Authors Dinda Rizka Fadhillah M.T.A.P. Kresnowati Sry Wahyuni Tatang Hernas Soerawidjaja Tirto Prakoso