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Dewa Ayu Agus Sri Laksemi
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Education Health Professions Medicine & Pharmacology Nursing Public Health

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GAMBARAN PENGETAHUAN DAN KONTAMINASI SOIL TRANSMITTED HELMINTHS (STH) SAYUR KUBIS (Brassica oleracea L.) DAN KEMANGI (Ocimum sanctum L.) PADA PEDAGANG LALAPAN DI DESA X, DENPASAR SELATAN Putra, I Putu Bagus Krisna Pramana; Laksemi, Dewa Ayu Agus Sri; Diarthini, Ni Luh Putu Eka; Damayanti, Putu Ayu Asri
E-Jurnal Medika Udayana Vol 13 No 8 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i08.P11

Abstract

Soil Transmitted Helminths (STH) infection is caused by nematodes transmitted through the soil. In general, such as Ascaris lumbricoides, Trichuris trichiura, hookworm, or Strongyloides stercoralis. A habit of consuming not hygienic vegetables or lalapan like cabbage (Brassica oleracea L.) and basil (Ocimum sanctum L.) is a risk for transmission. Lalapan merchants play important role in food preparation, as of knowledge and good food safety are required. The study aimed to describe STH knowledge, personal hygiene, water sources, lalapan merchant’s safekeeping, and STH contamination in cabbage and basil. The study is descriptive cross-sectional with STH knowledge, STH determinants questionnaire, and microscopic identification with NaCl 0,9% sedimentation method for instruments. The sample was all lalapan merchants in X Village, South Denpasar. Total there are 26 respondents. 84,62% of respondents are male, 34,6% at age 17-25 years old, and 65,4% senior high school as tertiary education. Obtained, lalapan merchant’s knowledge is not good at 57,7%. All respondents with clean fingernails and 92,3% practiced hand washing. Water sources from municipal waterworks (76,9%), clean merchant’s water containers (88,5%), and closed (92,3%). The food safekeeping was clean, separated in 92,3%, and closed for 88.5% of respondents. Contamination was found in 8,6% of 58 samples. Hookworm eggs in three basil (10,3%) and one cabbage (3,4%), one larva STH in basil (3,4%). Although knowledge is not good, personal hygiene is widely practiced. Water sources are mostly from municipal waterworks. The food safekeeping is separated, closed, and clean. It’s necessary to improve the knowledge about STH infection to realize food safety principles. Keywords : soil transmitted helminths, STH knowledge, STH contamination
Prevalensi Infeksi Parasit Usus pada Siswa SD Negeri 5 Subagan Daerah Wisata Jasri Kabupaten Karangasem Tahun 2022 Suputra, I Wayan Bagus Abiyoga; Diarthini, Ni Luh Putu Eka; Laksemi, Dewa Ayu Agus Sri; Damayanti, Putu Ayu Asri
E-Jurnal Medika Udayana Vol 12 No 12 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i12.P15

Abstract

Infeksi parasit usus merupakan salah satu penyakit tropis terabaikan yang paling banyak terjadi pada anak-anak di seluruh dunia, termasuk di Indonesia. Kejadian infeksi parasit usus sangat dipengaruhi oleh sanitasi lingkungan dan masyarakat. Penelitian ini bertujuan untuk mengetahui prevalensi infeksi parasit usus pada anak-anak SD Negeri 5 Subagan, Desa Jasri, Kabupaten Karangasem pada tahun 2022. Penelitian ini berhasil mengumpulkan spesimen tinja dan kuesioner dari 121 anak. Spesimen tinja tersebut diperiksa secara mikroskopis dengan menggunakan metode direct wet mount. Hasil penelitian menunjukkan bahwa sebanyak 10 anak (8,3%) terdeteksi mengalami infeksi parasit usus. Kasus infeksi parasit usus pada penelitian ini terdiri dari kasus infeksi tunggal yang disebabkan oleh Giardia lamblia (4/121; 3,3%), Blastocystis spp. (3/121; 2,5%), Trichuris trichiura (1/121; 0,8%), Hymenolepis diminuta (1/121; 0,8%) serta kasus infeksi campuran yang disebabkan oleh Giardia lamblia dengan Taenia sp. (1/121; 0,8%). Penelitian ini menyimpulkan bahwa prevalensi infeksi parasit usus pada anak-anak SD Negeri 5 Subagan, Desa Jasri, Kabupaten Karangasem tahun 2022 termasuk dalam kategori rendah.
PREVALENSI INFEKSI CACING USUS PADA SISWA SEKOLAH DASAR DI DESA PED KABUPATEN KLUNGKUNG Dyatmika, I Nyoman Adidana Jaya; Diarthini, Ni Luh Putu Eka; Laksemi, Dewa Ayu Agus Sri; Damayanti, Putu Ayu Asri
E-Jurnal Medika Udayana Vol 12 No 8 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i08.P05

Abstract

Background : Intestinal worms is still a health problem in Indonesia, intestinal worm infections are included in neglected disease, which means this disease has not received special attention from the sufferer. Ped Village, Klungkung Regency is an area with warm temperatures, sandy loamy soil, and clean water has not been reached. This factors raise the suspicion that in Ped Village, Klungkung Regency there is one or more species of intestinal worms, so it is very important to determine the prevalence of intestinal worm infections in elementary school students Ped Village, Klungkung Regency. Aims : To determine prevalence of intestinal worm infection in elementary school students in Ped Village, Klungkung Regency. Methods : This study uses a descriptive cross sectional method, where researchers will determine the prevalence of intestinal worm infection through fecal samples examined by the kato-katz method, intestinal worms are identified under a microscope with an objective magnification of 10x to 40x. Results : There were 139 respondents who met the inclusion criteria of the study, including 72 men and 67 women, from the results of laboratory tests, prevalence of intestinal worm infection was 0.7%. Infection occurs in male children with the worm species Enterobius vermicularis. Conclusion : The prevalence of intestinal worm infection was 0.7% in elementary school students Ped Village, the species found was Enterobius vermicularis in boys. The cause of this infection is thought due to environmental and personal hygiene factors which are still not well maintained in students. Keywords: Intestinal worm infection, prevalence, elementary school children, Ped village
THE EFFECT OF PANDAN WANGI ROOTS ETHANOL EXTRACT (Pandanus amaryllifolius Roxb.) ON THE DEGREE OF PLASMODIUM BERGHEI PARASITEMIA IN VIVO Saraswati Devi, Ni Made Kumbha Mella; Laksemi, Dewa Ayu Agus Sri; Diarthini, Ni Luh Putu Eka; Sudarmaja, I Made
E-Jurnal Medika Udayana Vol 13 No 2 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i02.P13

Abstract

Malaria is an disease caused by Plasmodium genus and transmitted by female Anopheles mosquitoes via bites. The occurrence of antimalarial drug resistance in various places, such as Chloroquine and Artemisinin Combination Therapy, be a challenge for researchers to find new herbal and effective alternative antimalarial drugs. Pandan Wangi roots (Pandanus amaryllifolius Roxb.) contain compounds with antimalarial properties. This study aims to determine the effect of Pandan Wangi roots ethanol extract at doses of 1, 100, and 1,000 mg/kgBW (Body Weight on the degree of Plasmodium berghei parasitemia in vivo. This study employs an experimental in vivo method using the Post-Test Only Controlled Group Design. Sample of 24 mice were divided into one negative control group and three treatment groups. The data analysis technique employed is One Way ANOVA and Post Hoc Test. In this study, the mean degree of parasitemia in negative control group was 32.60%, treatment group with dose of 1 mg/kgBW was 22.27%, treatment group with dose of 100 mg/kgBW was 18.49%, and treatment group with dose of 1,000 mg/kgBW was 14.88%. The One Way ANOVA analysis resulted in p-value of 0.001, which is < 0.05, indicating a significant difference in the average degree of parasitemia among the three treatment groups. Thus, the effect of Pandan Wangi roots ethanol extract has antimalarial activity. Administration dose of 1,000 mg/kgBW proved to be the most effective in suppressing the growth of Plasmodium berghei parasitemia. Keywords : Pandanus amaryllifolius Roxb., Antimalarial, Plasmodium berghei, Degree of Parasitemia
GAMBARAN TINGKAT KECEMASAN MAHASISWA FAKULTAS KEDOKTERAN UNIVERSITAS UDAYANA SEMESTER PERTAMA DALAM MENGHADAPI UJIAN OBJECTIVE STRUCTURED CLINICAL EXAMINATION Harisutha, Made Dwiki Pradnyana; Diarthini, Ni Luh Putu Eka; Laksemi, Dewa Ayu Agus Sri; Wardani, Ni Putu
E-Jurnal Medika Udayana Vol 11 No 3 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2022.V11.i03.P05

Abstract

Anxiety experienced by medical students when compared to the general population of the same age has a greater prevalence and is at a higher level. Anxiety can be one of some factors that can affect exam performances which can be a determining factor in medical students’ academic achievement. One of the conditions that can cause medical students to experience anxiety in the educational process is the result of stress in facing the Objective Structured Clinical Examination (OSCE). The OSCE results can be affected by students’ exam performances. This study aims to describe the anxiety levels in first semester students of the Udayana University Faculty of Medicine in facing OSCE. This study applied cross-sectional descriptive method using primary data of anxiety levels on first semester medical students who will face OSCE for the first time dan secondary data of students’ OSCE results. Zung Self-Rating Anxiety Scale (ZSAS) questionnaire were used to collect the primary data which were retrieved only once for each student. During the study, 106 respondents data were collected, consisting of 17 to 21 years range of age, and most of them were females (61.32%). This study was resulting in the form of a description of the level of anxiety towards OSCE with the most occur anxiety level towards OSCE in medical students is mild anxiety level (84.91%), followed by moderate anxiety level (14,15%), and severe anxiety level (0,94%). The results can be used to present a basic data to conduct further research in Udayana University Faculty of Medicine as a contribution for improving student readiness in facing OSCE. Keywords: anxiety levels, objective structured clinical examination, medical students.
THE EFFECT OF JOHAR PLANT FLOWER EXTRACT “CASSIA SIAMEA” ON THE DEGREE OF PLASMODIUM BERGHEI PARASITEMIA IN VIVO Pandango, Ivana Griselarosa Koni; Laksemi, Dewa Ayu Agus Sri; Sudarmaja, I Made; Diarthini, Ni Luh Putu Eka
E-Jurnal Medika Udayana Vol 13 No 3 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i03.P09

Abstract

Malaria is a disease caused by a parasite of the genus Plasmodium sp. that spreads through the bite of the female Anopheles mosquito. Malaria is still a global problem because almost half of the world’s population is at risk of malaria which is endemic to 86 tropical and subtropical countries. In addition, resistance to antimalarial drugs that occur in some places makes many researchers try to find antimalarial drugs made from natural ingredients so that they can reduce the risk of resistance with minimal effects. One natural ingredient that is thought to be used as an antimalarial drug is the johar flower (Cassia siamea). The objective of this study is to determine the effect of johar flower extract (Cassia siamea) at doses of 10 mg/kgBW, 100 mg/kgBW, and 1000 mg/kgBW on the degree of parasitemia in male mice Balb/c strain infected with Plasmodium berghei. This study is an experimental study in vivo using the design of the Randomized Post-test Only Controlled Group method using 24 samples of mice which were divided into four groups, one negative control group and three treatment groups. The average degree of parasitemia was obtained in the control group of 24.9%, the 10 mg/kgBW dose treatment group of 13.4%, the 100 mg/kgBW dose treatment group of 10.3% and the 1000 mg/kgBW dose treatment group of 12.2%. Based on the results of the analysis with One-way ANOVA, an average difference was found between the control and treatment groups with a p-value <0.001. The result showed that giving johar flower extract (Cassia siamea) had an influence on antimalarial activity in reducing the percentage of parasitemia of mice infected with Plasmodium berghei by looking at the average difference between the control group and the treatment and obtained the average results of the treatment group of 100 mg/kgBW and 1000 mg/kgBW had the highest influence in reducing the degree of parasitemia. Keywords: Cassia siamea, Antimalaria, Plasmodium berghei, Degree of Parasitemia
UJI AKTIVITAS BUAH MAHKOTA DEWA (PHALERIA MACROCARPA) SEBAGAI ANTIMALARIA PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI Juniantara, I Kadek Ade; Laksemi, Dewa Ayu Agus Sri; Damayanti, Putu Ayu Asri; Diarthini, Ni Luh Putu Eka
E-Jurnal Medika Udayana Vol 11 No 12 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2022.V11.i12.P15

Abstract

Malaria is a parasitic infection caused by Plasmodium sp. Malaria’s medication and prevention are known, but there is a problem of increasing Plasmodium resistance to antimalarial drugs such as drugs from the chloroquine class, to overcome this problem traditional medicine is chosen because it is safer and cheaper. God’s crown (Phaleria macrocarpa) is one of the plants commonly used for traditional medicine which contains several active substances, namely saponins, polyphenols, flavonoids, tannins, sterols, alkaloids and terpenoids which have potential as antimalarials which can be proven by observing the degree of parasitemia in the mice blood. This study was conducted in vivo using the Randomized Post Test Only Controlled Group method using 24 samples of mice which were divided into 4 groups. The negative control group was only 0.2 ml of RPMI as a treatment control and the treatment group was treated with God’s crown extract at doses of 1, 10, and 100 mg/kgBW, then the degree of parasitemia was examined in each mouse. The data analysis technique used is One Way ANOVA and Post Hoc test. The results obtained mean the degree of parasitemia in the control group, doses of 1 mg/kgBW, 10 mg/kgBW and 100 mg/kgBW respectively 41.70; 37.96; 31.55; and 37.0. The results based on the analysis using One Way ANOVA posttest, it was found that there were significant differences between the control group and treatment groups 1,2, and 3 with p value of 0.000 (p < 0.05). The results showed that the extract of the fruit of the God’s crown (Phaleria macrocarpa) could inhibit the growth of Plasmodium berghei, the best dose was found at a dose of 10 mg/kgBW.
PENYULUHAN EFEKTIF MENINGKATKAN PENGETAHUAN PEMBERANTASAN JENTIK NYAMUK AEDES AEGYPTI PADA ANAK SEKOLAH DASAR DI KOTA GIANYAR Damayanti, Putu Ayu Asri; Laksemi, Dewa Ayu Agus Sri; Diarthini, Ni Luh Putu Eka
Coping: Community of Publishing in Nursing Vol 12 No 1 (2024): Februari 2024
Publisher : Program Studi Sarjana Ilmu Keperawatan dan Profesi Ners, Fakultas Kedokteran, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/coping.2024.v12.i01.p03

Abstract

Nyamuk Aedes aegypti merupakan vektor penyakit Dengue yang masih endemis di Bali termasuk di Kabupaten Gianyar. Pencegahan penyakit dapat dilakukan dengan melakukan pengendalian vektor, yaitu dengan melakukan pemantauan dan pemberantasan jentik nyamuk. Pemberantasan jentik nyamuk dilakukan secara mandiri oleh segenap masyarakat dimulai dari seluruh anggota keluarga termasuk anak usia sekolah dasar (SD). Sebagai langkah awal anak SD harus dibekali pengetahuan tentang jentik nyamuk agar bisa melakukan pengendalian jentik. Salah satu metode peningkatan pengetahuan yang efektif adalah dengan metode penyuluhan. Penelitian ini bertujuan untuk mengetahui efektifitas penyuluhan dengan metode ceramah yang didukung media audiovisual pada anak SD Negeri 1 Kota Gianyar dalam meningkatkan pengetahuan tentang pemberantasan jentik nyamuk. Rancangan penelitian adalah analitik cross-sectional dengan melibatkan 92 siswa SD kelas V. Pengukuran pengetahuan dengan kuesioner dilakukan sebelum (pre-test) dan sesudah penyuluhan (post-test). Hasil penelitian menunjukan median skor pengetahuan pre-test dan post-test sebesar 50 dan 80. Terdapat perbedaan signifikan pengetahuan sebelum dan sesudah penyuluhan (p=<0,01) dengan peningkatan pengetahuan sebesar 37,5%. Dapat disimpulkan bahwa penyuluhan efektif meningkatkan pengetahuan anak SDN 1 Kota Gianyar. Pendidikan kesehatan tentang pemberantasan jentik sejak dini dilakukan secara rutin dan simultan demi mendukung program pengendalian vektor penyakit Dengue.
Co-Authors Dyatmika, I Nyoman Adidana Jaya Harisutha, Made Dwiki Pradnyana I Made Sudarmaja Juniantara, I Kadek Ade Ni Luh Putu Eka Diarthini, Ni Luh Putu Eka Ni Putu Wardani Pandango, Ivana Griselarosa Koni Putra, I Putu Bagus Krisna Pramana Putu Ayu Asri Damayanti Saraswati Devi, Ni Made Kumbha Mella Suputra, I Wayan Bagus Abiyoga