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AKTIVITAS ANTIFUNGI EKSTRAK ETANOL BATANG BINAHONG (Anredera cordifolia (Tenore) Steen.) TERHADAP Candida albicans SERTA SKRINING FITOKIMIA Kumalasari, Eka; Sulistyani, Nanik
PHARMACIANA Vol 1, No 2: November 2011
Publisher : PHARMACIANA

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Binahong (Anredera cordifolia (Tenore) Steen.) merupakan tanaman yang secara empiris telah digunakan untuk mengobati beberapa penyakit yang disebabkan jamur.Penelitian ini bertujuan untuk mengetahui aktivitas antifungi ekstrak etanol batang Binahong terhadap C. albicans dan mengetahui kandungan kimia dalam ekstrak etanol batang Binahong. Serbuk batang Binahong diekstraksi dengan metode maserasi menggunakan pelarut etanol 70%.Uji antifungi dilakukan dengan metode dilusi cair dengan berbagai konsentrasi ekstrak (85; 86; 87; 88; 89; 90% b/v). Ekstrak dicampur dengan suspensi jamur dalam media CYG dan diinkubasi pada suhu 37ºC selama 18-24 jam untuk menentukan Kadar Hambat Minimum (KHM). Cairan kultur hasil inkubasi digoreskan pada media SDA (Sabouraud Dextrose Agar) untuk menentukan Kadar Bunuh Minimum (KBM). Uji tabung dan kromatografi lapis tipis dilakukan untuk mengetahui golongan senyawa yang terdapat dalam ekstrak etanol batang Binahong. Hasil penelitian menunjukkan bahwa KHM tidak dapat ditentukan karena ekstrak etanol keruh dan berwarna hijau pekat, sedangkan KBM ekstrak etanol batang Binahong terhadap C. albicans adalah 86% b/v. Hasil uji skrining fitokimia dengan uji tabung dan uji kromatografi lapis tipis menunjukkan bahwa batang Binahong mengandung saponin, flavonoid, dan polifenol.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT DAUN BINAHONG (Anredera scandens (L.) Moq.) TERHADAP Shigella flexneri BESERTA PROFIL KROMATOGRAFI LAPIS TIPIS Kusuma Wardhani, Lilies; Sulistyani, Nanik
PHARMACIANA Vol 2, No 1: Mei 2012
Publisher : PHARMACIANA

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Antibakteri adalah senyawa yang dapat digunakan untuk pengobatan infeksi yang disebabkan oleh bakteri. Secara tradisional tanaman Binahong (Anredera scandens (L.) Moq.) dikenal oleh masyarakat untuk mengobati berbagai macam penyakit, di antaranya adalah penyakit infeksi. Tujuan dari penelitian ini adalah mengetahui aktivitas antibakteri ekstrak etil asetat daun Binahong dan Kadar Bunuh Minimum (KBM) terhadap Shigella flexneri, serta untuk mengetahui kandungan kimia yang terdapat dalam ekstrak etil asetat daun Binahong (Anredera scandens(L.) Moq.). Serbuk daun Binahong diekstraksi dengan metode maserasi menggunakan pelarut etil asetat. Uji antibakteri dilakukan dengan metode dilusi cair dengan berbagai konsentrasi ekstrak (8,5%, 8%, 7,5%, 7%, 6,5%, 6%, 5,5%, dan 5% b/v) untuk menentukan KBM. Uji kromatografi lapis tipis dilakukan untuk mengetahui golongan senyawa yang terdapat dalam ekstrak etil asetat daun Binahong. Hasil penelitian ini menunjukkan bahwa KBM ekstrak etil asetat daun Binahong terhadap Shigella flexneri adalah 8 % b/v. Hasil uji skrining fitokimia dengan uji tabung dan kromatografi lapis tipis menunjukkan bahwa ekstrak etil asetat daun Binahong mengandung polifenol, dan saponin.

KERAGAMAN ISOLAT ACTINOMYCETES BERDASARKAN ANALISIS RFLP TERHADAP GEN NRPS Sulistyani, Nanik
PHARMACIANA Vol 3, No 1: Mei 2013
Publisher : PHARMACIANA

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Actinomycetes merupakan salah satu jenis mikroorganisme yang sangat pentingsebagai penghasil senyawa aktif, salah satunya adalah antibiotika. IsolasiActinomycetes dari rizosfer tanaman padi (Oryza sativa L) dan tanaman tin (Ficuscarica L.) telah menghasilkan 13 isolat Actinomycetes penghasil antibiotik. Penelitianini bertujuan mengetahui keragaman isolat berdasarkan analisis RFLP (LestrictionFragment Length Polymorphism) terhadap gen NRPS (Non Ribosomal PeptideSynthetase). Penelitian dilakukan dengan mengisolasi DNA isolat Actinomycetes dandilakukan PCR terhadap gen 16SrRNA dan gen NRPS. Produk PCR terhadap genNRPS selanjutnya dilakukan analisis RFLP menggunakan enzim HaeIII. Identifikasikeberadaan DNA, hasil PCR dan hasil RFLP dilakukan dengan elektroforesis gelagarose. Keragaman isolat dianalisis dengan analisis multivariate. Hasil penelitianmenunjukkan bahwa dari keragaman 13 isolat Actinomycetes dapat dikelompokkanmenjadi 5 kelompok yaitu isolat 5 (kelompok 1), isolat 8 (kelompok 2), isolat 11(kelompok 3), isolat 6,7,9 (kelompok 4) dan isolat 1,2,3,4,10,12,13 (kelompok 5).

AKTIVITAS CAIRAN KULTUR 12 ISOLAT ACTINOMYCETES TERHADAP BAKTERI RESISTEN Mulyadi, .; Sulistyani, Nanik
Jurnal Kesehatan Masyarakat (Journal of Public Health) Vol 7, No 2 (2013): Jurnal Kes Mas FKM UAD September 2013
Publisher : Universitas Ahmad Dahlan

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ABSTRAK Latar Belakang : Munculnya berbagai patogen yang multiresisten memicuÂ pencarian antibiotik baru. Secara historis, Actinomycetes adalah penghasil terbesarÂ antibiotik. Penelitian ini bertujuan untuk menguji aktivitas 12 isolat Actinomycetes terhadap bakteri Staphyllococcus aureus ATCC 25923 dan Eschericia coli 25922Metode : Penelitian dilakukan dengan mengkultur isolat Actinomycetes pada media Starch Nitrate Broth pada suhu kamar dengan penggojokan selama 14 hari. Uji aktivitas cairan kultur dilakukan dengan metode difusi sumuran terhadap S. aureus dan E.Â coli.Hasil : Pertumbuhan bakteri S. aureus dapat dihambat oleh cairan kultur isolat-isolat Actinomycetes yaitu TL, T18, T19, T24, T37, T41, T43, P301, dan P302 berdasarkan munculnyaÂ diameter zone hambatÂ pada pertumbuhan S. aureus.Â Adapun pertumbuhan E. coli dapat dihambat oleh TL, T18, T19, T24, T25, T41, T43, dan P301. Isolat P104 dan T34 tidak menghambat baik terhadap S. aureus maupun E. coli.Kesimpulan :Â Aktivitas antibakteri dihasilkan oleh isolat TL, T18, T19, T24,Â T41, T43Â dan P301 terhadap S. aureus dan E. coli, isolat T37 dan P302 terhadap S. aureus dan isolat T25 terhadap E. coli.Â Â Kata Kunci :Â Actinomycetes, aktivitas, S. aureus, E. coliÂ Â ABSTRACTBackground : The emergence of various multiresistant pathogens to antibiotics stimulate the search of new antibiotics. Historically, actinomycetes are the largest producer of antibiotics. This study aimed to examine the activity of the 12 isolates ofÂ Actinomycetes against Staphylococcus aureus 25923 and Escherichia coli ATCC 25922.Methods : The study was conducted by culturing isolates of Actinomycetes on Starch Nitrate Broth media at room temperature with shaking for 14 days. The activity of the filtrate was tested against bacteria using diffusion method against S. aureus and E.Â coli.Results : The bacterial growth of S. aureus can be inhibited by fluid culture broth of Actinomycetes isolates namely TL, T18, T19, T24, T37, T41, T43, P301, and P302 based on the appearance of the growth inhibition zone diameter of S. aureus. The growth of E. coli can be inhibited by isolates TL, T18, T19, T24, T25, T41, T43, and P301. Isolates P104 and T34 did not inhibit either the S. aureus and E. coli.Conclusion : The antibacterial activity was produced by isolates TL, T18, T19, T24, T41, T43 and P301 against S. aureus and E. coli, by isolates T37 and P302 inhibit only S. aureus as well as by isolate T25 inhibits only E. coli. Â Keywords : Actinomycetes, activity, S. aureus, E. coli

KERAGAMAN ISOLAT ACTINOMYCETES BERDASARKAN ANALISIS RFLP TERHADAP GEN NRPS Sulistyani, Nanik
Pharmaciana Vol 3, No 1 (2013): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Actinomycetes is one of the types of microorganisms that is essential forproducing the active compounds, one of which is an antibiotic. Isolation ofActinomycetes from the rhizosphere of rice plant (Oryza sativa L.) and tin plant (Ficuscarica L.) has yielded 13 isolates of antibiotic-producing Actinomycetes. This studyaims to determine the diversity of isolates by RFLP analysis (Restriction FragmentLength Polymorphism) of the NRPS (Non Ribosomal Peptide Synthetase) gene. Thestudy was carried out by isolating Actinomycetes isolate DNA and performed PCR onthe 16SrRNA and NRPS genes. The RFLP analysis of the NRPS gene PCR products isthen performed using the enzyme HaeIII. Identify the presence of DNA, the PCRproduct and RFLP results was performed by agarose gel electrophoresis. The diversityof isolates was analyzed by multivariate analysis. The results showed that the diversityof 13 isolates of Actinomycetes can be grouped into 5 groups: isolates 5 (group 1),isolates 8 (group 2), isolates 11 (group 3), isolates 6,7,9 (group 4) and isolates1,2,3,4,10,12,13 (group 5).

Uji sitotoksisitas dan antiproliferatif ekstrak etanol daun leunca (Solanum Nigrum,L) terhadap sel raji Dona, Rahma; Sulistyani, Nanik; Nurani, Laela Hayu
Pharmaciana Vol 6, No 2 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Leunca (Solanum nigrum,L.) is one of the medicinal plant which used society for traditional medication as antipyretic, hypotensive and anticancer. The aim ofÂ this research was to know an anticancer activity of ethanol extract of leunca leaves, emphasized on an ability to inhibit the growth Raji cell. Ethanol extract took from leunca leaf powder that epitomized using ethanol solvent by Soxhlet instrument. Citotoxicity test was done by incubating Raji cell at a density of 2x104 with treatment using ethanol extract from leunca (Solanum nigrum L.) leaf in several concentration 500; 250; 125; 62,5; 31,25; 15,62; 7,81 and 3,90 Âµg/ml. A test was done by MTT method and the percent of cell mortality was calculated. The LC50 values were calculated using probit analysis. The research continued with antiproliferation test on treatment sample concentration 50; 25 Âµg/ml with cell control for 24, 48, and 72 hours.The result of research indicate that ethanol extract of leunca leaves had cytotoxic effect towards Raji cell with LC50 values 59,22 Âµg/ml. The result ofÂ antiproliferation test showed that there were the growth of inhibitation on treatment sample with doubling time values 69,56 hour at concentration 50 Âµg/ml; 60,00 hour at concentration 25 Âµg/ml, and doubling time cell control is 44,98 hour.

Chloroform fraction of ethanolic extract of Elephantopus scaber Linn. increase the p53 expression on human breast cancer (T47D) cell line Mahfudh, Nurkhasanah; Sulistyani, Nanik; Mahdi, Lukman
Pharmaciana Vol 7, No 2 (2017): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Elephantopus scaber Linn had been used as traditional medicine. It contain some lacton sesquiterpene with antiproliferative activity. The study aim was to explore the effect of chloroform fraction of ethanolic extract of E.scaber on the expression of p53 on T47D cell line. The ethanol extract was found by maceration method. The extract was then fractionated using petroleum ether and chloroform respectively to get the chloroform fraction. The expression of p53 was observed by immunocytochemistry method with antip53 antibody. The p53 cell line was cultured and treated with fraction with concentration of 7.06 and 3.53 Âµg/ml. The expression of p53 was showed by brown colour in the nucleus of the cell. The result showed that chloroform fraction treatment with concentration of 7.06 Âµg/ml induced apoptotic cell death in the end stage (necrosis). While the treatment with lower concentration 3.53 Âµg/ml could increase the p53 expression.

AKTIVITAS CAIRAN KULTUR 12 ISOLAT ACTINOMYCETES TERHADAP BAKTERI RESISTEN Mulyadi, .; Sulistyani, Nanik
Kes Mas: Jurnal Fakultas Kesehatan Masyarakat Vol 7, No 2 (2013): Kes Mas: Jurnal Fakultas Kesehatan Masyarakat
Publisher : Universitas Ahmad Dahlan

ABSTRAK Latar Belakang : Munculnya berbagai patogen yang multiresisten memicuÂ pencarian antibiotik baru. Secara historis, Actinomycetes adalah penghasil terbesarÂ antibiotik. Penelitian ini bertujuan untuk menguji aktivitas 12 isolat Actinomycetes terhadap bakteri Staphyllococcus aureus ATCC 25923 dan Eschericia coli 25922Metode : Penelitian dilakukan dengan mengkultur isolat Actinomycetes pada media Starch Nitrate Broth pada suhu kamar dengan penggojokan selama 14 hari. Uji aktivitas cairan kultur dilakukan dengan metode difusi sumuran terhadap S. aureus dan E.Â coli.Hasil : Pertumbuhan bakteri S. aureus dapat dihambat oleh cairan kultur isolat-isolat Actinomycetes yaitu TL, T18, T19, T24, T37, T41, T43, P301, dan P302 berdasarkan munculnyaÂ diameter zone hambatÂ pada pertumbuhan S. aureus.Â Adapun pertumbuhan E. coli dapat dihambat oleh TL, T18, T19, T24, T25, T41, T43, dan P301. Isolat P104 dan T34 tidak menghambat baik terhadap S. aureus maupun E. coli.Kesimpulan :Â Aktivitas antibakteri dihasilkan oleh isolat TL, T18, T19, T24,Â T41, T43Â dan P301 terhadap S. aureus dan E. coli, isolat T37 dan P302 terhadap S. aureus dan isolat T25 terhadap E. coli.Â Â Kata Kunci :Â Actinomycetes, aktivitas, S. aureus, E. coliÂ Â ABSTRACTBackground : The emergence of various multiresistant pathogens to antibiotics stimulate the search of new antibiotics. Historically, actinomycetes are the largest producer of antibiotics. This study aimed to examine the activity of the 12 isolates ofÂ Actinomycetes against Staphylococcus aureus 25923 and Escherichia coli ATCC 25922.Methods : The study was conducted by culturing isolates of Actinomycetes on Starch Nitrate Broth media at room temperature with shaking for 14 days. The activity of the filtrate was tested against bacteria using diffusion method against S. aureus and E.Â coli.Results : The bacterial growth of S. aureus can be inhibited by fluid culture broth of Actinomycetes isolates namely TL, T18, T19, T24, T37, T41, T43, P301, and P302 based on the appearance of the growth inhibition zone diameter of S. aureus. The growth of E. coli can be inhibited by isolates TL, T18, T19, T24, T25, T41, T43, and P301. Isolates P104 and T34 did not inhibit either the S. aureus and E. coli.Conclusion : The antibacterial activity was produced by isolates TL, T18, T19, T24, T41, T43 and P301 against S. aureus and E. coli, by isolates T37 and P302 inhibit only S. aureus as well as by isolate T25 inhibits only E. coli. Â Keywords : Actinomycetes, activity, S. aureus, E. coli

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT DAUN BINAHONG (Anredera scandens (L.) Moq.) TERHADAP Shigella flexneri BESERTA PROFIL KROMATOGRAFI LAPIS TIPIS Wardhani, Lilies Kusuma; Sulistyani, Nanik
Pharmaciana Vol 2, No 1 (2012): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Antibacterial compounds that can be used for the treatment of infections causedby bacteria. Traditionally, Anredera scandens is one of plant used as traditionalmedicine. The aims of this research are to find out the antibacterial activity of ethylacetate extract of Anredera scandens leaf and Minimum Bactericidal Concentration(MBC) against Shigella flexneri and to identify the chemical subtances of the ethylacetate extract found in Anredera scandens. The Anredera scandens leaf was extractedby use of maseration method using ethyl acetate as solvent. The antibacterial assay wasdone using liquid dilution method with varied concentration of Anredera scandens(8,5%, 8%, 7,5%, 7%, 6,5%, 6%, 5,5%, dan 5% w/v) to determine MBC.Chromatography test was done to identify the chemical substances of Anrederascandens extract. The result of this research showed that the MBC of Anrederascandens leaf extract was 8% w/v. The result of the phytochemical screening with tubetest and thin layer chromatogram showed that the extract of Anredera scandens leafcontained, polyphenols, and saponin.

AKTIVITAS ANTIFUNGI EKSTRAK ETANOL BATANG BINAHONG (Anredera cordifolia (Tenore) Steen.) TERHADAP Candida albicans SERTA SKRINING FITOKIMIA Kumalasari, Eka; Sulistyani, Nanik
Pharmaciana Vol 1, No 2 (2011): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Anredera cordifolia is a plantused as antifungal empirically. This study aims tofind out the antifungal activity of ethanol extract of A. cordifolia stem against C.albicans and to identify the chemical subtance groups of the ethanol extract of A.cordifolia. The A. cordifolia stem was extracted by maceration method using ethanol 70% as solvent. The antifungal assay was done using liquid dilution method with variousconcentrations of (85; 86; 87; 88; 89; 90% w/v). The mixtures of extract and suspensionof C. albicans in CYG (Casein Yeast Glucose) medium and the media were incubated at37ÂºC for 18-24 hours to determine Minimum Inhibitor Conceration (MIC).Theculturewas streaked on SDA (Sabouraud Dextrose Agar) medium to determineMinimum Fungicidal Conceration (MFC). Chromatographic test and tube test weredone to identify the chemical substances of A. cordifolia extract. The result showed thatthe MIC could not be determined because the extract was brownish and turbid while theMFC of A. cordifolia stem ethanolic extract was 86% w/v. The result of thephytochemical screening with tube test and the thin layer chromatography test showedthat the extract of A. cordifolia stem contained flavonoids, polyphenols, and saponin.

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