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All Journal Majalah Obat Tradisional
Kurniati Pamungkas
Faculty of Pharmacy, Universitas Gadjah Mada
Health Professions Medicine & Pharmacology Public Health

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ISOLASI DAN PENETAPAN KADAR ALKALOID EKSTRAK ETANOLIK BUNGA KEMBANG SEPATU (Hibiscus rosa-sinensis L.) SECARA SPEKTRODENSITOMETRI Mimiek Murrukmihadi; Kurniati Pamungkas
Majalah Obat Tradisional Vol 20, No 2 (2015)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (595.842 KB) | DOI: 10.22146/tradmedj.8081

Abstract

This research aims to isolate and determine relative concentration of alkaloid in Hibiscus rosa-sinensis L flowers. using spectrodensitometry. Ethanolic extract of H. rosa-sinensis flowers was made by maceration methode using etanol 70% as solvent. Fractionation of alkaloid compound was using VLC (Vaccum Liquid Chromatography) and using organic solfent mixture of methanol-ethyl acetate in variation concentration. Isolate was used as standard curve, then the concentration of H. rosa-sinensis flowers extract was determined using spectrodensitometry with silica gel as stationer phase and ethyl acetate: methanol ( 1:5 v / v) as mobile phase. The relative concentration of alkaloid was calculated by using standard curve Y = 29023.75 X + 5809.35, r = 0.996. Determination of isolate showed the relative concentration of alkaloid was 0.210 ± 0.035 % and this can be used as marker compound.
Co-Authors Mimiek Murrukmihadi