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All Journal Journal of the Indonesian Tropical Animal Agriculture Tropical Animal Science Journal
D. Kisworo
Faculty of Animal Science, Mataram University, Jl. Majapahit No. 62 Mataram NTB 83125
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Energy

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DEVELOPMENT OF ENZYME-LINKAGE IMMUNOSORBENT ASSAY AGAINST TYPE B OF CLOSTRIDIUM BOTULINUM: A PRELIMINARY STUDY Depamede, S.N.; Kisworo, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 4 (2011): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.4.237-242

Abstract

Clostridium botulinum neurotoxin (BoNTs) is one of the causes of economic loss in the livestockindustry. This economic loss would be as a direct result when animals poisoned by BoNTs or indirectlywhen the livestock products are contaminated by BoNTs, which end up with the products are banned byauthority. Therefore a routine surveillance of BoNTs in the farm and in livestock product processingindustry is urgently needed. One of the most relatively quick and accurate methods to perform a routinedetection of the presence of BoNTs is enzyme-linkage immunosorbant assay (ELISA). In this article wedescribe the results of the development of ELISA, using polyclonal antibodies against BoNTs-Bproduced locally. Antibodies were generated from six Balb/c mice with standard immunologicalmethods. Mice were immunized three times for a period of 8 weeks with a commercial type BClostridium botulinum toxoid at a dose of 100 ng per mouse per injection. The resulting antibody waspurified by a combination of ammonium sulfate precipitation 50% (w/v) technique and a protein Acolumn method. The results of this preliminary study indicated that the developed ELISA methodcapable of detecting type B Clostridium botulinum toxin up to 1.0 ng/ml.
Evaluating Physicochemical Properties of Whey-Chia Seed Edible Films for Biodegradable Packaging Fahrullah, F.; Noersidiq, A.; Kisworo, D.; Maruddin, F.
Tropical Animal Science Journal Vol. 47 No. 4 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.4.519

Abstract

The use of whey-chia seed edible films can help reduce environmental pollution while preserving the quality of food products. Films were produced using varying ratios of whey to chia seed (v/w) (whey-chia seed ratio of 1:0.5 (W1), 1:0.75 (W2), and 1:1 (W3)) through a completely randomized design with three treatments and six replications. The results showed significant differences (p<0.01) in elongation, tensile strength, moisture content, solubility, and color properties, except for crude fiber content (p>0.05). As the whey:chia seed ratio increased to 1:1, elongation increased up to 76.77%, while tensile strength decreased to 3.876 MPa, indicating an inverse relationship between these properties. The film with a whey:chia seed ratio of 1:0.5 showed 71.08% elongation but higher tensile strength (4.306 MPa) compared to the W3 treatment. The whey:chia seed ratio of 1:1 chia seed film also had the highest moisture content (49.52%), solubility (53.69%), and fiber content (15.67%). Increasing the ratio of chia seed resulted in a brighter and more transparent appearance. The microstructure of the film was continuous, compact, and homogeneous, without any irregularities such as cracks or air bubbles. The study concluded that chia seeds enhance the physicochemical and mechanical properties of whey-based edible films, with the optimal film produced at a 1:1 whey-chia seed ratio.
Co-Authors Fahrullah Fahrullah Maruddin, F. Noersidiq, A. Sulaiman Ngongu Depamede