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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Agromet Forum Pasca Sarjana Jurnal Ilmu Pertanian Jurnal Ilmiah Aplikasi Isotop Radiasi JURNAL SAINS DAN TEKNOLOGI INDONESIA Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Iptek Tanaman Pangan Buletin Plasma Nutfah AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Widyariset Planta Tropika BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Jurnal Agrotek Tropika Jurnal Agrotech Journal of Tropical Crop Science Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) jurnal Ilmu Pertanian (Agricultural Science) JURNAL PANGAN Jurnal Penelitian Tanaman Industri (Littri) Widyariset Makara Journal of Science

Didy Sopandie

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia

Author-ID : 253978

Aerospace Engineering Agriculture, Biological Sciences & Forestry Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Social Sciences Veterinary

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POTENSI BAKTERI ENDOFIT DALAM UPAYA MENINGKATKAN PERTUMBUHAN, PRODUKSI, DAN KANDUNGAN ANDROGRAFOLID PADA TANAMAN SAMBILOTO GUSMAINI, GUSMAINI; AZIZ, SANDRA ARIFIN; MUNIF, ABDUL; SOPANDIE, DIDY; BERMAWIE, NURLIANI
853-8212
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRAKBakteri endofit hidup di dalam jaringan tanaman yang sehat danberperan antara lain di dalam memacu pertumbuhan tanaman denganmenghasilkan senyawa-senyawa zat pengatur tumbuh, seperti IAA, GA 3 ,dan Sitokinin. Tujuan penelitian ini untuk mengevaluasi potensi bakteriendofit dalamÂ meningkatkan pertumbuhan, produksi, dan kadarandrografolid pada tanaman sambiloto. Penelitian dilakukan di rumah kacaBalittro Cimanggu Bogor pada Oktober 2011âMei 2012. Perlakuandisusun mengikuti Rancangan Acak Kelompok, enam perlakuan danempat ulangan. Perlakuan terdiri dari (1) kontrol, dan perlakuan bakteriendofit yaitu (2) 20BB, (3) 5MD, (4) 20BD, (5) 20CD (perlakuan 2-5masing-masing terdiri dari 4 jenis isolat), dan (6) 90AA (isolat tunggal).Suspensi bakteri endofit (50 ml/tanaman) diberikan 4 kali yaitu padaminggu ke 3, 5, 7, dan 9 setelah tanam dengan konsentrasi 10 10 spk/ml.Hasil penelitian menunjukkan bahwa bakteri endofit berpengaruh postifdan nyata dalam meningkatkan pertumbuhan, produksi herba segar dankering serta andrografolid pada tanaman sambiloto lebih baikdibandingkan kontrol. Peningkatan pertumbuhan tertinggi ditunjukkanpada tinggi tanaman dan jumlah cabang primer yaitu masing-masing24,7% (20 CD) dan 42,2% (20 BB). Produksi herba kering meningkat 25-82,81%, sejalan dengan meningkatnya serapan hara N (64,7-158,8%), P(50-100%), dan K (65-155%). Peningkatan produksi herba kering danandrografolid terbaik diperoleh dari penggunaan 20 CD (82,81 dan142,11%), 20 BB (88,75 dan 131,58%), dan 20 BD (65,63 dan 131,58%).Implikasi dari hasil penelitian ini bahwa bakteri endofit berpotensi untukdikembangkan pada budidaya tanaman sambiloto.Kata kunci: Andrographis paniculata, bakteri endofit, andrografolid,pertumbuhan, produksiABSTRACTEndophytic bacteria live within healthy plant tissue and playimportant roles, such as producing compounds of plant growth regulatorssubstances such as IAA, GA 3 , and Cytokinin. The aims of this research isto evaluate the potential of endophytic bacteria to promote the growth,andrographolide content, and dry matter yield of king of bitter. Theresearch was conducted in the greenhouse of Cimanggu Balittro in October2011-May 2012. Treatments were arranged in a randomized completeblock design with six treatments and four replications. Treatments consistof (1) control, and 5 kinds of endophytic bacteria isolates such as (2)20BB, (3) 5MD, (4) 20BD, (5) 20CD (treatments no.2-5, consisted of 4types of isolate), and (6) 90AA (single isolate). The highest presentage ofplant height and number of primary branches were obtained from thetreatment of 20CD (24.7%) and 20BB (42.2%). Increase in the dry herbyield of 25-82.81% was in agreement with increasing in uptake of N (64.7-158.8%), P (50-100%), and K (65-155%). The best treatment with whichyielding high of dry herbs and andrographolide was 20CD isolates (82.81and 142.11%), followed with 20 BB (88.75 and 131.58%), and 20 BD(65.63 and 131.58%). The study implies that endophytic bacteria havepotential for development of king of bitter cultivation.Key words: Andrographis paniculata, endophytic bacteria, androgra-pholide, growth, yield

Comparative Analysis of Rice Transformation Using Agrobacterium tumefaciens and Rhyzobium leguminosarum Rahmawati, Syamsidah; Jefferson, Osmat Azzam; Sopandie, Didy; ., Suharsono; Slamet-Loedin, Inez Hortense
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

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This study was aimed to study the effectiveness of Rhizobium transformation system compared to the most widely used Agrobacterium mediated transformation system on three rice cultivars, Ciherang (Indica), Nipponbare (Japonica), and Rojolele (Javanica). Six day old calli induced from immature embryos were inoculated with Rhizobium leguminosarum bv trifolii ANU845 and Agrobacterium tumefaciens LBA288 that harbored with vector pCAMBIA 5106. This plasmid contained a minimum set of transfer machinery genes and had a gusplus and an hptII gene driven by 35S CaMV promoter in the T-DNA. The results showed that the transformation frequencies (number of PCR positive plants per number of calli inoculated) ranging from 0 to 12.05 % depend on the genotype and transfer agent used. The highest transformation frequency (12.05%) was obtained in Ciherang transformed with R. leguminosarum. Most of the transgenic rice obtainedby Rhizobium transformation were normal in morphology and fertile similar to those obtained by Agrobacterium transformation. Integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis in T0 and T1 generations.Key words : Rhizobium leguminosarum, immature embryos, Agrobacterium tumefaciens

Parameter Genetik dan Seleksi Sorgum [Sorghum bicolor (L.) Moench] Populasi F4 Hasil Single Seed Descent (SSD) Sulistyowati, Yuli; koesoemaningtyas, Tri; Sopandie, Didy; Ardie, Sintho Wahyuning; Nugroho, Satya
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

The objective of this study were to obtain information about genetic parameters of agronomic characters of sorghum populations derived from Single Seed Descent (SSD) method, and to estimate selection differensial and also to select of F4 population that have a high yield and medium plant height . The experiment was carried out at Leuwikopo Farm, Darmaga, Bogor from January to April 2014. The genetic material were F4 population derived from SSD. The results showed that based on the skewness and kurtosis values, all of the agronomy characters observed in this study were poligenic controlled and influenced by additive gene action. The genetic coefficient of variability values for all parameters varied from intermediate to high in F4 population. Agronomic characters in F4 responded positively to selection because of high broad sense heritability estimates. Correlation analysis showed that plant height, number of leaves, stem diameter, panicle length, panicle weight and 100 seed weight have significant and positive correlation to grain yield/plant. Differential value with selection intensity 10 % based on grain yield/plant will increase grain yield/plant 83.89 % and plant height 8.91 % in the next generation, whereas differensial value based on grain yield/plant and plant height will increase grain yield/plants 68.33 % and plant height 0.26 % in the next generation. It means that selected plant were expected increasing yield 83.89 % or 68.33 % in next generation.Keywords: heritability, cefficient genetic variability, correlation analysis, selection differential

PEWARISAN GEN PENANDA HPT(HYGROMYCINE PHOSPHOTRANSFERASE) BERDASARKAN ANALISIS PCR DAN EKSPRESINYA PADA POPULASI PADI TRANSFORMAN MENGOVEREKSPRESIKAN GEN HD ZIP OSHOX-6 [Segregation of hpt Gene by PCR Analysis and its Expression in Transgenic Rice Population Overexpressing HD-Zip oshox6 Gene] Mulyaningsih, Enung Sri; Aswidinnoor, Hajrial; Sopandie, Didy; OuwerkerV, Pieter BF; Slamet Loedin, Inez Hortense
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2051

First generation (TO) transgenic plants do not always segregate their transgenes in a Mendellian segregation pattern. Moreover,instability of heterologous gene expression was often observed in transgenic plants. This phenomenon is often called gene silencing. Gene silencing could happen on different level of gene expression, notably at transcriptional or post-transcriptional level.The purpose of this research was to identify the transgene segregation pattern of a marker gene (hpt) as well as the introduced- regulator gene (OsLEA-oshox6) in second generation (Tl) transgenic rice plants.Gene segregation (hpt) analysis was carried out using PCR method. Gene expression analysis was done by hygromycin antibiotic resistant test of leaf samples.Analysis was carried out on 17 lines of Tl transgenic rice plants from Batutegi and Kasalath cultivars.Mendellian segregation pattern of 3:1 was revealed for all lines based on PCR analysis. Gene expression analysis showed almost all lines was segregated in a Mendellian fashion except for Tl-BT III 2C line. Less transgenic plants that expressed the hpt gene were suggested due to gene silencing effects.It was suggested to happen at transcriptional level.

Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi Sari, Laela; Purwito, Agus; Sopandie, Didy; Purnamaningsih, Ragapadmi; Sudarmonowati, Enny
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6612

The purpose of this study was to identify the selection criteria to obtain a superior mutant derived from the wheat plants of such varieties as Dewata, Selayar and Alibey, adaptive in medium land. The analysis of agronomic growth characters showed a significantly effect on a growth percentage of the initial growth (8 mutants), flowering time (1 mutant), panicle stem length (15 mutants), number of panicles (7 mutants), the number of grains per panicle (8 mutants), grain weight observed (8 mutants), grain weight per genotype (6 mutants), leaf area (2 mutants) and leaf greenness (5 mutants). The effects on the characters of ripe time, harvest, panicle length and plant height were not significant. The mutants of Dewata, Selayar and Alibey could be selected based on the characters of panicle stem length, number of grains per panicle and grain weight per observation because these characters generated more mutants than the other characters. The correlation analysis between the characters of growth and yield components of wheat mutants showed that the number of grains per panicle was positively correlated with the grain weight observed, while the length of panicle stem was positively correlated with grain weight per genotype, number of panicles and leaf area. Hopefully some mutants produced could adapt to the tropical medium land, thus adding to the diversity of wheat germplasm in Indonesia, thereby reducing the import of wheat to Indonesia.How to CiteSari, L., Purwito, A., Sopandie, D., Purnamaningsih, R. & Sudarmonowati, E. (2016). Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi. Biosaintifika: Journal of Biology & Biology Education, 8(3), 353-361.

ADAPTASI TANAMAN Hoya diversifolia BLUME PADA INTENSITAS CAHAYA TINGGI [ ADAPTATION OF Hoya diversifolia BLUME TO HIGH-LIGHT INTENSITY ] Ardie, Sintho Wahyuning; Rahayu, Sri; Susila, Anas D; Sopandie, Didy
AGRITROP Vol 12, No 1 (2014): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Hoya diversifolia Blume merupakan salah satu anggota famili Asclepiadaceae yang potensial dikembangkan sebagai tanaman hias, terutama sebagai dekorasi pergola. Akan tetapi, tanaman ini hidup pada kondisi ternaung di habitat aslinya dan pertumbuhannya pada kondisi cahaya matahari penuh belum diketahui. Oleh karena itu, tujuan dari penelitian ini adalah untuk mempelajari adaptasi tanaman H. diversifolia Bl. pada intensitas cahaya yang berbeda. Penelitian disusun berdasarkan rancangan tersarang, dengan satu faktor dan tiga ulangan. Ulangan tersarang di dalam intensitas cahaya yang terdiri atas tiga taraf, yaitu 28.2 (cahaya penuh), 20.8 (37% naungan) dan 10.1 Klux (64% naungan). Hasil penelitian menunjukkan bahwa tinggi tanaman dan jumlah daun tidak dipengaruhi oleh intensitas cahaya. Tanaman yang ditanam pada kondisi cahaya penuh memiliki jumlah buku lebih sedikit, daun yang lebih tipis, dan warna daun yang kekuningan. Hasil penelitian ini mengindikasikan bahwa H. diversifolia Bl. dapat ditanam pada kondisi naungan hingga ternaung sebagian, dan menanam pada kondisi cahaya matahari penuh tidak direkomendasikan. Kata kunci: Asclepiadaceae, intensitas cahaya, naungan, tanaman hias

Aplikasi Konsorsium Mikrob Filosfer dan Rizosfer Untuk Meningkatkan Pertumbuhan dan Hasil Tanaman Padi Application of Phyllosphere and Rhizosphere Microbial Consortium to Improve Rice Growth and Production Aksarah Pas, Aris; Sopandie, Didy; Trikoesoemaningtyas, Trikoesoemaningtyas; Santosa, Dwi Andreas Santosa
JURNAL PANGAN Vol 24, No 1 (2015): PANGAN
Publisher : Perum BULOG

Pendekatan secara biologi, memanfaatkan konsorsium mikrob filosfer dan mikrob rizosfer merupakan langkah alternatif mengurangi dampak negatif penggunaan pupuk sintetik, untuk mencapai produksi padi yang tinggi. Penelitian ini bertujuan untuk mempelajari peran konsorsium mikrob filosfer dan mikrob rizosfer terhadap pertumbuhan dan hasil tanaman padi. Penelitian dilakukan mulai bulan Januari hingga Mei 2014, di Green House Indonesian Centre For Biodiversity and Biotecnology, Bogor. Dalam penelitian ini digunakan konsorsium mikrob terbaik hasil seleksi, yaitu konsorsium mikrob filosfer Fm48 dari daun tumbuhan Emmerrilia ovalis Miq Dandy dan konsorsium mikrob rizosfer R15 dari rizosfer tumbuhan Physalis angulata L. Penelitian menggunakan Rancangan Acak Kelompok (RAK) satu faktor, yang terdiri atas tiga perlakuan, yaitu : pemberian pupuk N sintetik setengah dosis anjuran yaitu 30 N/ha, pemberian pupuk N sintetik sesuai dosis anjuran, yaitu 60 kg N/ha dan pemberian kombinasi konsorsium mikrob filosfer Fm48 dan mikrob rizosfer R15 dengan diberi pupuk N sintetik setengah dosis anjuran. Hasil penelitian menunjukkan bahwa, aplikasi kombinasi konsorsium mikrob dengan pemberian pupuk N sintetik setengah dosis anjuran, meningkatkan jumlah anakan, bobot kering tanaman, jumlah anakan produktif dan bobot segar malai setara dengan pemberian pupuk N sintetik sesuai dosis anjuran. Biological approach, by utilizing phyllosphere and rhizosphere microbial consortium, offers n alternative method to avoid the negative impact of synthetic fertilizer to the environment to achieve higher rice production. This research aims to study the role of phyllosphere and rhizosphere microbial consortium on the growth and yield of rice plants. The study was conducted from January to May 2014 in Green House Indonesian Centre For Biodiversity and Biotechnology, Bogor. Microbial consortium of phyllosphere Fm48 from plant leaves Emmerrilia ovalis Miq Dandy and microbial consortium of rhizosphere R15 from plant rhizosphere Physalis angulata L. are selected for this study. This research is designed in randomized block design with one factor, which consists of three treatments, namely: half recommended dose of synthetic N fertilizer (30 kg N/ha), recommended dose of Synthetic N fertilizer (60 kg N/ha), and a combination of microbial consortium of phyllosphere and rhizosphere microbes plus half recommended dose of synthetic N fertilizer. The results show that the applications of microbial consortium combined with half of recommended dose of synthetic N fertilizer, increases the number of tillers, plant dry weight, number of productive tillers, and panicles fresh weight equivalent to the use of recommended dosage of synthetic N fertilizer.

Pengaruh Kedalaman Muka Air dan Amelioran terhadap Produktivitas Kedelai di Lahan Sulfat Masam Effects of Water Depth and Ameliorant to Soybean Productivity on Acid Sulphate Soil Ilona Noyaa, Alce; Ghulamahdi, Munif; Sopandie, Didy; Sutandi, Atang; Melati, Maya
JURNAL PANGAN Vol 23, No 2 (2014): PANGAN
Publisher : Perum BULOG

Oksidasi pirit di lahan sulfat masam menyebabkan pH tanah turun sehingga meningkatkan kelarutan aluminium dan besi. Budidaya jenuh air mempertahankan kedalaman muka air tanah dan membuat lapisan di bawahnya jenuh. Penelitian bertujuan menentukan pengaruh kedalaman muka air tanah dan ameliorasi terhadap beberapa sifat kimia tanah dan produktivitas kedelai. Penelitian dilaksanakan di lahan sulfat masam Desa Banyu Urip Kecamatan Tanjung Lago Kabupaten Banyuasin, Propinsi Sumatera Selatan, sejak Juni sampai Oktober 2012. Penelitian menggunakan rancangan petak-petak terpisahdengan 3 ulangan. Faktor utama adalah tinggi air dalam parit 10 cm dan 20 cm di bawah permukaan tanah dengan pembanding budidaya kering. Faktor kedua adalah amelioran : tanpa amelioran, kapur dan abu jerami. Faktor ketiga adalah genotipe Anjasmoro, Yellow Biloxi, Tanggamus dan Lawit. Hasil penelitian menunjukkan interaksi kedalaman muka air dan ameliorasi meningkatkan pH tanah menjadi 4,67; mempertahankan kadar P tanah 10,70 ppm; meningkatkan kadar K, Ca dan Mg tanah menjadi 1,15; 11,70 dan 6,90 me/100 g. Kadar Fe tanah turun menjadi 12,14 ppm sedangkan kadar Al dan kejenuhan Alturun menjadi 2,06 ppm dan 10,36 persen. Tanggamus memiliki produktivitas tertinggi (2,47 t/ha) karena memiliki lebih banyak jumlah daun (31,5), jumlah cabang (4,5 - 5,3), jumlah buku produktif (27,67) dan jumlah polong isi (80,9).Pyrite oxidation causes the soil pH drops, thus increasing the solubility of aluminium and iron. Saturated soil culture maintains the water depth and makes the soil below saturated. This study aims to determine the effects of soil water depth and amelioration on soil chemical properties and soybean productivity. The experiment is conducted on acid sulphate soil Banyu Urip, South Sumatera Province, from June until October 2012. The experiment is arranged in a split split plot design with three replications. The main factor is water depth in the furrow consisted of 10 and 20 cm under soil surface. The second factor is ameliorant: without ameliorant, lime and straw ash. The third factors are genotypes: Anjasmoro, Yellow Biloxi, Tanggamus and Lawit. The results show that interaction of water depth and amelioration increase soil pH to 4.67, maintain soil P at 10.70 ppm and increase soil K, Ca and Mg to 1.15, 11.70 and 6.90 me/100 g. The soil Fe decrease to 12.14 ppm, whereas Al and Al saturated decrease to 2.06 ppm and 10.36 percent, respectively. Tanggamus has the highest productivity (2.47 t/ha), supported by higher number of leaves (31.5), branches (4.5 - 5.3), productive nodes (27.67) and filled pods (80.9.)

Peningkatan Produktivitas Tanaman Padi (Oryza sativa L.) melalui Pemberian Nano Silika Increased Productivity of Rice Plants (Oryza sativa L.) through The Application of Nano Silica Sugianta, Sugianta; Junaedi, Ahmad; Amrullah, Amrullah; Sopandie, Didy
JURNAL PANGAN Vol 23, No 1 (2014): PANGAN
Publisher : Perum BULOG

Beras merupakan salah satu komoditas pangan terpenting di Indonesia karena merupakan makanan pokok sebagian besar penduduk Indonesia. Namun sampai saat ini produktivitas tanaman padi masih rendah dan belum dapat memenuhi kebutuhan secara keseluruhan. Oleh karena itu, perlu upaya nyata untuk meningkatkan hasil panen tanaman padi. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian unsur hara silika (Si) dalam ukuran nano yang diisolasi dari sekam padi terhadap pertumbuhan, respon morfologi dan fisiologi serta produktivitas tanaman padi sawah. Perlakuan yang diberikan terdiri atas pemberian pupuk SiP 300 kg/ha (S2), pemberian nano silika koloid 10 ppm (S3), 20 ppm (S4), 30 ppm (S5) dan kontrol/tanpa silika (S1). Berdasarkan hasil penelitian ini disimpulkan bahwa pemberian nano silika koloid 20 ppm dan 30 ppm secara umum memberikan pengaruh yang terbaik pada pertumbuhan, respon morfologi, fisiologi dan produktivitas tanaman padi kecuali pada jumlah stomata.Rice is one of the most important staple food commodities in Indonesia. So far, however, the productivity of rice is still low and has not been able to meet the overall domestic needs. Therefore, a real effort to improve the harvest rice crops is urgently needed. This research aims to investigate the influence of silica (Si) nutrient elements, to be applied in nano size isolated from rice husk, on the growth, morphology and physiology responses as well as the productivity of the wet land rice. The treatment consists of the application of fertilizer SiP 300 kg/ha (S2), the colloid nano silica 10 ppm (S3), 20 ppm (S4), 30 ppm (S5) and kontrol/with no silica (S1). The results showed that the application of colloid nano silica 20 ppm and 30 ppm generally resulted in the best growth, morphological, physiological responses and productivity of the rice plant except for the number of stomata.

Cloning and Characterization of Partial Chlorophyll a Oxygenase (CAO) Gene Involved in Soybean Shade Tolerance Mechanism Khumaida, Nurul; Kisman, Kisman; Sopandie, Didy
Journal of Tropical Crop Science Vol 2 No 2 (2015): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Plant tolerance mechanism under shade stress is indicated by high chlorophyll-b content, which is synthesized from chlorophyll a by chlorophyll a-oxygenase (CAO) gene. The study was aimed to clone and characterize the partial of CAO gene involved in shade tolerance mechanism in soybean. Total RNA was isolated from the second trifoliate leaves of soybean plants and the first strand cDNA was prepared using Reverse Transcriptase Moloney Murine Leukemia Virus (RT-M-MLV) (RNase H-). Northern blot hybridization was used to analyze expression of partial CAO gene. The result showed that one partial gene, CAO 3-4 (1,052 bp), comprised of 292 adenine (a), 241 cytosine (c), 276 guanine (g) and 242 thymine (t), therefore the number of amino acid deduction was 338. The expression of CAO 3-4 partial gene was higher in shade tolerant genotypes than in shade sensitive genotypes, both under 50% shade and dark. These results demonstrated that the CAO gene is involved in shade tolerance mechanism of soybean.

Co-Authors , Hamin , Kisman , Purwono , Suharsono , Syafruddin , Tasliah . SUHARSONO . SUWARNO A. HAITAMI A.A. Ketut Agung Cahyawan W Abd. Aziz Syarif ABDUL KARIM MAKARIM Abdul Munif Abdul Qadir Adolf Pieter Lontoh, Adolf Pieter Agus Purwito AHMAD JUNAEDI AKIHO YOKOTA AKIHO YOKOTA Akiho Yokota Aksarah Pas, Aris Alce Ilona Noyaa Alifiya Herwitarahman Amris Makmur Amrullah Amrullah Anas D Susila Anas Dinurrohman Susila ANGELITA PUJI LESTARI Angelita Puji Lestari Annisa Fadila Ardie, and Sintho Wahyuning Ardie, dan Sintho Wahyuning Arvita Netti Sihaloho, Arvita Netti ASMARLAILI SAHAR Atang Sutandi Azri Kusuma Dewi Azri Kusuma Dewi Budi Mulyanto Chairani Hanum Chairani Hanum Christoph Leuschner Deden Derajat Matra Desta Wirnas Dhika Prita Hapsari DWI ANDREAS SANTOSA Edi Santosa Edi Santosa Edi Santosa Efendi, Darda Enny Sudarmonowati Enny Sudarmonowati Enung Sri Mulyaningsih Enung Sri Mulyaningsih Eny Widajati Erma Prihastanti Fadliah Salim Faqih Udin Gusmaini Gusmaini GUSMAINI GUSMAINI GUSMAINI GUSMAINI, GUSMAINI HAJRIAL ASWIDINNOOR Hajrial Aswidinoor Halimatus Syahdia Hasibuan Hamim Hamim Hanedi Darmasetiawan HANNA ARTUTI EKAMAWANTI Hapsoh Hidayah, Agus Nur I Ketut Suada IBNUL QAYIM Ida Hanarida Soemantri Ika Mariska Ika Mariska Ika Mariska Ilona Noyaa, Alce Imam Widodo Inez Hortense Slamet Loedin Inez Hortense Slamet Loedin Inez Hortense Slamet-Loedin INEZ HORTENSE SLAMET-LOEDIN Iskandar Lubis Joko Prasetiyono Joko Prasetiyono Juang Gema Kartika K. Idris Karlin Agustina Kartika Ning Tyas Ketty Suketi KINYA AKASHI KINYA AKASHI Kisman Kisman Komaruddin Idris Komaruddin Idris La Muhuria Laela Sari Laela Sari laela Sari, laela Lukman Chakim Luluk Setyaningsih Lutfy Ditya Cahyanti, Lutfy Ditya M A Chozin M. A. Chozin Maemonah, Maemonah Mahpuzah, Marfiatun Mariska, Ika Masdiar Bustamam Masdiar Bustamam Masumi Moritsugu Matra, Deden Derajat Maya Melati Mayang Sari Meliala, Merry Gloria Moh. Yani Momongan, Jorex Daniel Muhamad Syukur Muhammad Arif Yudiarto Muhammad Jusuf Munarti Munif Ghulamahdi Muzuni, Muzuni Nani Heryani Nasution, Annisa Andrini Ni Made Armini Wiendi Nurliani Bermawie Nurliani Bermawie NURLIANI BERMAWIE Nurul Khumaida Osmat Azzam Jefferson Osmat Azzam Jefferson, Osmat Azzam OuwerkerV, Pieter BF Pieter B.F. Ouwerkerk Pieter BF OuwerkerV Putri Andini Mandasari RADITE TISTAMA Ragapadmi Purnamaningsih RAGAPADMI PURNAMANINGSIH Ratna Yuniati Rini, Erin Puspita Roedhy Poerwanto Rossa Yunita Rossa Yunita Rossa Yunita Rosyad, Astryani S. Suharsono Saepudin, Adam Sandi, Yusri Iryas Sandra Arifin Aziz Saragih, Ery Leonardo Sari, Laela Sarsidi Sastrosumarjo SATRIYAS ILYAS Satya Nugroho Sinaga, Falencia Sinaga, Parlin H. Sintho Wahyuning Ardie Siti Marwiyah Slamet Loedin, Inez Hortense Sobir Sobir SOEKISMAN TJITROSEMITO Soeranto Human Sri Mulatsih SRI RAHAYU Sri Setyati Harjadi Sri Wilarso Budi Sudarmonowati, Enny Sudirman Yahya Sudirman Yahya Sugianta Sugianta Sugianta, Sugianta Sugiono Moeljopawiro SUGIONO MOELJOPAWIRO Suharsono . Suharsono Suharsono Suharsono Suharsono Suhartanto, Muhammad Rahmad Sumiati, dan Sungkono Sungkono Supijatno Surjono Hadi Sutjahjo Suwarno Suwarno Suwarno Suwarno Suwarto Suwarto Syamsidah Rahmawati Syamsidah Rahmawati, Syamsidah T. Kawasaki T. M. H. Oelim Tanari, Yulinda Tintin Suhartini Titin Handayani Toshio Kawasaki Toyip Toyip TRI KOESOEMANINGTYAS Tri Lestari Trikoesoemaningtyas Utami, Nita Nur UTUT WIDYASTUTI UTUT WIDYASTUTI Utut Widyastuti Utut Widyastuti Suharsono Wage Ratna Rohaeni Wahju Q. Mugnisjah Wahju Qamara Mugnisjah Yadi Setiadi Yadi Setiadi Yadi Setiadi YADI SETIADI Yonny Koesmaryono Yopie Moelyohadi Yuli Sulistyowati YULIN LESTARI Yuniati, Ratna Yunita, Rossa

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