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C. Lauridsen
Faculty of Science and Technology, Aarhus University, AU-Foulum, Tjele, 8830
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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INFLUENCE OF AGE ON THE SUSCEPTIBILITY OF PIGLETS TO ESCHERICHIA COLI O138:F18 Sugiharto, S.; Lauridsen, C.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 3 (2014): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.3.159-166

Abstract

The ex vivo and in vivo studies were undertaken to examine age-effect on the susceptibility of pigsto E. coli F18-diarrhea. The ex vivo experiment was conducted with 2 and 4 weeks old pigs based on theporcine intestinal organ culture (PIOC) model. The in vivo experiment was performed with 2 and 3weeks old pigs fed milk replacer and inoculated with E. coli F18. E. coli F18 inoculation to the intestinalcultures resulted in higher (P<0.01) counts of E. coli associated to the intestinal tissue, but suchdifference was not observed between the 2 and 4 weeks old pigs. Faecal dry matter (DM) decreased(P=0.01), whereas the proportion of pigs with diarrhea and faecal haemolytic E. coli counts increased(P<0.05) following inoculation, however, the difference between the ages was not observed in the invivo study. The interaction (P=0.01) between age and sampling day was observed for the number of totalwhite blood cells (WBC). The proportion of neutrophils decreased (P=0.01), whereas the lymphocytes,red blood cells (RBC), hemoglobin (Hb) and hematocrit (Hct) increased (P<0.01) after inoculation, butthe differences between age groups were not observed regarding these variables. In conclusion, age (2versus 3 or 4 weeks) did not influence the susceptibility of piglet to E. coli F18-diarrhea.
FATTY ACIDS PROFILE OF MEAT, MUCOSAL sIgA CONCENTRATION AND PRODUCTION INDEX OF BROILER AS A RESPONSE TO CHLORELLA sp. ADMINISTRATION IN THE DIET Sugiharto, S.; Henckel, P.; Lauridsen, C.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 3 (2010): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.3.172-178

Abstract

This study was carried out to investigate the effect of different levels of Chlorella sp. in the form ofmeal administrated in the diet of broilers throughout their life upon FA profile of the breast muscle,mucosal secretory IgA (sIgA) concentration and production index (PI) of broiler. Allotted in 18 pens, thetotal of 90 Ross day old chicks were assigned in completely randomized design by 3 of dietarytreatments (T1: control (basal diet without enrichment of Chlorella sp.); T2: basal diet enriched with 5-gChlorella sp./kg feed; T3): basal diet enriched with 10-g Chlorella sp./kg feed. At d-36, skinless breastmeat was collected for FA determination. Practical standard of vaccination was performed to activateantibodies production and at d-36 gut mucosa was collected for sIgA analysis. Chlorella sp.administration had no significant effect (P>0.05) on the FA profile of breast muscle, mucosal sIgAconcentration and producton index of broiler. In conclusion, the level and ratio between n-3 to n-6polyunsaturated fatty acids (PUFA) as well as the nature of dietary PUFA source determine tissue PUFAcomposition. Beside through eicosanoid, Chlorella sp. may affect mucosal sIgA production throughcytokines mediated effect. Although Chlorella sp. administration does not improve the production indexof broiler, this treatment may produce broiler meat with lower fat content.
Co-Authors P. Henckel S. Sugiharto