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All Journal Jurnal Sain Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Sains Teknologi dan Lingkungan (JSTL) YARSI Medical Journal Jurnal Gema Ngabdi Jurnal Abdi Insani
Sulaiman Ngongu Depamede
Fakultas Peternakan Universitas Mataram
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Imunobiologi Sel Sertoli: Prospek Pemanfaatan Sel Sertoli bagi Alternatif Penanganan Cangkok Jaringan N Depamede, Sulaiman
Jurnal Kedokteran YARSI Vol 18, No 2 (2010): MEI - AGUSTUS 2010
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.261 KB) | DOI: 10.33476/jky.v18i2.190

Abstract

Peran utama sel Sertoli adalah merawat dan mengatur perkembangan spermatozoa di dalam testis. Peran ini dilakukan secara anatomis fisiologis dengan membentuk blood testis barrier dan dengan mensekresikan beberapa faktor seperti Fas ligand dan transforming growth factors. Faktor-faktor tersebut berperan bagi terciptanya kondisi imunologis khusus di testis sehingga spermatozoa terlindung dari serangan sistem autoimun tubuh. Beberapa dekade terakhir, penelitian difokuskan pada upaya pemanfaatan sel Sertoli di luar habitat aslinya, sebagai imunosupresan alami dalam penanganan cangkok jaringan. Dalam tulisan ini dibahas beberapa upaya tersebut, dengan beberapa contoh kombinasi cangkok pankreas dan sel Sertoli dalam upaya menangani masalah diabetes melitus. Hasil-hasil penelitian menunjukkan ko-transplantasi sel Sertoli dan sel pankreas berdampak positif bagi perpanjangan usia cangkokan hingga 100 hari dibanding kontrol dengan efek positif bagi proses normoglycemic hewan coba. Di Indonesia penelitian tentang upaya pemanfaatan sel Sertoli sebagai salah satu upaya penanganan masalah cangkok jaringan masih terbatas, dengan demikian studi ini perlu dikaji lebih mendalam lagi.
DEVELOPMENT OF ENZYME-LINKAGE IMMUNOSORBENT ASSAY AGAINST TYPE B OF CLOSTRIDIUM BOTULINUM: A PRELIMINARY STUDY Depamede, S.N.; Kisworo, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 4 (2011): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.4.237-242

Abstract

Clostridium botulinum neurotoxin (BoNTs) is one of the causes of economic loss in the livestockindustry. This economic loss would be as a direct result when animals poisoned by BoNTs or indirectlywhen the livestock products are contaminated by BoNTs, which end up with the products are banned byauthority. Therefore a routine surveillance of BoNTs in the farm and in livestock product processingindustry is urgently needed. One of the most relatively quick and accurate methods to perform a routinedetection of the presence of BoNTs is enzyme-linkage immunosorbant assay (ELISA). In this article wedescribe the results of the development of ELISA, using polyclonal antibodies against BoNTs-Bproduced locally. Antibodies were generated from six Balb/c mice with standard immunologicalmethods. Mice were immunized three times for a period of 8 weeks with a commercial type BClostridium botulinum toxoid at a dose of 100 ng per mouse per injection. The resulting antibody waspurified by a combination of ammonium sulfate precipitation 50% (w/v) technique and a protein Acolumn method. The results of this preliminary study indicated that the developed ELISA methodcapable of detecting type B Clostridium botulinum toxin up to 1.0 ng/ml.
UTILIZATION OF POLYCLONAL ANTIBODIES PRODUCED IN LOCAL HORSES (EQUUS CABALLUS) AS A RESOURCE FOR DEVELOPMENT OF ELISA CONJUGATE TO DETECT HEPATITIS B VIRUS (HBV) SURFACE ANTIGENS Depamede, S.N.; Sriasih, M.; Yulianti, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 2 (2012): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.2.103-107

Abstract

The aim of this study was to utilize the antibody produced using Indonesia local horses (Equuscaballus) to make the conjugate of ELISA kit for detection of hepatitis B virus (HBV) surface antigen(HBsAg). The polyclonal antibodies were isolated and purified from local horses immunized repeatedlyusing isolated and purified HBsAg from Indonesia. The antibodies were conjugated with horseradishperoxidase by a modified method of Nakane and Kawaoi. The conjugate activities were performed usingthe principle of ELISA test conducted by the researchers as well as by independent laboratory.Commercial conjugate for HBsAg ELISA was used as a comparison study. The results of this studyindicated that the antibody produced from local horses can be used to make conjugates that werecomparable to commercial HBsAg ELISA kit.
ETEKSI WSSV (WHITE SPOT SYNDROM VIRUS) PADA LOBSTER AIR TAWAR (PROCAMBARUS CLARKII) MENGGUNAKAN METODE REAL TIME-PCR Nefi Andriana; Muhamad Ali; Sulaiman N. Depamede
JURNAL SAINS TEKNOLOGI & LINGKUNGAN Vol. 1 No. 1 (2015): Jurnal Sains Teknologi & Lingkungan
Publisher : LPPM Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.13 KB) | DOI: 10.29303/jstl.v1i1.10

Abstract

Serangan virus White Spot Syndrome (WSSV) telah memberikan dampak finansial yang cukup besar sejak tahun 1992. Tingkat mortalitas yang mencapai 100% menyebabkan banyak pembudidaya udang dan lobster menderita kerugian. Untuk mengatasi permasalahan tersebut sangat diperlukan informasi tentang cara penyebaran virus WSSV sehingga dapat diketahui solusi yang tepat untuk menghentikan penyebarannya pada tambak-tambak udang maupun lobster. Pada penelitian ini, Lobster Air Tawar yang dibudidayakan di BBI Aik Bukak dijadikan kontrol negatif (belum terinfeksi virus WSSV). Hasil penelitian ini menunjukkan bahwa penularan virus WSSV pada lobster air tawar dapat berlangsung melalui konsumsi udang yang telah terinfeksi virus WSSV serta melalui aliran air. Pleopod atau kaki renang yang dideteksi dengan teknologi Real Time Polimerase Chain Reaction (RT-PCR) mengandung virus WSSV yang lebih dominan dibandingkan insangKata kunci: White Spot Syndrome Virus, Lobster Air Tawar, RT-PCR
Tingkat Pengetahuan Publik (Public Knowledge) Terhadap Penyakit Hewan Menular Strategis Rabies Dalam Upaya Mendukung Status Lombok Bebas Rabies Made Sriasih; Sulaiman Ngongu Depamede; Muhammad Ali; Wayan Wariata; Answar Rosyidi
Jurnal Gema Ngabdi Vol. 2 No. 3 (2020): Jurnal Gema Ngabdi
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jgn.v2i3.101

Abstract

Rabies is one of strategic infectious animal diseases that attacks the central nervous system. Rabies is very widespread and is found in nearly 150 countries and regions on all continents, except Antarctica. Although West Nusa Tenggara is one of the nine provinces in Indonesia that is free of rabies, the number of dog bite victims reported on the island of Sumbawa with 32 samples of rabies-carrying animal brains identified as positive in early 2019 indicates the need to increase awareness of rabies transmission to Lombok Island. Increasing public knowledge which includes parents and students at primary school, elementary school and high school about rabies through surveys, counseling and demonstration is one of the efforts to prevent and control rabies. The results showed that the extension activities were able to increase participants' knowledge. High school students had the best level of knowledge (97.5% ± 2.08) when compared to the elementary school participants (93% ± 3.56) and elderly respondents (88.75% ± 4.79) of all indicators of knowledge about rabies. The combination of extension activities with hands-on practice has had a positive effect on increasing public knowledge about rabies
Pengaruh fasciolosis pada sapi bali berdasarkan pemeriksaan darah, serum glutamic pyruvic transaminase (SGPT), dan alkaline phosphatase (ALP) Amirullah Amirullah; Dradjat AS; Sriasih M; Maskur Maskur; Depamede SN; Kisworo D; Kurniasih Kurniasih
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2010.199 KB) | DOI: 10.22146/jsv.42994

Abstract

Investation of Fasciola sp. On several species of cattle in Indonesia has occurred since a long time ago. Fasciolosis caused a high economical loss such as decreasing of carcass and organs, sometimes caused death of calves. The objectives of the research were to evaluate the effect of fasciolosis on Serum Glutamic Pyruvic Transaminase (SGPT), and  the obstruction of  bile duct by Alkaline Phosphatase (ALP) in Bali cattle suffered from fasciolosis. Faeces of Bali cattle raised in endemic areas of fasciolosis were examinated by sedimentation test to identify eggs per gram of faeces (EPG) of Fasciola sp. The blood samples of cattle which positive to fasciolosis were use to study the hematology by autoanalyser, SGPT  and ALP.  The results of research showed that from 89 cattle, 28 were infected by Fasciola sp. whithin of 7-115 EPG (min-max). From 20 fasciolosis cattle were indicated that MCHC of 90%, RBC of 30%, HB of 20%,  and  eosinophils of 2 % of cattle were lower than that of normal standard, while  lymphocytes of 60%, MCV of 40%, ALP of 10%  and SGPT of 5% of cattle were higher than that of normal standard. hematological appearances of cattle suffered from fasciolosis  had macrocytichypochromic anemia, eosinopenia, and lymphocytosis. The increase of SGPT may be caused byhepatic cells destruction by young liver flukes, and the increase of  ALP was caused by the obstruction of bile duct by mature liver flukes. 
Penggunaan Probiotik Dan Magot Untuk Mereduksi Lalat di Peternakan Ayam Pedaging Muara Selayar Desa Pijot Muhamad Ali; Made Sriasih; Anwar Rosyidi; I Wayan Wariata; Muh. Aidil Fitriyan Fadjar Suryadi; Nefi Andriana Fajri; Sulaiman Ngongu Depamede
Jurnal Gema Ngabdi Vol. 4 No. 2 (2022): JURNAL GEMA NGABDI
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jgn.v4i2.253

Abstract

Chicken manure waste is one of the main problems for large-scale broiler cultivation, especially in areas close to residential areas. The accumulation of manure that accumulates under the cage attracts the arrival and development of the flies in the cultivation site. The development of these flies will spread to the houses of residents who live around the cage. As a result, many farmers stop cultivating the broilers because of public complaints. To overcome this problem, the use of probiotics from acetic acid-producing bacteria can be used to reduce the development of flies because of the ability of the bacteria to reduce the pH of the media and create an uncomfortable environment for egg growth. Likewise, the development of maggot can be a competing medium that beats the flay. In community service activities regarding "The use of probiotics and maggot production to reduce flies in the Muara Selayar broiler farm, Pijot Village, Keruak District, East Lombok Regency". Through this activity, the development of flies can be emphasized by the use of probiotics and the development of magot which can be used as chicken feed.
IDENTIFIKASI PENYEBAB KEMATIAN DAN KEKERDILAN PADA AYAM RAS PEDAGING DI KELOMPOK PETERNAK MUARA SELAYAR DESA PIJOT KECAMATAN KERUAK KABUPATEN LOMBOK TIMUR Made Sriasih; Sulaiman Ngongu Depamede; Wayan Wariata; Muhamad Ali; Anwar Rosyidi; Aini Aini
Jurnal Abdi Insani Vol 10 No 2 (2023): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v10i2.488

Abstract

Success in broiler farming depends on several factors, namely feed, day-old chick (DOC), poultry management, and disease attacks. The Muara Selayar broiler farmer group in Pijot village in the Keruak sub-district of East Lombok is one of the non-industrial poultry farmers. The problems faced by the Muara Selayar farmer group are the mortality rate of DOC, which reached 7.4% (above the 5% threshold), and stunting syndrome. This community service activity aimed to identify the causes of the problems faced by the group so that losses experienced by farmers could be minimized. This community service activity was carried out through direct identification of dead chickens (post-mortem identification), surveys, and discussions. Based on the identification results, the cause of death of chickens in the Muara Selayar group was coccidiosis, characterized by reddish feces and bleeding in the cecum. Stunting syndrome in livestock is mainly caused by poor quality (grade) of seeds from the partners. The results of the discussion activities conducted with the farmers showed that they need bargaining power with partners about the quality of the DOC, so it is necessary to have special communication with partners who cooperate with them. Farmers must pay attention to good husbandry practices such as sanitizing flocks and the surrounding environment and treating livestock with safe materials that do not cause drug residues. From this community service activity, it can be concluded that coccidiosis was the cause of the death of chickens in the Muara Selayar farmer group. However, other poultry diseases such as Pullorum, ND, and CRD should receive attention because they often appear during the rearing period based on farmer reports. Stunting syndrome is mainly caused by low DOC quality.
Co-Authors aini aini aini Amirullah Amirullah Answar Rosyidi Anwar Rosyidi D. Kisworo Dradjat AS E. Yulianti Kisworo D Kurniasih Kurniasih M. SRIASIH Made Sriasih Maskur Maskur Muh. Aidil Fitriyan Fadjar Suryadi Muhamad Ali Muhamad Ali Muhammad Ali Nefi Andriana Nefi Andriana Fajri Sriasih M Wariata, Wayan