NOER KUMALA
Departemen of Biochemistry, Faculty of Medice, University of Wijaya Kusuma Surabaya

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The Potency of Protein Extracts from Candida albicansas Bioreceptor on Immunosensor for Diagnosis of Candidiasis ., Masfufatun; Kumala, Noer; Baktir, Afaf
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Currently diagnosis of candidiasis still usingthe traditional standard blood culture method. The traditional method were less sensitive andtime consuming. The purpose of this research were to develope the more sensitive immunosensor based method, and to examine the potency of C. albicans protein extract as bioreceptor to  detect  C. albicans and its biofilm in the blood of candidiasis patients.The research methods include: (1) preparation of  digestive gland liquid of snail (Achatina fulica); (2) extraction of protein from C. albicans  through  enzimatis and mechanic methodsand (3) analyzing the protein extract as bioreseptor through immunodot assay.The research results showed that the snail enzymes has protein content 1.35 mg/ml and  specific activity 1.96 unit/mg. The snail enzyme hydrolyzed  the cell wall of C. albicans  with and without sonication, producedplanktonic extracell protein extract (PEP) and biofilmextracell proteinextract (PEB), planktonic intracell protein extract (PIP), and biofilmintracell protein extract (PIB), withprotein content 1.44; 1.29; 1.29 and 1.21 mg/ml respectively. Thebiofilm intracell protein (BIP) showed antigenic property towardantibody anti-Candida (positive control),giving red spot on imunodot assay. Immunodot assay can distinguishnegative control serum (health man) and positive Candidiasis control by using antigen 1 mg/ml and50ml serum. Keywords: C. albicans, candidiasis, biofilm, immunodot assay
Korelasi Tinggi Badan Terhadap Panjang Os. Femur Dan Os. Carpalia Digiti III Pada Mahasiswa Abu Isa Muhammad Fata Mumtaza; Soekanto, Ayly; Herliana, Olivia; Kumala, Noer
Calvaria Medical Journal Vol 4 No 1 (2026): Edisi Juni 2026
Publisher : Institute for Research and Community Services (LPPM) of University of Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/tx170y17

Abstract

Background: Height is one of the important anthropometric parameters in the fields of anatomy, physical anthropology, and forensic identification. Height estimation can be done by measuring certain body segments because there is a proportional relationship between the length of body segments and height. The length of the femur segment and the carpalia digiti III segment are body parts that have the potential to be used to estimate height. The purpose of this study was to analyze the relationship between height and the length of the femur segment and the length of the carpalia digiti III segment in students of the Faculty of Medicine, Wijaya Kusuma University, Surabaya, Class of 2023. The research method used an analytical observational design with a cross-sectional approach. The study sample consisted of 30 students who met the inclusion criteria. Height was measured using a microtomies, while the length of the femur segment and the carpalia digiti III segment was measured using an anthropometric tape. Data analysis was performed using Spearman's correlation test, Pearson's correlation test, and linear regression. The results showed that the average height of students was 163.48 cm, the average length of the femur segment was 46.61 cm, and the average length of the carpalia digiti III segment was 9.62 cm. There is a very strong and significant relationship between femur segment length and height (r = 0.909; p = 0.000), and a strong and significant relationship between carpalia digiti III segment length and height (r = 0.714; p = 0.000). Regression analysis shows that femur segment length is a more dominant predictor of height than carpalia digiti III segment length. Conclusion: Femur segment length and carpalia digiti III segment length are significantly related to height, and femur segment length has greater potential as a prediction of height in the fields of anthropometry and forensics.