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16S rRNA Gene Analysis of Chlorate Reducing Thermophilic Bacteria from Local Hot Spring Aminin, Agustina L. N.; Katulistiwasari, Puri; Mulyani, Nies Suci
JURNAL SAINS DAN MATEMATIKA Volume 19 Issue 4 Year 2011
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3028.349 KB)

Abstract

Chlorates waste remediation by biological processes has been the object of current research. Strain CR, the chlorate reducing bacteria was isolated from Gedongsongo hot spring using minimal medium broth containing chlorates and acetate at 55oC. The determination of chlorate reduction from medium was carried out using turbidimetric method. CR isolate showed reducing ability 18% after four days of incubation. The phenotypic character of CR isolate including rod-shaped cells, gram-positive bacteria and facultative anaerobes. On the basis of 16S rRNA analysis, CR isolate was closely related to Bacillus pallidus, Aeribacillus E3, Geobacillus pallidus and Geobacillus sp.D64. The similarity of nucleotide sequences of 16S rRNA gene was 97%, suggested as a novel species.   Keyword: thermophilic bacteria, chlorate reducing, 16S rRNA gene
Penerapan Pembelajaran Mendalam untuk Penguatan Kompetensi Design Thinking Siswa SMK Muhammadiyah 2 Tangerang Selatan S, Sahrudin; Putri, Dianasyah; Pratama, Hady Putra; M, Masitoh; Harahap, Nursakina; Katulistiwasari, Puri; Jaya, Herdi Wisman
Nanggroe: Jurnal Pengabdian Cendikia Vol 4, No 9 (2025): December 2025
Publisher : Yayasan Daarul Huda Kruengmane

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.18067911

Abstract

The low level of critical and creative thinking skills among vocational high school students has contributed to the high rate of open unemployment. This community service program aimed to strengthen students’ design thinking competence at SMK Muhammadiyah 2 Tangerang Selatan through a deep learning approach. The activity employed a participatory, project-based training method consisting of the stages of empathize, define, ideate, prototype, and test. The results showed an improvement in students’ understanding of design thinking, active participation, and ability to generate solutions to real-world problems within the school environment. This program also contributed to strengthening the Pancasila Student Profile, particularly in critical reasoning, creativity, collaboration, and independence.
Penerapan Pembelajaran Mendalam untuk Penguatan Kompetensi Design Thinking Siswa SMK Muhammadiyah 2 Tangerang Selatan S, Sahrudin; Putri, Dianasyah; Pratama, Hady Putra; M, Masitoh; Harahap, Nursakina; Katulistiwasari, Puri; Jaya, Herdi Wisman
Nanggroe: Jurnal Pengabdian Cendikia Vol 4, No 9 (2025): December 2025
Publisher : Yayasan Daarul Huda Kruengmane

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.18067911

Abstract

The low level of critical and creative thinking skills among vocational high school students has contributed to the high rate of open unemployment. This community service program aimed to strengthen students’ design thinking competence at SMK Muhammadiyah 2 Tangerang Selatan through a deep learning approach. The activity employed a participatory, project-based training method consisting of the stages of empathize, define, ideate, prototype, and test. The results showed an improvement in students’ understanding of design thinking, active participation, and ability to generate solutions to real-world problems within the school environment. This program also contributed to strengthening the Pancasila Student Profile, particularly in critical reasoning, creativity, collaboration, and independence.