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All Journal Jurnal Akuakultur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Aquacultura Indonesiana Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan

Asda Laining

Balai Penelitian dan Pengembangan Budidaya Air Payau, Maros

Author-ID : 304152

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Engineering Immunology & microbiology Veterinary

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Articles

Fatty Acid Profile of Hepatopancreas and Oocyte of Tiger Shrimp, Penaeus monodon, Fed Modified-commercial Diet by Supplementing Vitamin C and E Usman, Usman; Kamaruddin, Kamaruddin; Laining, Asda; Trismawanti, Ike
Aquacultura Indonesiana Vol 20, No 1 (2019)
Publisher : Indonesian Aquaculture Society (MAI)

Vitamin C and E have important roles in the process of reproduction, immune system, and anti-oxidants to protect cell membrane lipid, and the circulation of essential fatty acid in the body of the aquatic organism including shrimp. The aims of this study was to analyze the effect of vitamin C and E supplementation in modified-commercial diet on the fatty acids profile of hepatopancreas and oocyte of tiger shrimp, Penaeus monodon. The experimental treatments were: modified-commercial diet by supplementing with vitamin C (500 mg/kg) and E (300 mg/kg) (PS), and modified-commercial diet without vitamins supplementation (PK). Cultured tiger shrimp were used as the animal with initial weight of 43.1 ± 5.1 g (mean±STD) for females and 41.9 ± 4.4 g for males. The shrimps were cultured in two of 1000 m2 concrete ponds with a density of 100 shrimp / pond. During three months culture in the pond, they were fed the test at rate of 3% biomass/day. When the tiger shrimp reached the weight over 90 g for females and > 70 g for males, then the shrimp were transferred to concrete tanks for oocyte maturation evaluation. In maturation tank, the shrimp fed test diets (pellet) combined with fresh food (squid) as much as 3% (dry basis) at combination ratio of 50% : 50%. Shrimp reaching oocyte development stage III were dissected and sampled for hepatopancreas and oocytes for analysis of fatty acid profile. The results showed that the hepatopancrea and oocyte fatty acids contents were higher in the tiger shrimp fed PS compared to the tiger shrimp fed PK. The poly unsaturated fatty acid (PUFA) especially for arachidonic, docosahexaenoic, and eicosapentaenoic acid were higher in shrimp fed with PS than the shrimp fed PK. Despite higher total fatty acid contents in hepatopancreas than in oocyte for the both treatments, the esensial fatty acid arachidonic, docosahexaenoic, and eicosapentaenoic acid were higher in oocyte than in hepatopancreas of the shrimp. This suggested that these three types of PUFA played important role for oocyte development for female reproduction. Supplementation of vitamin C and E in the test diet had an effect on increasing PUFA content in hepatopancreas and oocytes of tiger shrimp.

Reproductive performances of wild male tiger shrimp Penaeus monodon post-injection of oocyte developer without eyestalk ablation Prasetyo, Dony; Laining, Asda; Sudrajat, Agus Oman
Jurnal Akuakultur Indonesia Vol. 16 No. 2 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The aim of this study was to evaluate the effect of oodev on the gonadal maturation and characteristic of spermatophore and spermatozoa produced by the wild male black tiger shrimp against eyestalk ablation. The treatments were two doses of oodev injection at 0.5 (OD0.5) and 1 (OD1.0) mL/kg of body weight and a control was eyestalk ablation (AB). The male stock of tiger shrimp used was from wild with body weight ranged from 55‒85 g, stocked into three of 10 tonnages concrete tanks with density of 25 males/tank. Oodev injection was applied for two times with one week interval. Tiger shrimp of OD0.5 group produced the highest number of gonadal maturing which was 84% followed by AB (68%) and OD1.0 (64%). Oodev injection was able to shorten the time required for spermatophore maturation at maturation phase which happened simultaneously within 7 days post injection compared to that of ablated males. Positive correlation (R2=0.612) was detected between the shrimp weight and weight of spermatophore of the tiger shrimp while correlation between spermatophore weight and number of spermatozoa was relatively low (R2=0.415). Total fatty acid tented to be higher in males injected with oodev compared to ablated males. Concentration of arachidonic acid in the muscle of male stock in OD1.0 group was extremely low of 0.0037% of total lipid compared to AB (0.3190%) and OD0.5 (0.2806%). Oodev injection at the dose of 0.5 mL/kg of tiger shrimp could improve the number of males stock producing spermatophore compared to eyestalk ablation. Simultaneously matured-spermatophore of wild male tiger shrimp within short time could be achieved through oodev injection. Keywords: oodev, spermatophore, reproduction, tiger shrimp ABSTRAK Tujuan penelitian ini adalah mengevaluasi efek hormon oodev terhadap pematangan gonad dan karakter spermatofor dan spermatozoa yang dihasilkan oleh induk udang windu jantan alam dibandingkan dengan teknik ablasi tangkai mata. Perlakuan yang dicobakan adalah dua dosis injeksi oodev yaitu 0,5 (OD0,5) dan 1 (OD1.0) mL/kg bobot tubuh dan kontrol yaitu ablasi tangkai mata. Induk udang windu jantan yang digunakan berasal dari alam berbobot antara 55‒85 g, ditebar dalam tiga bak pematangan gonad berkapasitas 10 ton dengan kepadatan 25 ekor/bak. Injeksi oodev dilakukan dua kali dengan interval satu minggu. Induk udang windu pada OD0,5 menghasilkan jumlah induk matang gonad tertinggi yaitu 84%, diikuti oleh AB (68%) dan OD1 (64%). Injeksi oodev mampu mempersingkat masa pematangan spermatofor pada fase maturasi dan pematangan tersebut terjadi secara simultan setelah 7 hari pascainjeksi dibandingkan dengan induk yang di ablasi. Korelasi positif (R2=0,612) dideteksi antara bobot tubuh dan bobot spermatofor induk udang windu, sedangkan korelasi antara bobot spermatofor dan jumlah spermatozoa relatif lemah (R2=0,415). Total asam lemak daging cenderung lebih besar pada induk udang windu jantan yang diinjeksi oodev dibandingkan yang diablasi. Konsentrasi arachidonic acid dalam daging induk yang diinjeksi OD1 sangat rendah sebesar 0,0037% dari lemak dibandingkan AB (0,3190%) dan OD0,5 (0,2806%). Injeksi oodev pada dosis 0,5 mL/kg udang windu dapat meningkatkan jumlah induk yang menghasilkan spermatofor dibandingkan ablasi tangkai mata. Pematangan gonad udang windu jantan alam secara simultan dan singkat dapat dilakukan melalui injeksi oodev. Kata kunci: oodev, spermatofor, reproduksi, udang windu

INFLUENCE OF TAURINE ON LARVAL EYE DEVELOPMENT AND GROWTH PERFORMANCE OF GOLDEN RABBITFISH (Siganus guttatus) Darsiani, Darsiani; Setiawati, Mia; Jusadi, Dedi; Suprayudi, Muhammad Agus; Laining, Asda
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.2.2057

ARTICLE HIGLIGHTS- Taurine improves eye development in golden rabbitfish larvae- Enhanced growth performance with taurine-enriched rotifers- Taurine positively impacts fish eye and body growthABSTRACTGolden rabbitfish (Siganus guttatus) is an emerging species for aquaculture industry, despite constrains faced on commercial scale of seed production due to the high mortality during the first-feeding stage. An experiment was conducted to determine the effect of taurine through enrichment of rotifers as live-feed on eye development and growth performance of larval golden rabbitfish. Observation of eye development was carried out by measuring eye diameter of the larva and development of the retina based on histology assessment. Growth performance was measured for absolute growth and fin development of the larvae and survival rate. The results showed that taurine-enriched rotifer generally provided better performances for eye development and growth of larval golden rabbitfish compared with control (without taurine). Increase of taurine dose up to 0.050 g/L resulted in an increase of eye diameter, absolute growth, fin development and survival rate. Further increased increment of the taurine tended to decrease the values of the measured parameters. The eye diameter of larval golden rabbitfish fed with 0.050 g/L taurine-enriched rotifer was significantly wider (106.1±9.8 µm) (P < 0.05) compared with control (58.2±14.3 µm), but did not significantly differ from other doses of taurine (P > 0.05). Body width of larvae fed with 0.050 g/L taurine-enriched rotifer was significantly higher (127.3±14.6 µm) (P < 0.05) compared with control (98.8±18.3 µm). In regard to eye development, growth performances (total length, fin development and survival rate), dose of taurine for rotifer enrichment fed to larval golden rabbitfish Siganus guttatus was 0.050 g/L.

Development of Siganid (Siganus guttatus) larvae during the transition period Darsiani, Darsiani; Setiawati, Mia; Jusadi, Dedi; Suprayudi, Muhammad Agus; Laining, Asda
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.1.22296

Siganid is better known as rabbit fish. In hatcheries, constraint that is still faced is the low survival, which was assumed to occur because of the timing for initial feeding is not solidly known. This research aimed to examine the best initial feeding time for siganus, based on evaluation on eyes and yolk reserves during the transition. The research was conducted from 24-29 March 2021 in IPUW Barru, South Sulawesi. Larvae were obtained from the second progeny (G2) of domesticated Siganus guttatus. Larvae were reared for 5-6 days without feeding. Evaluated parameters include eyes diameter and yolk reserves. Samples were observed with microscope and will be explained descriptively. Water quality parameters were measured, namely DO, salinity, pH and temperature. Eyes diameter at 6 Hour After Hatching (HAH) ranges between 81.5-128.9 m, 13 HAH= 125.5-167.7 m, 24 HAH= 138.2-213.9 m, two days after hatching 2 Day After Hatching (DAH) = 113.6-193.1 m, 3 DAH= 163.1-219.2 m, 4 DAH= 190.4-212.6 m. Yolk reserves diameter ranged between 137-194m (6 HAH), 13 HAH= 152-191m, 24 HAH= 94.0-185m, 2 DAH= 75.3-99.63m, 3 DAH= 42.33-87.58m, 4 DAH= 38.17-55.59m. After age 5 DAH, there are no larvae found alive (dead). Eyes developed at age 6 HAH and experienced pigmentation at age 24 HAH. Conversely, yolk reserves diameters were getting smaller since age 24 HAH and completely disappear at age 4 DAH. It indicates that eyes effectively see feeds at age 2 DAH. Therefore, initial feeding should be started. The water quality parameters measured were still in normal conditions according to the life of S. guttatus larvae. From this research, it can be concluded that eyes have been well functioned at age 2 DAH and yolk reserves was finished at age 4 DAH. Therefore, the initial feeding should be done at the age of 2 DAH.Keywords:Development, Siganus guttatus larvae,Transition

Co-Authors Agus Oman Sudrajat darsiani, Darsiani DEDI JUSADI Dony Prasetyo Kamaruddin Kamaruddin Mia Setiawati MUHAMMAD AGUS SUPRAYUDI Trismawanti, Ike Usman Usman

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