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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia (JHI) AGRIVITA, Journal of Agricultural Science Jurnal Biologi Tropis Buitenzorg: Journal of Tropical Science
Mia Kosmiatin
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jalan Tentara Pelajar 3A, Bogor 16111
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology

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Nucleotide variations of WRKY70 gene sequence related to Huanglongbing resistance in citrus Nugroho, Kristianto; Purwito, Agus; Sukma, Dewi; Kosmiatin, Mia; Santoso, Tri Joko; Reflinur, Reflinur; Mastur, Mastur
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.10092

Abstract

Huanglongbing, inflicted by Candidatus Liberibacter asiaticus in Asia region, is a destructive disease affecting citrus productions worlwide. Several studies have identified resistance genes that play essential roles in the citrus defense system against this pathogen. The goals of this study were to design the specific gene primers from the WRKY70 gene sequence and analyze the nucleotide variations and genetic diversity among several citrus genotypes. Genomic DNA from nine citrus genotypes were amplified using WRKY70-specific gene primers and the products of PCR were sent to Sanger sequencing, while the sequences of the other 12 genotypes were collected from Citrus Genome Database. The results revealed a total of 282 nucleotide variations which consisted of 157 SNPs, 28 insertions, and 97 deletions, were identified in the WRKY70 gene fragment sequence. There were three notable SNPs detected, with only one SNP [C/T] in first intron area at the position of 524 bp downstream from START codon that showed its ability to distinguish between susceptible and tolerant/resistant citrus genotypes. The phylogenetic analysis also revealed the clearly separation among citrus genotypes in two main clusters. The discovery of this SNP is useful for designing a functional marker as a screening tool in citrus breeding program in the future.
Perkecambahan dan Pertumbuhan in vitro Jeruk Nipis (Citrus aurantifolia) Komang Trisna Wirakusuma; Purwito, Agus; Husni, Ali; Kosmiatin, Mia
Jurnal Hortikultura Indonesia (JHI) Vol. 14 No. 1 (2023): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.14.1.1-8

Abstract

In vitro selection to increase the citrus resistance to Huanglongbin-HLB diseases, requires theavailability of lime in vitro shoots as a negative control, because it is one type of citrus that is verysusceptible to HLB. In vitro shoots can be obtained from seed germination in vitro with a more controlledenvironment. Shoots derived from in vitro germination can be used as controls when in vitro artificialinoculation by pathogen suspension is performed to select in vitro breeding lines. This study aims to obtainthe best concentration of gibberellins and scarification treatment for in vitro germination of lime.Germination was carried out on citrus seeds that had been sterilized on the surface of the fruit. Scarificationis done by injuring the testa of the seed. Lime seeds with and without scarification treatment were germinated on MS+VMW base media with the addition of GA3 0 mg L-1, 5 mg L-1, 10 mg L-1, and 15 mgL-1. The results showed that the optimal concentration of GA3 to induce lime seed germination was 5 mgL-1. Scarification by wounding the seed testas significantly accelerated lime germination time. Keywords: Citrus aurantifolia, in vitro selection, negative control, of GA3, scarification
Karakterisasi dan Kelayakan Ekonomi Citrus aurantifolia kultivar ‘Nimas Agrihorti’ sebagai Komoditas Biofarmasi Potensial di Indonesia Budiyati, Emi; Waluyo, Budi; Maghfoer, Moch. Dawam; Kosmiatin, Mia; Triwiratno, Anang; Hanif, Zainuri; Andrini , Anis; Hanim, Faridha; Tina , Siti Agus
Buitenzorg: Journal of Tropical Science Vol 2 No 1 (2025): Vol. 2 No. 1 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i1.17

Abstract

‘Nimas Agrihorti’ is a citrus cultivar developed to fulfil the increasing domestic demand for biopharmaceutical-grade citrus in Indonesia. Its development aligns with the growing public interest in health-promoting agricultural products. This study aimed to evaluate the morphological characteristics, chemical composition, and economic viability of ‘Nimas Agrihorti’ as a biopharmaceutical citrus. Field observations were conducted from June 2019 to October 2020 at the Banjarsari Experimental Field, Bayeman Village, Probolinggo, East Java. Morphological assessments followed the Descriptor List for Citrus by IPGRI, and chemical analyses were performed at the Post-Harvest Laboratory of Brawijaya University, Malang. Economic feasibility was assessed by calculating production costs and revenues over a 2-year cultivation cycle. ‘Nimas Agrihorti’ exhibited favorable morphological traits, including large fruit size (72–82 g), yellow skin, and sweet taste. Chemical analysis revealed vitamin C content of 34.8 mg/100 g and low acidity at 0.45%. The total production cost over 2 years was IDR 42,640,000.00 (equivalent to USD 2,584), with total revenue amounting to IDR 77,360,000.00 (equivalent to USD 4,688), resulting in a Revenue-Cost (R/C) ratio of 2.81. The combination of desirable morphological and chemical characteristics, along with strong economic returns, indicates that ‘Nimas Agrihorti’ holds significant potential for development as a biopharmaceutical citrus cultivar in Indonesia.   Keywords: citrus, cultivar, biopharmaceutical products, economic feasibility
Induksi Embriogenesis Somatik dari Jaringan Endosperma Jeruk Siam (Citrus nobilis Lour.) cv Simadu Kosmiatin, Mia; Purwito, Agus; Wattimena, Gustaff Adolf; Mariska, Ika
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (610.396 KB) | DOI: 10.24831/jai.v42i1.8149

Abstract

ABSTRACTTriploid plants can be obtained from endosperm tissues through somatic embryogenesis regeneration. This research aimed to obtain somatic embryogenesis regeneration technique of tangerine endosperm. There were 3 experiments conducted in this research: 1) Embryogenic callus induction of tangerine endosperm. Endosperms isolated from fruits that were harvested from mother plants 11-13 weeks after anthesis and cultured on Murashige and Skoog (MS) medium by modified vitamin Morel and Wetmore (MW) which treated by 0.1 mg L-1 biotin, 500 mg L-1 malt extract (ME), 500 mg L-1 Casein hydrolisate (CH), 500 mg L-1 ME + 0.1 mg L-1 biotin, and 500 mg L-1CH + 0.1 mg L-1 biotin, 2) Maturation and germination of somatic embryos conducted by embryogenic callus cultured on MS medium by vitamin MW modified with addition of ABA, glutamine, and biotin, and 3) Plantlet elongation conducted on MS medium modified by MW vitamin with addition of GA3 and Kinetin. The best induction medium for embryogenic callus was modified MS enriched with 3 mg L-1 BA and 500 L-1 CH or ME, in succession 84.0 and 80.0%. The best medium for somatic embryos maturation with normal morphological plantlets (54.8%) was modified MS medium without plant growth regulator with higher rate of solidified agent (from 2.5 to 3 g L-1 Phytagel). Plantlets elongation was highly (0.9 cm) occurred on modified MS with enriched of 2.5 mg L-1 GA3. Keywords: Citrus nobilis (Lour.), endosperm culture, in vitro, Simadu tangerine
Transformation of Ponkan Mandarin (Citrus reticulata Blanco) by CRISPR/Cas-9-gRNA-CsCS to increase plant resistance to huanglongbing disease Dian Puji Rahayu; Purwito, Agus; Kosmiatin, Mia; Husni, Ali
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 2 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i2.53465

Abstract

The gene that regulates callose could be modified by CRISPR/Cas9 technology. This research aimed to insert the CRISPR/Cas9-CsCS gene into Ponkan orange genome using Agrobacterium tumefaciens. The explants were soaked in a bacterial suspension for 20 minutes and incubated for 2-3 days. In vitro acceleration growth was conducted with a two-factor completely randomized design. The first factor is the type of explant with three levels (nucellar embryo, zygotic embryo, cotyledon node), and the second factor is the type of media with 6 levels (VMW, MT, MSK0, MSK1, MSK2, MSK3). The results showed of all explant types, the highest plant height average and number of leaves were obtained in a media combination of MS + Kinetin 2 mg L-1, MT, and MS + Kinetin 3 mg L-1. The highest transformation efficiency was in the nucellar embryo explant, while the highest regeneration efficiency was in the zygotic embryo explant. The highest shoot tip grafting percentage was achieved in the cotyledon node explant at 100%. In the grafting phase, the putative transformants before and after artificial bacterial inoculation showed that Ponkan 606 and Ponkan 597 had the highest plant heights, respectively. The intensity of Huanglongbing attacks after bacterial inoculation showed that three genotypes did not show HLB symptoms in the 24th week of observation, namely genotypes 598, 606, and 607. This study concluded that gene transformation in citrus plants produced three genotypes that did not show HLB symptoms. Keywords: transgenic; Agrobacterium tumefaciens; Citrus Vein Phloem Degeneration (CVPD)
Biopharmaceutical potential of indonesian husk tomato (Physalis angulata L.): genetic diversity, metabolite biosynthesis, and breeding for enhanced fruit quality Shoidah, Fajriyatus; Fadillah, Muhammad Asya; Martasari, Chaireni; Nugroho, Kristianto; Wulandari, Dyah Retno; Kosmiatin, Mia; Husni, Ali; Waluyo, Budi; Budiarto, Kurniawan; Yusuf, Hadi Mokhamad
AGRIVITA Journal of Agricultural Science Vol 48, No 3 (2026): October 2026 (IN PRESS)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v48i3.5055

Abstract

The Husk Tomato (Physalis angulata L.) has been valued as a medicinal plant due to its rich secondary metabolites. These compounds include physalins, which exhibit antitumor activity; withanolides, recognized as potent anti-inflammatory agents; and other components such as flavonoids, phenolic acids, alkaloids, and carotenoids. Despite its therapeutic potential, the commercial utilization of P. angulata remains limited by inconsistent fruit quality. This review summarizes current knowledge on Indonesian P. angulata germplasm, focusing on its botany, growth characteristics, genetic diversity, and metabolite profiles. Previous reviews mainly discussed botanical and pharmacological properties, whereas this review emphasizes Indonesian germplasm diversity and breeding perspectives. We critically evaluate several breeding strategies aimed at improving fruit quality, including the utilization of genetic resources, hybridization, mutation breeding, polyploidization, and in vitro techniques. Furthermore, we highlight the application of genetic engineering, particularly the overexpression of the squalene synthase (SQS) gene, suggesting that this approach could efficiently enhance the biosynthesis of squalene derivatives, such as physalins and withanolides. By evaluating the achievements and challenges of these strategies in P. angulata and other Solanaceae species, this review offers a roadmap for future research, aiming to maximize fruit quality and unlock the extensive biopharmaceutical applications of the Indonesian Husk Tomato.
Co-Authors Agus Purwito Ali Husni Andrini , Anis Baroya, Mushlihatun Budi Waluyo Chaireni Martasari Desyandri Desyandri Dewi Sukma Dian Puji Rahayu Emi Budiyati, Emi Fadillah, Muhammad Asya fatimah Fatimah Gustaff Adolf Wattimena Hanif, Zainuri Hanim, Faridha Ika Mariska Komang Trisna Wirakusuma KRISTIANTO NUGROHO, KRISTIANTO Kurniawan Budiarto Mastur Moch. Dawam Maghfoer REFLINUR REFLINUR Shoidah, Fajriyatus Tina , Siti Agus TRI JOKO SANTOSO Triwiratno, Anang Wulandari, Dyah Retno Yusuf, Hadi Mokhamad