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S. Sugiharto
Department Of Animal Science, Faculty Of Animal And Agricultural Sciences, Diponegoro University
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The Gonad Maturity of Female Osteochillus vittatus in the presence of Ascorbic Acid Setyaningrum, Nuning; Sugiharto, Sugiharto; Hidayah, Hexa Apriliana
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9848

Abstract

The fishs reproduction status is affected by both fed compositions and vitamins intake lead to determine improvement of eggs quality as well as fish production. The presence of Ascorbic Acid (AA) in the cultivation ponds, might accelerate females gonad maturation and so rematuration. The research aimed to determine: (1) Level gonad maturity of supplementation Ascorbic Acid; (2) Oocyts diameter; (3) Larvae survival rate. The research used experimental methods.The method was a completely randomized design (CRD) of 4 treatments and 4 replications. Treatments were supplementation of AA at different dosages of 0, 600, 1200 and 1800 mg/kg fish-fed ration.The gonad maturity level was analysed descriptively and oocyte diameter and larvae survival rate data were analysed by ANOVA. The result showed that supplementation of AA at a dosage of 1200 mg / kg fish-fed ration accelerated the process of gonad maturity, development of oocyt diameter and larvae survival rate as well as the viability of O. vittatus larvae at 90 rearing days. In this case, level IV gonad maturity was reached at 90 days which characterized by completed vitelogenesis process and oocyte diameter of 1.1 mm. Thus, this study is useful for aquaculture science by providing information on utilization of AA as food supplement in fish culture and also for fish farmer who wish to accelerate O. vittatus reproduction.
Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis Wahyuningsih, Sri Puji Astuti; Pramudya, Manikya; Sugiharto, Sugiharto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4969

Abstract

Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains ?-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1) and adding polysaccharide krestin before-after exposure (P3) had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 0.523 M and 1.456 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2) had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 97.96 M and 953.86 328.16 M respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., & Sugiharto, S. (2016). Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology & Biology Education, 8(1), 12-17.
Effects of Some Feed Suplements Types to the Growth of Javaen Barb/Brek Fish (Puntius orphoides) Second Filial as Domestication Product Susatyo, Priyo; Sugiharto, Sugiharto; Hana, Hana; Chasanah, Titi
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5517

Abstract

Aquaculture can be improved by a diversified approach in the form of domestication research of a wild fish. The research has been done by cultivating Javaen Barb/Brek (Puntius orphoides) from Serayu river, Banyumas at a natural pond and laboratory scale. Brek fish is relative slow in growth and development of gonad. This research is aimed to determine: (1) the rate of general growth of Brek in the provision of some kinds of additional food; (2) Relative Growth Rate (RGR); (3) Specific Growth Rate (SGR) and Feed Conversion (FC). The research used experimental methods. The method was a completely randomized design (CRD) of 4 treatments and 3 replicationsof: A = 100% pellet; B = 70% soybean sprout and 30% pellet; C = 70% Ipomoea aquatica leaf and 30% pellet; D = 35% soybean sprouts, 35% I. aquatica leaf and 30% pellet. Quantitative data were analysed by analysis of variance (ANOVA) of the fishes growth.The results show influence of feed types to the Relative Growth Rate (RGR) of Javaean Barb. The best RGR with the value of 72.40 was given by feed types in the D combination. Meanwhile, Specific Growth Rate (SGR) and Feed Conversion (FC) were not affected by the feed types. The study were expected to provide suggestions for efficient feeding techniques in attempts at domestication of wild fish.How to CiteSusatyo, P., Sugiharto, S., Hana, H., & Chasanah, T. (2016). Effects of Some Feed Suplements Types to The Growth of Javaen Barb/Brek Fish (Puntius orphoides) Second Filial As Domestication Product. Biosaintifika: Journal of Biology & Biology Education, 8(3), 278-285.
JUMLAH LEUKOSIT DAN DIFFERENSIASI LEUKOSIT AYAM BROILER YANG DIBERI MINUM AIR REBUSAN KUNYIT (The Number of Leukocyte and Differentiation Leukocyte of Broiler Chickens that be Given the Boiled Turmeric Water on Drink) Pristiwanti, Pristiwanti,N.; Sugiharto, Sugiharto; Isroli, Isroli
Jurnal Pengembangan Penyuluhan Pertanian Vol 14, No 1 (2017): Jurnal Pengembangan Penyuluhan Peternakan
Publisher : UPPM Politekik Pembangunan Pertanian Yogyakarta Magelang (Polbangtan Yoma)

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh air kunyit rebus terhadap leukosit dan differensiasi leukosit ayam broiler. Penelitian dilakukan pada bulan Oktober - November 2016. Materi yang digunakan adalah 200 ekor ayam kampung (DOC) ayam pedaging dengan berat awal 41,48 ± 0,99 g. Pakan yang digunakan adalah pakan komersialdengan kode B-11S untuk stater fase dan BR-1AJ untuk fase finisher dan kunyit. Desain yang digunakan dalam penelitian ini adalah rancangan acak lengkap (RAL) dengan 5 perlakuan dan 5 ulangan yang masing masing terdiri dari 8 ekor DOC. Perlakuan terdiri dari T0 (100% air), T1 (75% air + 25% air rebusan kunyit), T2 (50% air + 50% air rebusan kunyit), T3 (25% air + 75% air rebusan kunyit) T4 (100% air rebusan kunyit). Hasil penelitian menunjukkan bahwa air kunyit rebus memberikan pengaruh yang signifikan terhadap persentase limfosit dan eosinofil (P <0,05), namun tidak berpengaruh secara signifikan terhadap jumlah total leukosit, persentase heterofil dan monosit (P> 0,05).Kesimpulan dari penelitian ini adalah bahwa air kunyit rebus dalam air minum ayam pedaging mampu memperbaiki profil darah putih, yang ditunjukkan dengan jumlah leukosit dan persentase limfosit dan eosinofil.Kata kunci: Kunyit, leuycocyte, leucocyte diferensial, ayam pedaging
Population of Esteochilus spp. as a Base of Sustainable Fishing in Serayu River Lestari, Windiarini; Putranto, Yogi; Setyaningrum, Nuning; Sugiharto, Sugiharto
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.9

Abstract

The increasing demand for freshwater resources generated by human population growth, urbanization, industrialization and irrigation will result in a decline or loss of freshwater fish such as Osteochilus spp. The aims of research were to analysis the population of Osteochilus spp in terms of abundance, longitudinal distribution and sex ratio and to determine environmental factors of river driven these populations. The research was carried out in upper part of Serayu River during wet season (January – March) and dry season (June - August) 2007. Fish was collected by using electro fishing and stratified random sampling was applied by dividing this river into five strata based on altitude. The result showed that the total abundances of Osteochilus spp. was 149 individuals, it was composed by of O. hasselti (47) with 8.30 – 24.35 cm and 11.20 – 198.70 g, O. microchephalus (101) with 11.35 – 25.21 cm and 17.9 – 206.5 g and O. kahajanensis (1) with 17.30 cm and 68.80 g. The size of population O hasselti and O. microchephalus gradually increased from 7 and 4 individuals in stratum A became 27 and 12 individuals in stratum D, and then increased up to 43 and 17 individuals in stratum E. The sex ratio of O. hasselti was 10:16 and O. microchephalus was 20:23. Both species were longitudinally distributed in Serayu River. It seems that the population of O. hasellti and O. microchephalus were more influenced by physical factors i.e. depth and current rather than chemical factors i.e. DO and pH of Serayu River.
PENGARUH PENAMBAHAN PROBIOTIK Rhizopus oryzae TERHADAP TOTAL MIKROBA USUS HALUS DAN SEKA AYAM KAMPUNG PERIODE GROWER (The Effect of Probiotic Rhizopus Oryzae Administrationon The Total Number of Microbes in The Small Intestine and Caeca of Kampoeng Chicke Pratiwi, Kristiani Dina; Sugiharto, Sugiharto; Yudiarti, Turrini
Animal Agriculture Journal Vol 3, No 3 (2014): Volume 3, Nomor 3, Tahun 2014
Publisher : Animal Agriculture Journal

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Abstract

ABSTRAK Penelitian bertujuan mengetahui pengaruh penambahan probiotik Rhizopus oryzae ayam kampung terhadap jumlah total mikroba yang ada pada usus halus dan seka ayam kampung periode grower. Penelitian dilaksanakan pada tanggal 11 Agustus–11 Oktober 2014 di kandang ayam, Fakultas Peternakan dan Pertanian, Universitas Diponegoro.Materi yang digunakan adalah Day Old Chick (DOC) ayam kampung sebanyak 100 ekor (unsexed) dengan bobot badan 37,90± 1,36 g. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL).  Perlakuan yang diuji adalah dosis probiotik Rhizopus oryzae terdiri atas T0(kontrol), T1 (penambahan 0,1% probiotik Rhizopus oryzae) dan T2 (penambahan 0,2% probiotik Rhizopus oryzae). Hasil penelitian menunjukkan bahwa pemberian probiotikRhizopus oryzae dalam ransum berpengaruh terhadap total mikroba usus halus dan seka ayam kampung periode grower tetapi berpengaruh terhadap pertumbuhan bobot badan harian ayam kampung umur 0-9 minggu. Pertumbuhan bobot badan harian tertinggi pada perlakuan T1 dan tidak berbeda nyata (P>0,05) terhadap T2 tetapi berbeda nyata terhadap (P<0,05) T0. Kesimpulan penelitian adalah penambahan Rhizopus oryzae dengan dosis 0,1% dan 0,2% pada pakan ayam kampung belum dapat meningkatkan jumlah total mikroba pada usus halus dan seka ayam kampung periode grower, tetapi dapat meningkatkan pertambahan bobot badan harian ayam kampung umur 0-9 minggu dan yang tertinggi pada penambahan dosis 0,1% probiotik Rhizopus oryzae.Kata kunci: Probiotik; fungi;mikroba; produktivitas; ayam kampung ABSTRACT The purpose of this study was to investigate the effect of probiotic Rhizopus oryzaeadministration on the total number of microbes in the smallintestine and caeca of kampoengchickens during the growerperiod. The study was conducted on 11 August to 11 October 2014 in the poultry farm at the Faculty of Animal and Agricultural Sciences, Diponegoro University.The materials used in this study were 100 Day Old Chick (DOC) (unsexed) with initial body weight of 37.90 ± 1,36 g.The experiment was set asCompletely Randomized Design (CRD).The treatments consisted of T0 (control), T1 (0.1% addition of probiotic Rhizopus oryzae to the diet) and T2 (0.2% addition of probiotic Rhizopus oryzae to the diet).The results showed that addition of probioticRhizopus oryzae in the diet did not affect (P>0.05) the total number of microbesin the small intestine and caeca ofchicken during the grower period, but affected (P<0.05) the daily gain of chicken within 0-9 weeks.The highest daily gain was observed in T1 which was not different (P>0.05) from T2 but different (P<0.05) from T0.The conclusion of this study was the administration of Rhizopus oryzae with a dose of 0.1 % and 0.2 % in the chicken dietdid not increase the total number of microbes in the small intestine and caeca of kampong chickensduring the grower period, but the additionof Rhizopus oryzae could increase the daily gain at 0-9 weeks of age,at which the highest impact was observed when Rhizopus oryzae was added at 0.1 %.Keywords: Probiotic; fungi; microbe; productivity;kampoeng chicken
THE DEGREE OF FAT UNSATURATION IN THE TISSUE AND POTENTIAL IMMUNE RESPONSE OF BROILER FED CHLORELLA SP. Sugiharto, Sugiharto; Henckel, P; Lauridsen, C
ANIMAL PRODUCTION Vol 12, No 2 (2010): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

This study was carried out to investigate the effect of Chlorella sp. administered in the diet of broilers on the degree of fat unsaturation, ratio of EPA to AA in the tissue and potential immune response of broiler. A total of 90 heads of one-day-old Ross chicks were assigned in completely randomized design by 3 dietary treatments with 6 repetitions and 5 chicks in each pen. The diets were T1: control (basal diet without enrichment with Chlorella sp.); T2: basal diet enriched with 5-g of Chlorella sp./kg feed; T3: basal diet enriched with 10-g of Chlorella sp./kg feed.  Skinless breast meat was sampled for FA determination at d-36. Chickens were vaccinated at d-6 and d-17 with live vaccine against ND to activate antibodies production, and then 2 ml of blood was collected at d-24 for IgG and IgM quantification. Administration of Chlorella sp. in broiler?s diet had no significant effect on the degree of fat unsaturation, the ratio between EPA and AA contained in the breast muscle and the concentration of IgG and IgM of broiler. In conclusion administration of Chlorella sp. from tropical marine origin in the diet of broiler has no significant effect on the degree of fat unsaturation, ratio of EPA to AA in the tissue, and potential immune response of broiler. The culture temperature in which the Chlorella sp. was cultivated may affect the FA composition of Chlorella sp. (Animal Production 12(2): 96-99 (2010)Abbreviations: AA: arachidonic acid, ALA: ?-linolenic acid, EPA: eicosapentaenoic acid, FA: fatty acids, LA: linoleic acid, PGE2: Prostaglandin E2, PUFA: polyunsaturated fatty acids, SFA: saturated fatty acidKey Words: antibody, broiler, Chlorella sp., fatty acid, PUFA
Effect of decocted turmeric on performance, hematological parameters and carcass traits of broiler chickens Isroli, I.; Yudiarti, T.; Widiastuti, E.; Sugiharto, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 4 (2017): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.4.263-269

Abstract

This study aimed to investigate the effect of decocted turmeric on performance, haematological parameters and carcass traits of broilers. Broiler chicks at 11 days of age were allotted to five groups, including T0 (birds drinking 100% water), T1 (25% decocted turmeric + 75% water), T2 (50% decocted turmeric + 50% water), T3 (75% decocted turmeric + 25% water) and T4 (100% decocted turmeric). The study was arranged based on a completely randomized design (CRD), parameters investigated was performance and blood chemistry (leukocyte, total protein, albumin, globulin, uric acid, glucose). The data were subjected to analysis of variance at 5% probability. Decocted turmeric did not affect weight gain, feed and water consumption and feed conversion ratio of broilers. Lymphocytes proportion was higher (P<0.05) in T1, T2 and T4 than in T0 and T3 birds. The heterophils to lymphocytes ratio was lower (P<0.05) in birds receiving decocted turmeric than control. Globulin concentration and albumin to globulin ratio were higher and lower (P<0.05), respectively, in serum of T2, T3 and T4 than T0 and T1 birds. The proportion of heterophils and concentration of serum total protein, albumin, uric acid and glucose were not different among treatments. The weight of breast meat was higher and lower (P<0.05) in T3 and T4 birds, respectively, compared to the control. In conclusion, providing decocted turmeric through drinking water helped to improve stress responses and increased the weight of breast meat of broiler chickens. 
INFLUENCE OF AGE ON THE SUSCEPTIBILITY OF PIGLETS TO ESCHERICHIA COLI O138:F18 Sugiharto, S.; Lauridsen, C.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 3 (2014): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.3.159-166

Abstract

The ex vivo and in vivo studies were undertaken to examine age-effect on the susceptibility of pigsto E. coli F18-diarrhea. The ex vivo experiment was conducted with 2 and 4 weeks old pigs based on theporcine intestinal organ culture (PIOC) model. The in vivo experiment was performed with 2 and 3weeks old pigs fed milk replacer and inoculated with E. coli F18. E. coli F18 inoculation to the intestinalcultures resulted in higher (P<0.01) counts of E. coli associated to the intestinal tissue, but suchdifference was not observed between the 2 and 4 weeks old pigs. Faecal dry matter (DM) decreased(P=0.01), whereas the proportion of pigs with diarrhea and faecal haemolytic E. coli counts increased(P<0.05) following inoculation, however, the difference between the ages was not observed in the invivo study. The interaction (P=0.01) between age and sampling day was observed for the number of totalwhite blood cells (WBC). The proportion of neutrophils decreased (P=0.01), whereas the lymphocytes,red blood cells (RBC), hemoglobin (Hb) and hematocrit (Hct) increased (P<0.01) after inoculation, butthe differences between age groups were not observed regarding these variables. In conclusion, age (2versus 3 or 4 weeks) did not influence the susceptibility of piglet to E. coli F18-diarrhea.
Effect of dietary supplementation of formic acid, butyric acid or their combination on carcass and meat characteristics of broiler chickens Sugiharto, S.; Yudiarti, T.; Isroli, I.; Widiastuti, E.; Wahyuni, H. I.; Sartono, T. A.; Nurwantoro, N.; Al-Baarri, A. N.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 3 (2019): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.3.286-294

Abstract

The objective of the present study was to evaluate the impact of dietary supplementation of formic acid, butyric acid or their combination on carcass and meat characteristics of broiler chicks. A number of 288 day-old-chicks (Lohmann MB-202 meat broilers) were distributed to four groups, including CONT (basal diet with no additive as a control), FORM (basal diet containing 0.1% formic acid), BUTR (basal diet with 0.03% butyric acid) and FORM+BUTR (basal diet with 0.1% formic and 0.03% butyric acid). At day 35 the birds were slaughtered and eviscerated, and from which the breast meat was obtained. Carcass and cut yields of broilers were not different (P>0.05) among CONT, FORM and BUTR birds. The combination of formic and butyric acids increased (P<0.05) the relative weight of back of broilers. Meat pH value was higher (P<0.05) in organic acids treated than in non-treated birds. Organic acids lowered (P<0.05) drip loss of broiler meat. Butyric acid increased (P<0.05) moisture and decreased (P<0.05) the content of meat protein. Formic acid increased (P<0.05), whereas butyric acid and combination of formic and butyric acids decreased (P<0.05) lightness values of breast meat. Yellowness values of meat increased (P<0.05) with organic acid administration. Intense bands of 25 kDa (phosphoglycerate mutase) was observed in most of meat samples from the treated birds, but not in meat from the control birds. In conclusion, dietary supplementation of organic acids was capable of improving the meat quality of broiler meats. The treatments may prevent the pale, soft and exudative (PSE)-like condition in broiler meats. 
Co-Authors A. Bachtar, Mulni A. Febriana A. Pratama A. Pratama A. R. Pratama A. Setiaji AA Sudharmawan, AA Abbas, Ilham Abdul Chalim, Abdul Adi Pranoto, Adi Adi Pratama, Okky Rizki Aditaruna, Djadjang Adli, Danung Nur Agusetyaningsih, I. Ahmad Zain Sarnoto Akhrima, Nina Al Gifari, Habibulah Ahmad Al-Baarri, Ahmad Ni'matullah Alfani, Muhammad Aliffajaruddin Alfiah Hayati Alfinda Novi Kristanti Alfindo, Bernadito Wildan Aloysius Adya Pramudita Amalia, Sa'adatul Amin, M. Hilman F. Amirudin Amirudin Amrulloh, Amri Anak Agung Istri Sri Wiadnyani Angga, Prayogi Dwina Ani Margawati Anies Setiowati, Anies Anik Setyo Wahyuningsih Anjar Tri Wibowo Anugrah Robby Pratama Anwar Anwar Anwar, Mahfud Aprianto, Boby Arief Firmansyah Aristiyanto, Aristiyanto Arrofi’, Abdullah Dzulfikri Asep Setiaji Atang, Atang Awalya Awalya, Awalya Awan Hariono Azis, T. Abdillah Aziz Syaifudin Mursyid Azizah, Fatiya Nur Azzarah, Regita Asiah B. Sukamto Batubara, Rina Mawardah Beti Kristinawati Bianca, Tanya C. Lauridsen Cahya Setya Utama Ciptaning Wahyu W, Imam Santosa D. A. Lestari Daengs , Achmad Dalmeri Dalmeri Damanik, Muhammad Ridha Syafii Darlani, Darlani Darmawan, Kurnia Sobar Darwin P. Lubis, Darwin Demircan, Turan Desiana Merawati Devanti, Dila Adelia Dewi, Firli RP. Dewi, Rina Dian Farida Asfan Dian Purnomo Dian Wahyu Harjanti Djarot Sugiarso Durubanua, Derita Ramai Dwi Cahyo Kurniawan, Dwi Cahyo E. C. Wulandari E. Kurnianto E. Kusumanti E. Suprijatna, E. E. Widiastuti E. Widiastuti E. Widiastuti E. Widiastuti Efendi Napitupulu Efranita Purba, Desi Marcelina Eko Hariyanto Eko Setio Wibowo Endang Widiastuti Eni Yuniastuti Enung Siti Nurhidayah Eram Tunggul Pawenang Erya, S. N. Evi Widowati F. Wahyono Fadhila, Farah Rosyihana Fahira, Rachyana Farid Farid Agushybana Fatah, Bagus Ari Fatimah Azzahra Fauzi, Ekan Fauzia, Alysya Firdaus, Alif Fitri Laila, Fitri Fitriyono Ayustaningwarno Fortino, Edbert Friantini, Rizki Nurhana Fuadhy Wahyu Fahmi Gilbert Nathaniel Gunawan Gunawan H. I. Wahyuni H. I. Wahyuni H. I. Wahyuni H. I. Wahyuni H. Wahyuni H. Wahyuni H.I. Wahyuni Habibillah, Ahmad Hadida, Vanya Shaquila Hadiningrum, Sri Halim, Jennifer Halleina Rejeki Putri Hartono Hamzah Fansuri Hana Hana, Hana Handari, Miana Ciputra Hanny Indrat Wahyuni Hanny Indrat Wahyuni Harahap, Robby Risky Harjanto, Eko haryani, Desti Haryanto Haryanto Henckel, P Herry Koesyanto Heru Budianto, Bambang Hexa Apriliana Hidayah Hidayat, Mohammad Fatqi Holanda, Stevanny Hutagaol, Ruspiana I. Agusetyaningsih I. Agusetyaningsih I. Agusetyaningsih I. Isroli I. Mangisah I. Mangisah I. Mangisah I. Mareta Ibrahim, Rosida Idris, Muhammad F. Ifada Mufidatul Ula Ihsanuddin, Mokhammad Indri Mareta Ismadi, V. D. Y. B. Ivanna, Julia Jackson Pasini Mairing, Jackson Pasini Jamil, Arniza KM. Jamilah, J. Junaidi Junaidi Jupriadi, Jupriadi Kamariah Tambunan Kardiyanto, Deddy Whinata Karo, Putri Vanessya P. Br Kartinah Kartinah, Kartinah Khairani, Siti Khairul Amri Khoirul Hidayat Kirowati, Dewi Kudhori, Ahmad Kusuma, Dannis Yuda Kusuma, Donny Wira Yudha L. D. Mahfudz L. D. Mahfudz L. Lauridsen Lauridsen, C Lilik Krismiyanto Lim, Jeksen Lisdayana, Nurmalisa Litazkiyyah, Litazkiyyah LUBIS, AMINAH Lukas Lukas Luthfian, F. M. Irfan Mahalul Azam Maharani Ritonga Manalu, Febbry Vebilola Manihuruk, Waldio Manikya Pramudya Mardhatillah, Mardhatillah Marpaung, Jumaylin Br Mateus Sakundarno Adi, Mateus Sakundarno Maulia, Tria Mbina Pinem Muhamad Misbakhul Sholikin Muhammad Azinar MUHAMMAD FAHMI Muhammad Irfan Sulfani Mu’tamar, M Fuad Fauzul N. A. Hasna N.S. Prabowo Nanang Indardi, Nanang Nashruddin, Ahmad Nasrullah Nasrullah Nasuka Nasuka, Nasuka Nawi, Syahruddin Nazila, Nur Alvisyahri Niken Fatimah Nurhayati, Niken Fatimah Ninik Rustanti Novira, Nina Nugroho, Ardi Setyo Nuning Setyaningrum Nur Azizah Nurbulan, Eka Nurcahyani, Dwi Nurhayati, Defi Nurlaila, Lala Nurmuhammad, Riezky Zulfikar Nurwantoro . Nuryanto Nuryanto Oktia Woro Kasmini Handayani Oktriyani, Oktriyani P. Henckel Pasaribu, Rosidah Minta Ito Pawesti, S. D. Pinardi, Janu Prastiwanti Nur Jannah Pratama, A. R. Pratiwi, Kristiani Dina Prihadyatama, Ardila Primasiwi, Asri Pristiwanti, Pristiwanti,N. Priyo Susatyo Probowati, Banun Diyah punding, walter Putera, Shidqi Hamdi Pratama Putri Kartika Sari Putri, Nandila Maysa Putri, Nindy Asyifa Putrinanda, Marcia R. Halimatunnisroh R. Murwani R. Murwani Rahayu, Nika Esti Rahmadi, M Taufik Rahmadi, M. Taufik Rahmadi, Muhammad Taufik Rahmat Winata RAHMAWATI, IKE Rahminingrum, Anita Rakhmawati Rakhmawati Ramadani, Yunda Fitri Ramdes, Muhammad Raza, Muhammad Asif Rezky, Sri Rimba, Fellix Ristanti Pane, Aina Rizki Aprilia Putri Rohani Rohani Rohmatika, Aulia U. Rosyidah, A'liyatur Rosyita, Hafna S. Kismiati S. R. Rini S. Sumarsih S. Sutopo Sahri Sahri Samporno, Kholib Saragi, Emi Juwita Saragih, Rosvilani Sari S, Saurlin Febriana Sari, D. A. P. Sartono, T. A. Sartono, Tri Agus Sembiring, Yolanda Pratiwi Sendi Permana Setiowati, Tutut Setiyono, Eko Setyawan, Dimas Ari Sholikhah, Anindya Mar'atus Shunhaji, Akhmad Sihombing, Evi Ariyanti Marlina Silalahi, Yulianda Ghrace Simagunsong, Tohap Sintong, Mahara Siregar, DELisma Siti Mayang Sari Sitinjak, Sella Oktavia Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sri Sukmaningrum Sri Yunita Suciani, Ayu Sulastri Sulastri Suparman Suparman Supriyadi Supriyadi Supriyanto Supriyanto Suroso Suroso Suryaningsih, Suhestri Susena, Satrio Jaya Suyana, Nana Swandana, Atika Syahbana, Fadil Ali Syarifah Syarifah, Syarifah T. A. Sartono T. A. Sartono T. A. Sartono T. Ayaşan T. Sartono T. Sartono T. Yudiarti T. Yudiarti T. Yudiarti Tandiyo Rahayu Tanjung Puspa Maldini Taufiq Hidayah Titi Chasanah Trisna Ayu Oktavia Tupan Tupan Turrini Yudiarti Utami, Fitra Isna V. D. Yunianto Vanda, Vanda Vinami Yulian W. Winarti Wardani, Aulia Wibawa, Koerniawan Dwi Widiastuti, Endang Wijayanti, Ely Tri Wijayanti, Gratiana Ekaningsih Wijinindyah, Ayutha Wildana Latif Mahmudi, Wildana Latif Wildansyah lubis, Wildansyah William William Windiarini Lestari Wita Oktaviana Wiwiek Natalya Yogi Putranto Yosephine Sri Wulan Manuhara Yuliana, Afid Yupi Royani Yupito, Yupito Zam, Mahalul