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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Scripta Biologica
Boy Rahardjo Sidharta
Universitas Atma Jaya Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Karakteristik dan Efektivitas Salep Madu Klanceng dari Lebah Trigona sp. Sebagai Antibakteri dan Penyembuh Luka Sayat Martin Aristo Cahyadi; Boy Rahardjo Sidharta; Nelsiani To'bungan
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 4, No 3 (2019): October 2019
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v4i3.2520

Abstract

Penggunaan antibiotik secara terus menerus dapat menimbulkan resistensi pada bakteri. Salah satu bahan alami yang dapat menjadi alternatif pengobatan adalah madu klanceng. Penelitian ini bertujuan untuk mengetahui sediaan salep madu klanceng yang optimal dan efeknya terhadap penyembuhan luka dan infeksi yang disebabkan oleh bakteri. Tahapan penelitian ini terdiri dari uji fitokimia madu, pembuatan salep, uji sediaan salep, uji kemurnian bakteri, uji zona hambat, dan uji bioassay pada kelinci. Madu klanceng yang diuji mengandung senyawa alkaloid. Variasi salep madu dibuat dengan perbandingan basis salep dan madu adalah 1:1, 1:2, 1:5, dan 5:1. Hasil salep berbentuk semi padat, berwarna  kuning, pH sekitar 4,8, belum homogen, memiliki daya lekat sekitar 265,4 detik, dan daya sebar sebesar 1,76 cm. Salep madu mampu menghambat bakteri S. aureus dan P. aeruginosa dengan diameter sebesar 1,2 mm. Salep madu ini juga memberikan efek menyembuhkan luka hingga 0,3 cm.
AKTIVITAS ANTIBAKTERI EKSTRAK KELOPAK BUAH DENGEN (Dillenia serrata Thunb.) TERHADAP Escherichia coli DAN Staphylococcus aureus Boy Rahardjo Sidharta
Scripta Biologica Vol 6, No 1 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

AbstRactDillenia serrata (Dengen) is an endemic plant from Sulawesi and it has never been known for its benefit, especially as antibacterial herbs. Unripe Dengen’s fruit is enveloped by sepals, thus it is indicated to have antibacterial activity as a protection of fruit from destructive bacteria. This research aimed to determine the optimum inhibitory of Dengen fruit sepals extracts to Escherichia coli and Staphylococcus aureus. Fruit sepals were extracted with two solvents, which are polar and non-polar, namely methanol and n-hexane. The extraction was done by maceration. Compounds from methanol and n-hexane extracts were qualitatively measured using phytochemical test, such as alkaloid, flavonoid, tannin, triterpenoid/steroid, and saponin. Methanol and n-hexane extracts were divided into three concentrations as treatments for antibacterial test namely 30, 60, and 90 % using disk diffusion method with five replications. The result of phytochemical test showed Dengen fruit sepals contained alkaloids, flavonoids, tannins, triterpenoids/steroids and saponins. The extracts showed optimum inhibitory effect to E. coli which was obtained at 90 % of methanol extract with 0.495 cm inhibitory zone diameter. While, the optimum inhibitory effect to S. aureus was obtained at 90 % of n-hexane extract with 0.54 cm of inhibitory zone diameter. Key words: Dengen fruit sepals, antibacterial, maceration, phytochemical, disk diffusion method
Co-Authors Martin Aristo Cahyadi To'bungan, Nelsiani