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, Kisman

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Author-ID : 299037

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Karakter Morfo-Fisiologi Daun, Penciri Adaptasi Kedelai terhadap Intensitas Cahaya Rendah Kisman, ,; Khumaida, Nurul; Trikoesoemaningtyas, ,; Sobir, ,; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The main objective of this study was to identify the leaf morpho-physiological characters as markers for adaptation of soybean to low light intensity. The field experiment was conducted using split plot design with 3 replications. First factor was soybean genotype consisted of shade tolerant genotypes (Ceneng, Pangrango) and sensitive genotypes (Godek, Slamet). Second factor was low light intensity consisted of: L0 = control (under full sun light), L1 = 5 days under 50% shading (exposed after 21 days after planting/DAP), L2 = 5 days under dark condition (exposed after 21 DAP), L3 = 3 days 50% shading + 5 days sun light (exposed after 18 DAP), dan L4 = 3 days 50% shading + 3 days sun light + 5 day dark conditions (exposed after 15 DAP). The 50% shading was made artificially using black plastic paranet with 50% light transmitted. Results of this study showed that under the conditions of low light intensity, leaf morpho-physiological characters of soybean that could be used as markers for adaptation were leaf area, specific leaf weight, and chlorophyll content. Leaves of shade tolerant genotypes were wider and thinner and also contained higher chlorophyll especially for chlorophyll b than that of shade sensitive genotypes under low light intensity. Key words : Low light intensity, tolerant genotype, leaf area, chlorophyll, specific leaf weight

Pola Pewarisan Adaptasi Kedelai (Glycine max L. Merrill) terhadap Cekaman Naungan Berdasarkan Karakter Morfo-Fisiologi Daun Kisman, ,; Trikoesoemaningtyas, ,; Sobir, ,; Khumaida, Nurul; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 1 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objective of this study was to analyze the inheritance pattern of soybean adaptation to shade stress based on leaf morpho-physiological characters. Genetic materials used in this study consisted of 22 plants of low irradiance (LI)-tolerant genotype (Ceneng) and 22 plants of LI-sensitive genotype (Godek); 21 plants of F1 (Ceneng x Godek); and 114 plants of F2 populations (derived from F1). These populations were planted under shading of paranet 50%. The population was arranged in a Randomized Complete Block Design with two replicates. Analysis of inheritance of soybean adaptation involved estimation of heritability (broad sense) and gene action. Results of this study showed that: adaptation of soybean to low light stress based on characters of yield per plant was highly heritable (68% of broad sense) with partial dominant mode of action. Characters of leaf area and specific leaf weight were highly (68% of broad sense) and moderately (48% of broad sense) heritable, respectively, with additive mode of action. Soybean adaptation based on leaf physiological characters (chlorophyll contents) was highly heritable (70% - 86% of broad sense) in epistatic mode of action. Key words: Soybean, leaf morpho-physiological character, heritability, gene action

Characterization of Full Length Sequence of JJ3 Isolated from Shade Tolerant Soybean Khumaida, Nurul; Kisman, ,; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 2 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The aim of this study was to characterize full length sequence of JJ3 isolated from shade tolerant soybean. The characterization of full length sequence involved analyses of structure of full length sequence, open reading frame (ORF) or coding sequence (CDS), and homology. The analyses of JJ3 full length sequence structure were done using GeneScanW program, coding sequence (CDS) or open reading frame (ORF) using ORF Finder program, and the homology of JJ3 full length sequence were done using BLAST and clustalW programs. Results showed that the full length sequence of JJ3 consisted of single exon and polyA. Single exon was a coding sequence (CDS) 633 bp produced open reading frame (ORF) with 210 amino acid deduction comprised of 136 conserved amino acids and 77 varied amino acids. The conserved domain of JJ3 produced functional protein, PsaD photosystem I (PSI) subunit, related to photosynthetic transport electron in PSI reaction center. The full length of JJ3 homolog to psaD, a gene encoding protein subunit photosystem I (PSI) related to mechanism of plant adaptation to shade stress, in wood tobacco (Nicotiana sylvestris), rice (Oryza sativa), tomato (Lycopersicon esculentum), barley (Hordeum vulgare), spinach (Spinacia oleracea), Arabidopsis (Arabidopsis thaliana), and potato (Solanum tuberosum).Key words: full length sequence, gene, homology, shade tolerant, soybean

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