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All Journal Majalah Obat Tradisional Jurnal Biodjati
Subagus Wahyuono
Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Medicine & Pharmacology Public Health

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AKTIVITAS ANTIOKSIDAN DAUN KESEMEK (Diospyros kaki L.F) DENGAN METODE DPPH (2,2-DIFENIL-1 PIKRILHIDRAZIN) Isnindar Isnindar; Erna Prawita Setyowati; Subagus Wahyuono
Majalah Obat Tradisional Vol 16, No 2 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.525 KB) | DOI: 10.22146/tradmedj.8338

Abstract

There are two groups of antioxidant resources, these are synthetic and natural antioxidants. The most important natural antioxidant is phenolic compounds that are produced naturally from plants. Phenolic Antioxidants are potent antiradical, biologically active as they are able to donate hydrogens to free radicals and break the chain of lipid oxidation reactions in the early stages. This study is aimed to search natural antioxidant compounds from the leaves persimmon (Diospyros kaki L.F) that have been utilized traditionally to prevent from sickness. In order to detect the presence of antioxidant compounds in the leaves extract, a reagent of DPPH (2,2-diphenyl-1-pykrilhidrazine) in methanol was used. Initially, powder of persimmon leaves (115 g) is macerated 3 times with methanol, 24 hours each time at room temperature. The liquid extract obtained is evaporated by rotavapor till residue (methanol extract) (phase I) is obtained. The residue was then extracted with CHCl3 to give CHCl3 soluble (phase II) and insoluble fractions. The later is then extracted with water to give water soluble (phase III) and insoluble fractions. These fractions (phase I-III) are chromatophed by TLC and sprayed by DPPH. Phase II contains antiradical compounds as shown by yellow color on the violet background. By preparative TLC [SiO2, CHCl3-EtOAc (1-4 v/v)], antiradical substance is isolated and identified tentatively as substituted phenolic compounds. Antiradical potential is measured spectrophotometrically, the isolat shows IC50 at 107.7 μg/mL that is lower than that of vitamin C (3.04 μg/mL).
Antioxidant Activity and Pancreatic Lipase Inhibition of Curcuma aeruginosa Roxb Rhizome Fractions Ririn Suharsanti; Subagus Wahyuono; Nunung Yuniarti; Puji Astuti
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33900

Abstract

Antiobesity with a lipase inhibitor mechanism will block the hydrolysis of triglycerides into fatty acids and glycerol, while antioxidant compounds are very useful in conditions of obesity to prevent excess damage from degenerative diseases. Curcuma aeruginosa Roxb extract has been proven to have the effect of inhibiting pancreatic lipase so further effects will be seen at the fraction level. The purpose of this research is to investigate the phytochemical components ,antioxidant activity and pancreatic lipase inhibition of Curcuma aeruginosa Roxb fractions. Ethanolic extract of the Curcuma aeruginosa Roxb rhizome was separated using the solid-liquid chromatography with 3 different solvents (n-hexane, ethyl acetate, ethanol) to give n-hexane (HF), ethyl acetate (EAF), and ethanol (EF), and the insoluble (IF) fractions. Each fraction detected phenolics, flavonoids, alkaloids, tannins, saponins, triterpenoids/steroids. EF has the highest total flavonoid and phenolic content. Antioxidant activity of all fractions were measured using DPPH reduction, ABTS, and FRAP methods. The best antioxidant activity of all fractions using the DPPH method was EF with IC50 21.93 ± 3.39µg/mL, ABTS method was HF with IC50 24.56±1,03 µg/mL and FRAP method was IF with IC50 20.79±1,03 µg/mL. Totals of phenolics and flavonoids in EF strongly support the antioxidant activity of the DPPH method. The highest inhibition of pancreatic lipase was found in EAF at 35.16±0.24 % (100 µg/ml). There was significant difference between EAF and xenical (orlistat) (p
Co-Authors Erna Prawita Setyowati Isnindar Isnindar Nunung Yuniarti Puji Astuti Ririn Suharsanti