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Induction of Barangan Banana shoot (Musa acuminata L.) from North Nias through Tissue Culture by giving 2,4-D and Kinetin Destarius Zebua; Suci Rahayu; Saleha Hanum
JBIO: jurnal biosains (the journal of biosciences) Vol 1, No 2 (2015): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v1i2.2780

Abstract

The research on induction of banana barangan shoot (Musa acuminata L.) from North Nias through tissue culture by giving 2,4-D and kinetin was conducted in the Laboratory of Tissue Culture  University of North Sumatera from May until October 2014. The main objective of this research was to know the explants of banana weevil in apical and basal position with growth regulator 2,4-D with kinetin which are able to induce shoots from callus initials. On this research, the treatment tested for induction of shoots was growth regulators 2,4-D in the concentration 0 mg/L, 1 mg/L, 1,5 mg/L, 2 mg/L and 2,5 mg/L with growth regulators kinetin in the concentration 0 mg/L, 5 mg/L, 6 mg/L, 7 mg/L and 8 mg/L. The research was designed using Completely Randomized Design (CRD) two factorial with repetition of experiment. The results of research showed that the interaction between concentration of 2,5 mg/L 2,4-D with 5 mg/L kinetin was fastest (79 days) forming the shoot derived from explant basal part. The concentration of 2,5 mg/L 2,4-D with 5 mg/L kinetin produced the average number of shoots formed for 3.00 and average number of highest shoots for 1.50 cm. Keywords: Musa acuminata, weevil, initiation, callus, shoot, induction, barangan banana, 2,4-D, kinetin.
Barangan Banana (Musa acuminata L.) Shoots Induction By Application Of NAA And BAP Based On The Sources Of Explant Basal Hardi Yudha; Suci Rahayu; Saleha Hanum
JBIO: jurnal biosains (the journal of biosciences) Vol 1, No 2 (2015): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v1i2.2782

Abstract

The research or barangan banana (Musa acuminata L.) shoot induction by application of NAA and BAP based on the source of explans basal that had been carried out in the system Culture Laboratory of the University of North Sumatra. This study aims to discover the source of explants forming shoots by application of NAA and BAP and the number of shoots formed by the application of NAA and BAP based sources of banana explants. The study uses a completely randomized design with two factorial, i.e. the concentration factor NAA (0 mg/l, 1.5 mg/l, 2.0 mg/l, 2.5 mg/l, and 3.0 mg/l) and concentration factor BAP (0 mg/l, 4 mg/l, 5 mg/l, 6 mg/l, and 7 mg/l) with the source explants apical and basal parts. The best position that created the shoots explants contained in explant basal part by the number of shoot formed is 83. In the treatment of 1.5 mg/l NAA and 5 mg/l BAP average number of shoots most 3,75 and 2,30 cm tall shoots, the formation of the shoots is in the 80th day. The application of NAA and BAP on basal explants have a significant influence on the formation of the shoots.   Key words: Musa acuminata L., NAA, BAP,explants basal, shoot.