Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : BERITA BIOLOGI

INFEKSI Salmonella enteritidis PADA TELUR AYAM DAN MANUSIA SERTA RESISTENSINYA TERHADAP ANTIMIKROBA Kusumaningsih, Anni; Sudarwanto, M
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.044 KB) | DOI: 10.14203/beritabiologi.v10i6.1944

Abstract

Salmonella enteritidis is one of pathogenic bacteria in chicken and human with re-emerging foodbome pathogen. Uncontrol use of antimicrobial drugs for the prevention and treatment of infectious diseases in chicken and human may lead to the development of antimicrobial resistance. Sampels of chicken eggs were collected from markets, layer farms, and grant parent stock farms. Sampels from human were anal swabs collected from hospitals and a laboratory of microbiology. Identification of bacteria was conducted by isolation and biotyping using selected media for Salmonella, while serotyping S. enteritidis was conducted with spesific 0 somatic (1, 9, and 12) and H flageIJa (m) antigen.Antimicrobial resistance tests were conducted by the standard diffusion method using antimicrobial disks.The results of isolation and identification of Salmonella show that 9 out of 122 (7.4%) sampels of layer eggs and 7 out of 23 (30.4%) sampels of embrioneted eggs were positive with Salmonella spp. The results of serotyping against S. enteritidis show that 7 out of 9 (77.0%) from layer eggs and 7 out of 7 (100.0%) from embrioneted eggs were positive with S. enteritidis.From the IS anal swabs of human were obtained 14 (93,3%) contain S. enteritidis.The antimicrobial resistance profiles of S. enteritidis isolated from layer eggs show high resistances to streptomycin (42.9%), neomycin (85.7%), doxicycline (64.3%), and ciprofloxacin (57,1%), whereas those S. enteritidis isolated from human show high resistances to streptomycin (50.0%), neomicyn (85.7%), tetracycline (42.9%), and doxicycline (42.9%).Multiple resistance profiles of S. enteritidis isolated from eggs were mostly to 2-3 antibiotics and those of S. enteritidis isolated from human were mostly to more than 5 antibiotics.
CEMARAN BAKTERI PATOGENIK PADA SUSU SAPI SEGAR DAN RESISTENSINYA TERHADAP ANTIBIOTIKA Kusumaningsih, Anni; Ariyanti, Tati
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (89.747 KB) | DOI: 10.14203/beritabiologi.v12i1.513

Abstract

Fresh milk is a beverage with high protein contents that can be consumed either directly or as ingredient supplement into safely and healthy food. However, the milk also as a good media for development of pathogenic bacteria that also dangerous for human health. The aim of this research was to determine pathogenic bacteria contamination in fresh milk and its antibiotic resistance profiles to several antibiotics. Fresh milk samples were taken from milk cans belong to the farmers at 34 dairy cows centre in Cibungbulang, Bogor, West Java. The quantitative determination was conducted on 34 milk samples. Several parameters examined were based on the Indonesian Nasional Standard (Standar Nasional Indonesia/SNI) No. 01-3141-1998 for Fresh Dairy Milk and SNI No. 7833-2009, such as total bacteria and coliform. The qualitative examination result for isolation and identification of bacteria were found that the milk samples consisted of 41.18% E. coli, 23.53% Streptococcus Gorup B, 8.82% Staphylococcus aureus, and none for Salmonella. The antibiotic resistence profiles were tested to 5 antibiotics. It showed that Escherichia coli isolates were resitance to penicilline (14.3%), oxytetracycline (21.4%), chloramphenicole (57.1%), and streptomycin (28.6%), whereas those Streptococcus Group B isolates were resistance to penicilline (12.5%), Oxytetracycline (37.5%), chloramphenicole (25.0%), streptomucin (87.5%), and ciprofloxacin (87.5%). Multiresistance of E. coli were found against 2 antibiotics, whereas Streptococcus against 2-3 antibiotics. This research indicated that fresh milk samples taken from farmers at Cibungbungang, Bogor were contaminated with several pathogenic bacteria and mostly highly resistance to 5 antibiotics testing.