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All Journal Pharmaciana: Jurnal Kefarmasian
Nurkhasanah Mahfudh
Fakultas Farmasi, Universitas Ahmad Dahlan
Medicine & Pharmacology

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Chloroform fraction of ethanolic extract of Elephantopus scaber Linn. increase the p53 expression on human breast cancer (T47D) cell line Nurkhasanah Mahfudh; Nanik Sulistyani; Lukman Mahdi
Pharmaciana Vol 7, No 2 (2017): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.845 KB) | DOI: 10.12928/pharmaciana.v7i2.6558

Abstract

Elephantopus scaber Linn had been used as traditional medicine. It contain some lacton sesquiterpene with antiproliferative activity. The study aim was to explore the effect of chloroform fraction of ethanolic extract of E.scaber on the expression of p53 on T47D cell line. The ethanol extract was found by maceration method. The extract was then fractionated using petroleum ether and chloroform respectively to get the chloroform fraction. The expression of p53 was observed by immunocytochemistry method with antip53 antibody. The p53 cell line was cultured and treated with fraction with concentration of 7.06 and 3.53 µg/ml. The expression of p53 was showed by brown colour in the nucleus of the cell. The result showed that chloroform fraction treatment with concentration of 7.06 µg/ml induced apoptotic cell death in the end stage (necrosis). While the treatment with lower concentration 3.53 µg/ml could increase the p53 expression.
The effect of Zingiber cassumunar Roxb rhizome extract on in vitro phagocytic activity and lymphocyte proliferation Nurkhasanah Mahfudh; Nanik Sulistyani; Dinda Anindya Sabillah
Pharmaciana Vol 10, No 2 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.289 KB) | DOI: 10.12928/pharmaciana.v10i2.16311

Abstract

Zingiber cassumunar Roxb (bengle) is one of the plants with the potential as an immunomodulator. It contain curcumin and some volatile compounds which previously reported to be potential as immunomodulator. This study aims to examine and determine the effect of ethyl acetate fraction of bengle extract on phagocytic activity of macrophages and lymphocyte proliferation in vitro. The bengle rhizome is macerated with 96% etanol solvent and then followed by ethyl acetate fractionation. The macrophage and lymphocyte were used in this study was isolated from male Balb/C strain mice. The macrophage phagocytosis activity was carried out using latex bead assay, and lymphocyte proliferation assay was carried out using MTT assay method. The concentration of extract was used in this study were 25, 50 and 100 μg/mL. The effect of treatment was compared to negative control group.  The results showed that ethyl acetate fraction of bengle extract can increase the phagocytic activity of macrophages was shown by increasing of active phagocytes cells (SFA) and phagocytosis index. The highest percentage of SFA and phagocytic index results were shown at a concentration of 100 μg/mL. The results showed that ethyl acetate fraction of bengle extract could increase lymphocyte proliferation significantly compare to the negative control (p<0.05). This study showed the potential of bengle as immunomodulatory agent.
Co-Authors Dinda Anindya Sabillah Lukman Mahdi Nanik Sulistyani