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All Journal AGROEKOTEKNOLOGI Biology, Medicine, & Natural Product Chemistry
Beatrix Sofranes Napitupulu
University Of Sumatera Utara
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

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Effect of coconut water concentration and the duration of true shallot seed soaking on growth and production of shallot (Allium ascalonicum L.) of Tuk-Tuk varieties Beatrix Sofranes Napitupulu; Ratna Rosanty Lahay; Asil Barus
Jurnal Agroekoteknologi Vol 6, No 4,Okt (2018)
Publisher : Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.311 KB) | DOI: 10.32734/jaet.v6i4,Okt.19148

Abstract

This research was conducted to find out the effect of coconut water concentration and the duration of true shallot seed soaking on the growth and production of shallot. This research was conducted on community farm at Jalan Bunga Rampai II Gang Saudara, Kelurahan Simalingkar B, Kecamatan Medan Tuntungan, Medan, began from April to August 2017. The research used a Factorial Randomized Block Design with 2 treatment factors: coconut water concentration at 5 levels: 0; 25; 50; 75; 100 %, and duration of seed soaking at 3 levels: 60; 120; 180 minutes. The parameters observed were number of plant height at 2 to 7 weeks after transplanting (WAT), bulb diameter, and dry bulb weight. The result of this research showed that coconut water concentration had no significant effect on all observation parameter. The duration of seed soaking treatment had significant effect on plant height and dry bulb weight. The highest dry bulb weight was obtained at 120 minutes of seed soaking. The interaction of coconut water concentration and the duration of true shallot seed soaking treatment had no significant effect on all observation parameters.Keywords: coconut water concentration, duration of seed soaking, true shallot seed
Isolation, Morphological Characterization, Biochemistry, and Antagonistic Activity of Endophytic Bacteria from Rice Against Xanthomonas oryzae pv. Oryzae Saipul Sihotang; Alfons Dwi Putra Butar-Butar; Lita Nasution; Haliatur Rahma; Trizelia Trizelia; Magdalena Saragih; Retna Astuti Kuswardani; Ika Listiana; Beatrix Sofranes Napitupulu
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.825-829

Abstract

Endophytic bacteria have the potential as environmentally friendly biocontrol agents in controlling bacterial leaf blight (BLB) in rice caused by Xanthomonas oryzae pv. oryzae (Xoo). This study aims to isolate, characterize morphology and biochemical tests, and test the antagonistic activity of endophytic bacteria from rice against Xoo. Isolation was carried out from healthy rice root tissue using a surface sterilization method. Morphological characterization included colony shape, color, edge, elevation, and Gram properties, while biochemical characterization included catalase, oxidase, motility, starch hydrolysis, and sugar fermentation tests. The antagonistic test used a dual culture assay method on NA (nutrient agar) media. The isolation results obtained six endophytic bacterial isolates (BETP 01–06) identified as the genus Pseudomonas and Bacillus with varying colony morphological characters. Antimicrobial activity test showed two potential isolates, BETP 03 (Pseudomonas) and BETP 05 (Bacillus), with an average inhibitory diameter of 16.3 mm and 16.5 mm against Xoo, respectively. Biochemical characterization showed that BETP 03 was bacillus, Gram-negative, citrate-negative, motile, gelatin hydrolysis positive, starch hydrolysis positive, and catalase positive; while BETP 05 was bacillus, Gram-positive, citrate-positive, motile, gelatin hydrolysis negative, starch hydrolysis positive, and catalase positive. These results indicate that both isolates have high potential as biological biocontrol agents in controlling bacterial leaf blight disease in rice.
Co-Authors Alfons Dwi Putra Butar-Butar Asil Barus Haliatur Rahma Ika Listiana Lahay, Ratna Rosanty Lita Nasution Magdalena Saragih Retna Astuti Kuswardani Saipul Sihotang Trizelia Trizelia