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ANTIBACTERIAL ACTIVITY OF ALOE VERA LEAF INFUSE [Aloe barbadensis Miller] Sulistyani, Nunung; Kurniati, Eni; Yakup, -; Cempaka, Risa Ayu
Jurnal Penelitian Saintek Vol 21, No 2: Oktober 2016
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.923 KB) | DOI: 10.21831/jps.v21i2.13942

Abstract

The aim of this studi is to examine the antibacterial activity of aloe vera leaf infuse (Aloe barbadensis Miller) against human pathogens such as Pseudomonas aeroginosa, Salmonella typhi, Staphylococcus aureus, dan Streptococcus pneumonia. Antibacterial activity test of 20, 40, and 60% aloe vera leaf infuse was using agar well diffusion method. The antibacterial activity is indicated by the formation of a clear zone around the wells. All the concentration of aloe vera leaf infuse (Aloe barbadensis Miller) showed signifi cant antibacterial activity against Pseudomonas aeroginosa, Salmonella typhi, Staphylococcus aureus, but did not show antibacterial activity against Streptococcus pneumonia. Aloe vera leaves infuse at a concentration of 60% can inhibit the growth of Pseudomonas aeroginosa indicated by the formation of a clear zone (16.5 mm), the bacteria Salmonella typhi (34 mm), and Staphylococcus aureus (15 mm). The research recommended aloe vera leaf would be suitable for use as antibacterial agent of disease caused by Pseudomonas aeroginosa, Salmonella typhi, and Staphylococcus aureus. 
Uji Antifungi Susu Fermentasi Komersial pada Candida non-albicans Khikmah, Nur; Sulistyani, Nunung
SCISCITATIO Vol 1 No 1 (2020): Volume 1, Number 1, January 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.11.18

Abstract

Bakteri Asam Laktat (BAL) yang terkandung dalam susu fermentasi akan menghasilkan asam-asam organik, hidrogen peroksida, diasetil, asetaldehid, asetoin, reutinin, reuterisiklin dan bakteriosin, dapat sebagai anti-Candida. Spesies Candida non-albicans seperti C. tropicalis dan C. glabrata sebagai penyebab kandidiasis oral cenderung meningkat. Tujuan penelitian ini mengetahui aktivitas antifungi susu fermentasi komersial pada Candida non-albicans dan viabilitas bakteri asam laktat di dalam susu fermentasi komersial. Aktivitas antifungi pada Candida non-albicans dilakukan dengan metode difusi sumuran. Viabilitas bakteri asam laktat dihitung berdasarkan jumlah bakteri asam laktat sebagai jumlah bakteri total (Total Plate Count). Hasil penelitian menunjukkan bahwa susu fermentasi komersial lebih mampu menghambat C. tropicalis dibandingkan C. glabrata. Viabilitas bakteri asam laktat dalam susu fermentasi komersial 107-1010 CFU/mL.
Efek Rebusan Sereh (Cymbopogon citratus) Terhadap Respon Stress Oksidatif Pada Tikus Wistar Jantan (Rattus norvegicus) Diabetes Widaryanti, Barinta; Khikmah, Nur; Sulistyani, Nunung
Life Science Vol 10 No 2 (2021): November 2021
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v10i2.54457

Abstract

Diabetes mellitus is a metabolic disorder with hyperglycemia. The amount of diabetic patients in Indonesia will increase rapidly without suitable treatment. High level of blood glucose will trigger oxidative stress. The objective of this research wa to investigate the effect of lemongrass on oxidative stress response that was indicated by serum and hepatic Total antioxidant capacity and Malondialdehyde level. Thirty male wistar rat were divided into five groups. K1 was a normal group, K2 was a normal group with lemongrass treatment, K3 was a diabetic group induced by a single dose of Streptozotocin at a concentration of 60 mg/kg body weight, K4 was a diabetic group that was treated with glibenclamid at a concentration of 0.09 mg/200 gr body weight, while K5 was a diabetic group treated with lemongrass at a concentration of 3.6 ml/200 gr body weight. The result showed that lemongrass was able to reduce blood glucose levels, serum MDA and hepatic MDA levels and increased the level of total antioxidant status Diabetes adlah kelainan metabolisme yang ditandai dengan hiperglikemia. Jumlah penderita diabetes mellitus di Indonesia akan mengalami peningkatan jika tidak ada penanganan yang sesuai. Tinggi kadar glukosa darah dapat memicu terjadinya stress oksidatif. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian sereh terhadap respon stress oksidatif yang ditunjukan dengan kapasitas antioksidan total dan kadar malondialdehid. Tigapuluh ekor tikus wistar jantan dibagi menjadi lima kelompok. K1 adalah kelompok normal, K2 adalah kelompok normal dengan perlakuan sereh, K3 adalah kelompok diabetes yang diinduksi dengan streptozotocin 60 mg/kg berat badan single dose, K4 adalah kelompok diabetes yang diberi perlakuan dengan glibenklamid dengan dosis 0.09 mg/200 gr berat badan. Sedangkan K5 adalah kelompok diabetes yang dieri perlakuan dengan rebusan sereh dengan dosis 3.6 ml/200 gr berat badan. Hasil penelitian menunjukan bahwa rebusan sereh mampu menurunkan kadar glukosa darah, konsetrasi malondialdehid serum dan hepar, serta meningkatkan total antioksidan status pada serum dan hepar.
CONTROL OF FLOWER PATHOGEN ACTIVITIES OF SOYBEAN PLANTS IN STRAIN COCONUTION PSEUDOMONAS SP. AND BACILLUS SP. WITH BRADYRHIZOBIUMJAPONICUM Nunung Sulistyani; Aris Tri Wahyudi; Giyanto Giyanto
Jurnal Penelitian Saintek Vol 18, No 1: April 2013
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6665.606 KB) | DOI: 10.21831/jps.v18i1.1835

Abstract

Plant growth promoting rhizobacteria (PGPR) is a bacterial colonization of plant roots that can stimulate the growth of plants directly or indirectly. The PGPR mechanism indirectly results in plant growth as it suppresses the growth of phytopathogen. This study aims to determine the ability of Pseudomonas sp. and Bacillus sp. in controlling the pathogenic root fungal pathogenic fungi. Isolates in this study were Pseudomonas sp. Crb 3, Crb 17, Crb 17i, Crb 68 and Bacillus sp. Cr 24, Cr 44, Cr 66 co-inoculated with B. japonicum in soybean plants in greenhouses. An antagonistic activity test was performed on all isolates and all isolates showed no antagonistic activity. Treatment of combination of strain Pseudomonas sp.Crb 17, Crb 17i and Bacillus sp. Cr 24 inoculated with B. japonicum suppresses the growth of root rot fungi and increases peroxidase. Therefore, Pseudomonas sp. Crb 17, Crb 17i and Bacillus sp. Cr 24 can be used as a biocontrol agent for pathogenic fungi causing plant root disease.
Potensi Beberapa Susu Fermentasi Komersial Sebagai Antifungi Candida albicans Nur Khikmah Nunung Sulistyani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 2, No 1 (2017): February 2017
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v2i1.1505

Abstract

Candida albicans cause opportunistic infection of the oral cavity. Probiotic has health benefits, produces organic acids as anti-C. albicans. The aim of this research was to determine the antifungal activity of commercial fermented milk products against Candida and viability of bacteria in commercial fermented milk products. The antifungal activity was determined using well diffusion method. Viability of lactic acid bacteria was done by plate count method. The results show that commercial fermented milk productshas an activity of anti-C.albicans were shown by irradical zone formed around well containing fermented dairy products. Viability of lactic acid bacteria 107-1010 CFU/mL.
KAPANG DENGAN AKTIVITAS FIBRINOLITIK YANG DIISOLASI DARI TANAH RUMAH POTONG HEWAN Nur Khikmah; Nunung Sulistyani
JBIO: jurnal biosains (the journal of biosciences) Vol 4, No 3 (2018): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v4i3.11354

Abstract

Tujuan penelitian ini untuk mendapatkan kapang fibrinolitik yang unggul daalm menghasilkan enzim fibrinolitik.  Isolasi kapang dilakukan dengan metode spread plate pada Potato Dextrose Agar.  Seleksi kemampuan proteolitik dan fibrinolitik dilakukan pada Skim Milk Agar dan fibrin plate agar.  Seleksi isolat berdasarkan indeks aktivitas enzim yang diperoleh dengan membagi diameter zona jernih di sekeliling koloni dengan diameter koloni.  Hasil seleksi memperoleh 41 isolat kapang fibrinolitik.  Lima isolat kapang dari 41 isolat kapang fibrinolitik mempunyai indeks aktivitas enzim 10.  Isolat tersebut teridentifikasi sebagai anggota dari Aspergillus (isolat S3, S4 dan R5) dan Penicillium (isolat G1 dan G2).
POTENSI INFUSA DAUN NANGKA SEBAGAI OBAT KUMUR HERBAL Christ Alfianus Tosubu; Nunung Sulistyani; Nur Khikmah
Jurnal Penelitian Saintek Vol 26, No 1 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i1.38032

Abstract

Penelitian ini dilakukan untuk mengkaji potensi daun nangka (Artocarpus heterophyllus) sebagai obat kumur herbal dengan melihat jumlah pertumbuhan koloni bakteri rongga mulut sebelum dan sesudah berkumur dengan infusa daun nangka. Daun nangka berwarna hijau muda yang diperoleh dari Babadan, Banguntapan, Bantul dibuat simplisia. Penentuan potensi dilakukan dengan menentukan perbedaan jumlah pertumbuhan koloni bakteri rongga mulut sebelum dan sesudah berkumur dengan infusa daun nangka. Uji potensi infusa daun nangka sebagai obat kumur dilakukan dengan menghitung perbedaan jumlah koloni bakteri rongga yang diperoleh dengan melakukan swab pada pangkal lidah sebelum dan sesudah berkumur. Perhitungan koloni bakteri dilakukan menggunakan metode hitung cawan (total plate count) pada media plate count agar (PCA). Hasil penelitian menunjukkan rata-rata jumlah koloni bakteri sebelum dan sesudah berkumur dengan infusa daun nangka yaitu 1,78 x 107 CFU/mL dan 7,71 x 106 CFU/mL. Perlakuan pemberian obat kumur infusa daun nangka secara signifikan mampu menurunkan jumlah koloni bakteri rongga mulut. Infusa daun nangka berpotensi sebagai alternatif obat kumur herbal. THE POTENCY OF JACKFRUIT LEAF INFUSION AS A HERBAL MOUTHWASHThis study was conducted to examine the potential of jackfruit (Artocarpus heterophyllus) leaves as a herbal mouthwash by identifying the growth of bacterial colonies in the oral cavity before and after gargling with jackfruit leaf infusion. The light green jackfruit leaves obtained from Babadan, Banguntapan, Bantul were made simplicia. The determination of potency was done by determining the difference in the number of bacterial colony growth in the oral cavity before and after gargling with jackfruit leaf infusion. The potential test of jackfruit leaf infusion as a mouthwash was carried out by calculating the difference in the number of cavity bacterial colonies obtained by swab at the base of the tongue before and after gargling. Bacterial colonies were counted using the total plate count method on plate count agar (PCA) media. The results showed that the average number of bacterial colonies before and after rinsing with jackfruit leaf infusion was 1.78 x 107 CFU/mL and 7.71 x 106 CFU/mL. The treatment of giving jackfruit leaf infusion mouthwash was significantly able to reduce the number of bacterial colonies in the oral cavity. Jackfruit leaf infusion has the potential as an alternative to herbal mouthwash.
ANTIBACTERIAL POTENTIAL OF JAMU INGGU AGAINTS SOME BACTERIA CAUSING UPPER RESPIRATORY TRACT INFECTION Nunung Sulistyani; Ardina Nugrahani
Jurnal Penelitian Saintek Vol 20, No 2: Oktober 2015
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1566.099 KB) | DOI: 10.21831/jps.v20i2.9595

Abstract

This study aims to examine the potential of jamu inggu as an alternative antibacterial herb that can be used against Staphylococcus aureus, Streptococcus pnuemoniae, S. Pyogenes, and Pseudomonas aeruginosa that is causing Upper Respiratory Infections (URI). Antibacteria activity test of jamu inggu was using the Agar diffusion method by Kirby-Bauer. To test jamu inggu antibacterial activity against Staphylococcus aureus, Streptococcus pnuemoniae, S. Pyogenes, and Pseudomonas aeruginosa, the diameter of the test bacteria growth inhibition zone was measured. The formation of a clear zone around the disc indicates that jamu inggu has chemical compounds that are antibacterial against Staphylococcus aureus,while the Pseudomonas aerugenosa, Streptococcus pyogenes, and S. pneumoniae bacteria cannot be inhibited by jamu inggu. It was indicated by no formation of a clear zone around the disc. Result shows that jamu inggu can be used as an alternative source of antibiotics against the Staphylococcus aureus.
THE RELATIONSHIP AMONG PEDICULOSIS CAPITIS, ANEMIA AND LEARNING ACHIEVEMENT IN ELEMENTARY STUDENTS Nunung Sulistyani; Nur Khikmah
Jurnal Penelitian Saintek Vol 24, No 2: Oktober 2019
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (24.683 KB) | DOI: 10.21831/jps.v24i2.26500

Abstract

This study was aimed at determining the relationship between the Pediculus humanus capitis infestation condition, anemia status and learning achievement in primary school-age children. This study was conducted at SD Bangunharjo Sewon Bantul, Yogyakarta. This study is an observational analytic study with cross sectional design in which the variables are measured in one measurement without intervening. The data obtained through pediculosis examination, Hb examination, and measurement of learning outcomes. The data then were analyzed descriptively presented in tabular form, then performed statistical analysis using the chi-square test. The results show that the students suffering from pediculosis, 57.9% also suffering from anemia and 51.0% having poor category achievement. In line with other researchers, one of the factors that influence one’s learning achievement is the memory factor. Someone’s anemia status can affect one’s memory. The prevalence of Pediculus humanus capitis infestation is 38.3%. Chi-square test showed a significant relationship between Pediculus humanus capitis infestation condition, anemia status, and learning achievement.HUBUNGAN PEDIKULOSIS KAPITIS, STATUS ANEMIA DAN PRESTASI BELAJAR PADA SISWA SEKOLAH DASAR Penelitian ini bertujuan untuk mengetahui hubungan kejadian infestasi Pediculus humanus capitis (kutu kepala) dengan status anemia dan prestasi belajar pada anak usia sekolah dasar. Penelitian ini dilakukan di SD wilayah Bangunharjo Sewon Bantul Yogyakarta. Penelitian ini merupakan penelitian analitik observasional dengan desain cross sectional. Variabel diukur dalam satu kali pengukuran dengan tidak melakukan intervensi. Data penelitian diperoleh melalui pemeriksaan pedikulosis, pemeriksaan Hb, dan pengukuran hasil belajar. Data dianalisa secara deskriptif yang disajikan dalam bentuk tabel, kemudian dilakukan analisis statistik menggunakan Uji chi-square. Hasil penelitian ini menunjukan bahwa menderita pedikulosis sebesar 57,9% menderita anemia dan sebesar 51,0% dengan prestasi belajar kategori kurang baik. Salah satu faktor yang mempengaruhi prestasi belajar seseorang yaitu faktor daya ingat. Status anemia seseorang dapat mempengaruhi daya ingat seseorang. Prevalensi infestasi Pediculus humanus capitis sebesar 38,3%. Uji chi-square menunjukkan adanya hubungan yang signifikan antara kejadian infestasi Pediculus humanus capitis dengan status anemia dan prestasi belajar.
Uji antibakteri teh hitam dan teh hijau kombucha pada methicillin-resistant Staphylococcus aureus (MRSA) Cyrilla Angelica Andhika Pramesti; Nur Khikmah; Nunung Sulistyani
Jurnal Biologi Udayana Vol 26 No 1 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i01.p12

Abstract

Teh kombucha memiliki aktivitas antibakteri pada bakteri Gram negatif, Gram positif, dan bakteri yang resisten terhadap antibiotik. Methicillin-resistant Staphylococcus aureus (MRSA) merupakan salah satu strain bakteri Staphylococcus aureus yang sudah mengalami resistensi terhadap antibiotika. MRSA merupakan penyebab utama infeksi nosokomial dan berbagai sindrom klinik. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri teh kombucha dan mengetahui perbedaan daya antibakteri teh hitam kombucha dan teh hijau kombucha dalam menghambat MRSA. Kombucha teh hitam dan kombucha teh hijau dibuat dengan fermentasi cair. Uji antibakteri teh kombucha pada MRSA dilakukan dengan metode difusi sumuran. Perbedaan antibakteri teh hitam kombucha dan teh hijau kombucha dianalisis menggunakan uji-t. Teh hijau kombucha memiliki kadar asam total yang lebih tinggi dengan pH yang lebih rendah dibandingkan teh hitam kombucha. Teh hitam kombucha dan teh hijau kombucha mempunyai aktivitas antibakteri pada MRSA yang ditunjukkan dengan terbentuknya zona irradikal dengan diameter 14 ± 0,85 mm dan 17,1 ± 0,93 mm. Zona irradikal menunjukkan aktivitas teh hitam kombucha dan teh hijau kombucha dikategorikan lemah. Oleh karena itu, teh kombucha belum efektif sebagai antibakteri terhadap MRSA. Hasil uji-t menunjukkan terdapat perbedaan aktivitas antibakteri antara teh hitam kombucha dan teh hijau kombucha pada Methicillin-resistant Staphylococcus aureus (MRSA).