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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kimia Terapan Indonesia
Lenny Sutedja
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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Analisis kandungan artemisinin pada kultur tunas Artemisia annua L. dengan lima karakter morfologi yang berbeda Tri Muji Ermayanti; Erwin Al Hafiizh; Aryanti Aryanti; Lenny Sutedja
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2875

Abstract

Artemisia annua L. is a medicinal plant that has an antimalarial drug againts Plasmodium falciparum strains which are resistant to traditional drugs such as quinine and chloroquine. This plant contains artemisinin as major compounds found on its leaves. Aim of the research was to analyze the artemisinin content of shoot culture of A. annua which has five different in leaf morphologies (Morphology type A, B, C, D and E). Previous investigation showed that shoot regenerated from callus has five different morphologies. This somaclonal variation was permanent after continuously subcultured onto MS medium with no addition of plant growth regulators. Artemisinin was detected using HPLC and it was comfirmed by LC-MS. The results showed that shoot culture type B contained the highest artemisinin content (0.171%) amongst other type of morphology. Shoot type D showed the lowest level of artemisinin (0.006%) which was not significantly different with type E (0.008%). Addition of BAP at concentration of 1 and 2 mg/l increased artemisinin level of the shoot culturs.
ISOLATION AND IDENTIFICATION OF SIMIARENOL FROM THE LEAVES OF FICUS AURANTIACEA GRIFF (MORACEAE) L. B.S. Kardono; Lenny Sutedja; H. H.S. Fong; S-X Qiu
Jurnal Kimia Terapan Indonesia Vol 10, No 1-2 (2000)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2734.292 KB) | DOI: 10.14203/jkti.v10i1-2.177

Abstract

Ficus aurantiace a Griff (Mor aceae) collected from BukitKaba forest park, Bengkulu, was studied for the isolation andidentification of its bioactive constituents. This plant is a climbingtree, having large colored fruits, orange to red marbled withwhite spots. The methanol-soluble extract of the leaves was eluted10 silica-gel column chromatography several times using mixturesof chloroform-methanol as the elution solvent, leading to theisolation of a known compound, simiarenol. The structure ofsimiarenol was judged by its data comparison to published values,and based all the evaluation results of its physical andspectroscopic data. such as UV, IR, MS, one- and two-dimensional NMR, HMQC and HMBC.
EKSTRAKSI DAN FRAKSINASI KOMPONEN BIOAKTIF ANTIMIKROBA DALAM BIJI INTISARI DAN DAUN LADA Lenny Sutedja; Herlina Agustina
Jurnal Kimia Terapan Indonesia Vol 4, No 2 (1994)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4328.333 KB) | DOI: 10.14203/jkti.v4i2.286

Abstract

In the framework of antimicrobial activity investigation, seeds and leaves of black pepper were fractionated with n-hexane, methanol, chloroform and water consecutively, to isolate the active fractions. The antimicrobial activity was determined by agar plate diffusion method against Staphylococcus aureus ATCC 6536, Escherichia coli ATCC 25922 and Candida albicans ATCC 10231. Antimicrobial activity against the microbes was not found in the pepper leaf extracts as well as in the n-hexane extract of the seeds. Methanol, chloroform and water extracts of pepper seeds showed antimicrobial activity against S.aureus and Calblcans, however they did not show any activity against E.coli. The chloroform extract, which was the most active, was further eluted and fractionated by column chromatography on silica gel, eluted gradually with n-hexanetethylacetate. Antimicrobial activity determination of the fractions obtained from the column chromatography, indicated that fraction L(IV) showed the highest activity. Gas chromatography-mass spectrometry analysis of L(IV) illustrated the possibility of the presence of several compounds, such as piperonal; palmitic acid, stearic acid, oleic acid, B-sitosterol and 2(3H)-furanone, 3,4-bis (l,3-benzodioxol-5-ylmetyl) dihydro.
PENAPISAN AKTIVITAS ANTIPROTOZOA DALAM BIJI SAGA (Adenanthera pavonina LINN) Lenny Sutedja
Jurnal Kimia Terapan Indonesia Vol 5, No 1 (1995)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3993.713 KB) | DOI: 10.14203/jkti.v5i1.246

Abstract

The protozoa Tetrahymena pyriformis GL is an eucaryote. Its metabolism is similar to that of mammalia, so that it is widely used as a biological tool in protein quality as well as toxicity assays. In the framework of searching toxic or antinutritive properties in saga seed (Adenanthera pavonina LINN), extracts of saga bean were tested for their antiprotozoa activity. The saga bean extracts were obtained after successive extraction with nhexane and ethanol. Observation of total and motile cell population indicated that 0,1% saga oil in the medium did not show significant effect on the growth of T.pyriformis GL during 96 hours incubation at 30°C. While ethanol extract of saga showed significant inhibition on the growth of T.pyriformis GL. Smaller cell population was already observed at 7 hours incubation at 30°C. At 24 hours incubation, 0,1% and 1% ethanol extract of saga in the medium showed 55,1% and 87,6% inhibition respectively. Ethanol extract showed the largest anttprotozoal activity compared to the other extract. Qualitative analysis indicated the presence of saponin and alkaloid in the ethanol extract of saga bean. Chromatographic analysis with high performance liquid chromatography showed the presence of at least eight components in the ethanol extract of saga.
Co-Authors aryanti aryanti Erwin Al Hafiizh H. H.S. Fong Herlina Agustin L. B.S. Kardono S-X Qiu Tri Muji Ermayanti