The manufacture of industrial enzymes from trash provides a sustainable remedy for environmental issues. This work investigates machine learning methods to enhance enzyme production from industrial waste by examining critical factors such as waste type and chemical makeup. Three algorithms—Linear Regression, Decision Tree, and Neural Network—were used to estimate and forecast enzyme production. Evaluation criteria, such as Mean Squared Error (MSE) and Coefficient of Determination (R²), were used to evaluate model performance. The results indicated that the Decision Tree method was the most effective, exhibiting lowest error and enhanced accuracy in selecting ideal production factors such as fermentation temperature and time. This method improves efficiency, lowers operating expenses, and encourages sustainable waste management practices. The results highlight the potential of machine learning to convert trash into useful industrial goods, providing a route to more sustainable biotechnology. Future study may enhance hybrid algorithms, include new waste factors, and facilitate real-time implementation for wider industrial applicability.