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All Journal Jurnal Pertanian Agros Fruitset Sains : Jurnal Pertanian Agroteknologi Journal of Agriculture (JoA) Agroista: Jurnal Agroteknologi
Elisabeth Nanik Kristalisasi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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INDUKSI KALUS EMBRIOGENIK KELAPA SAWIT PADA MEDIA MS DENGAN PENAMBAHAN 2,4-D DAN AIR KELAPA MUDA Titin Setyorini; Elisabeth Nanik Kristalisasi
AGROISTA : Jurnal Agroteknologi Vol. 3 No. 1 (2019): MEI
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.897 KB) | DOI: 10.55180/agi.v3i1.50

Abstract

Embryogenic callus produced from somatic cells has a significant role in clonal propagation and oil palm breeding (assembly of superior varieties) through tissue culture techniques. Induction and development of embryogenic callus were determined by media composition, vitamins, amino acids, and growth regulators (hormones). This study aimed to determine the role of natural and synthetic hormone which supplemented in MS medium to induce embryogenic callus of oil palm. The research was conducted in laboratory of plant tissue culture, Stiper Agriculture Institute, Yogyakarta from February to May 2019. The explants were inoculated in solid MS medium with three treatments, namely MS medium (Murashige and Skoog), MS + synthetic hormone (2,4-Diclorophenoxyacetate), and MS + natural hormone (young coconut water). The results showed synthetic hormone (2,4-Diclorophenoxyacetate) which supplemented in MS medium can induce embryogenic callus of oil palm explants. However, natural hormone (coconut water) has not been able to induce embryogenic callus. Keywords: Callus induction, oil palm, 2,4-D, coconut water
APLIKASI MACAM PUPUK ORGANIK (VERMIKOMPOS, ECENG GONDOK, PUPUK KANDANG) DAN MACAM PUPUK P (TSP, ROCK PHOSPATE, SP36) TERHADAP PERTUMBUHAN BIBIT KELAPA SAWIT (Elaeis guineensis Jacq.) DI PRE NURSERY Ramadhan, Agung Fadillah; Syah, Ryan Firman; Kristalisasi, Elisabeth Nanik
Fruitset Sains : Jurnal Pertanian Agroteknologi Vol. 13 No. 1 (2025): April: Ilmu Pertanian dan Bidang Terkait
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/fruitset.v13i1.6184

Abstract

Palm oil is a commodity of plantation plantations that produce vegetable oils that are very instrumental in various industries. To increase good production of palm oil must be maintained. Actions are carried out from planting to the end of the harvest. Fertilization is in the form of its actions. This lesson wants to explore each contribution from the aspects that have been determined to be the core of the problem topic that we want to raise. The lesson was held in the designated community area from October 2024- January 2025. The lesson was through a designed trial (RAL) from some aspects. The initial aspect of the type of organic fertilizer which includes 4 levels: Vermicompost, Control, Water Hyacinth Compost & Cow Manure. From the second aspect of P fertilizer which includes some: SP36, Rock Phosphate & TSP. Then 12 combined actions were found from the 4 x 12 = 48 plant trials. This lesson shows that there is no real relationship between P and Organic actions on Seedling Development. Water hyacinth compost given 250g/polybag contributes to the stem and height of the seedlings. Giving P at a dose of 1.5g/polybag contributes to the development of early nursery sekeywords are goodedlings.
Solanum Melongena L. Reaction to Combining Doses of PGPR Imperata Cylindrica and Cow Manure: Growth and Yield Nareswari, Daniela Hapsari; Kristalisasi, Elisabeth Nanik; Ardiani, Fani
Journal of Agriculture Vol. 4 No. 02 (2025): Research Articles July 2025
Publisher : ITScience (Information Technology and Science)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47709/joa.v4i02.6663

Abstract

This study aims to examine the interaction between PGPR roots of Imperata cylindrica and cow manure on the growth and yield of Solanum melongena L., and to determine the optimal dose of both treatments. The study used a factorial Completely Randomized Design (CRD) with two factors: the dose of PGPR roots of Imperata cylindrica (0, 10, 30, 50 ml) and cow manure (0, 1, 2 kg), with five replications, the data were analyzed by ANOVA at a 5% level and further tested with DMRT at a 5% level. The results showed a significant interaction between PGPR roots of Imperata cylindrica and cow manure on plant height and number of leaves, with the best results being PGPR 30 ml and cow manure 2 kg. The dose of PGPR Imperata cylindrica roots of 30 ml can increase plant height, number of leaves, fruit weight, fresh root weight, and root length. However, the dose of PGPR Imperata cylindrica roots of 50 ml can reduce the percentage of fruit wilting. The dose of 2 kg of cow manure can increase plant height, number of leaves, number of fruits, fruit weight, fresh root weight, dry root weight, and root length of plants. This study concluded that PGPR Imperata cylindrica roots and cow manure or their combination affect the growth and production of Solanum melongena L. plants.
PENGARUH DOSIS PGPR DAN LCPKS TERHADAP PERTUMBUHAN BIBIT KELAPA SAWIT DI PEMBIBITAN AWAL Kristalisasi, Elisabeth Nanik; Rusmarini, Umi Kusumastuti; Perwana, Rifaldi Gravian
Agros Journal of Agriculture Science Vol 24, No 2 (2022): edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v24i2.1949

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh dosis PGPR dan LCPKS terhadap pertumbuhan bibit kelapa sawit di pembibitan awal. Penelitian dilakukan di PT. Perkebunan Nusantara III yang terletak di desa Sei Meranti, Kecamatan Torgamba, Kabupaten Labuhan Batu Selatan, Sumatera Utara, pada bulan Juni sampai September 2021. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) yang terdiri dari dua faktor. Faktor pertama adalah dosis PGPR terdiri dari 3 aras (30, 40, dan 50 ml/bibit). Faktor kedua adalah dosis LCPKS terdiri dari 4 aras (0/kontrol, 30, 40, dan 50 ml/bibit).  Hasil penelitian menunjukkan ada interaksi nyata antara aplikasi dosis PGPR dan LCPKS terhadap tinggi bibit, diameter batang, bobot kering akar, bobot segar tajuk, bobot kering tajuk, volume akar, bobot kering tanaman, dan bobot segar tanaman kelapa sawit di pembibitan awal. Kombinasi perlakuan terbaik adalah PGPR 50 ml/bibit dengan LCPKS 50 ml/bibit. Dosis PGPR maupun LCPKS memberikan pengaruh  sama terhadap parameter panjang akar serta bobot segar akar.
Isolasi dan Uji Potensi Bakteri Pelarut Fosfat dari Rizosfer Tanaman Kopi Robusta (Coffea robusta) Herlambang, Hari Inti; Himawan, Achmad; Kristalisasi, Elisabeth Nanik
AGROISTA : Jurnal Agroteknologi Vol. 9 No. 1 (2025): MEI
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55180/agi.v9i1.1420

Abstract

Nutrient elements are very important for robusta coffee plants, but the availability of these nutrients in acidic soil (pH less than 5.5) or alkaline soil (pH greater than 8) is limited, as phosphorus (P) becomes bound by iron (Fe), aluminum (Al), and calcium (Ca), making it unavailable to plants. Therefore, phosphate-solubilizing bacteria are needed to convert unavailable phosphorus into available phosphorus. The purpose of this study was to identify the presence of phosphate-solubilizing bacteria in the rhizosphere of robusta coffee plants from Glagaharjo Village, Kepuharjo Village, and Umbulharjo Village, as well as to isolate and test their potential as phosphate-solubilizing bacteria. The method used in the study was an observational method presented in descriptive form. The descriptive writing includes information about the sampling location (temperature, humidity, sunlight intensity, altitude), procedures for isolating phosphate-solubilizing bacteria, macroscopic observations, microscopic observations, phosphate solubilization potential testing, catalase testing, and oxidase testing. The results of the study found 45 bacterial isolates from the three Villages. However, analysis of phosphate-solubilizing bacteria showed that only 16 isolates were suspected to be phosphate-solubilizing bacteria, potentially capable of solubilizing phosphate, as indicated by clear zones formed when cultured on Pikovskaya agar medium. Based on the characterization results, phosphate-solubilizing bacteria were found in each of the sample locations: Location 1 (Glagaharjo Village), Location 2 (Kepuharjo Village), and Location 3 (Kepuharjo Village, Cangkringan Subdistrict, Sleman Regency). The identified bacterial genera were Azotobacter and Pseudomonas, and all isolates tested positive in catalase and oxidase tests.
Co-Authors Fani Ardiani Herlambang, Hari Inti Himawan, Achmad Nareswari, Daniela Hapsari Perwana, Rifaldi Gravian Ramadhan, Agung Fadillah Rusmarini, Umi Kusumastuti Syah, Ryan Firman Titin Setyorini