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Ngain Kristin
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EFFECTIVENESS OF CHROMATOGRAPHIC METHOD FOR ORDERING SECONDARY METABOLITE COMPOUNDS ON PLANT (PEPEROMIA PELLUCIDA L.) WITH METHANOL SOLUTIONS Lutfiana Kartika Dewi; Muhammad Ghufron; Ngain Kristin
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Proceeding 3rd ISET 2017 | International Seminar on Educational Technology 3rd 2017
Publisher : Universitas Muhammadiyah Semarang

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Abstract

This study aims to determine the effectiveness of chromatographic methods to isolate secondary metabolite compounds in methanol condensed extracts of surrogate plants. A total of 200 grams of simplified planting plants were macerated with methanol solvent for 8x24 hours. The resulting maserate is further distilled to produce a methanol condensed extract of 40 grams. Methanol condensed extract was further analyzed by using thin layer chromatography to see how many compounds in the sample. The result of the thin chromatography obtained by the color of the basket showing in the plants there are somecompounds. Keywords: Surety, Isolation, Simplicia, Secondary Metabolite, Flavonoids
EFFECTIVITY METHOD OF CHROMATOGRAPHY TO ISOLATE COMPOUND OF A METABOLITE SECUNDER AT PEPEROMIA PELLUCIDA L. PLANT WITH METHANOL SOLVENT Lutfiana Kartika Dewi; Muhammad Ghufron; Ngain Kristin
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Pendidikan, Sains dan Teknologi
Publisher : Universitas Muhammadiyah Semarang

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Abstract

The aim of this research to identify the efectivity method of chromatography to isolatecompound of a metabolite secunder at Peperomia Pellucida L. plant With methanol solvent. As much as 200 gram simplisia plant macerated with the methanol solvent, n- hexan, and Ethyl acetate during 2x24 hours. Maserat obtained subsequently in distillation to produce condensed of methanol extracts of 40 grams. Extracts of condensed methanol subsequently analyzed using thin layer chromatography to see the existence of compounds in a sample.Results of chromatography thin retrieved the reflection of colors that indicate in plantsecondary metabolite compounds there are messengers.Keywords: Peperomia pellucida L., Isolation, Simplisia, Secondary Metabolites.
Co-Authors Lutfiana Kartika Dewi Muhammad Ghufron