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All Journal Jurnal Ilmu Pertanian Indonesia Biology, Medicine, & Natural Product Chemistry BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ilmiah Pertanian ( JIPERTA) Journal Liaison Academia and Society
Saipul Sihotang, Saipul
Universitas Medan Area
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STIMULASI TUNAS PISANG BARANGAN (Musa acuminata L.) SECARA IN VITRO DENGAN BERBAGAI KONSENTRASI IBA (Indole-3-butyric acid) DAN BA (Benzyladenin) Sihotang, Saipul
BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Vol 3, No 1 (2016): Agustus
Publisher : Program Studi Biologi, Fakultas Biologi Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.776 KB)

Abstract

The research aim was get the best medium combination of IBA dan BA to stimulated formation number of shoots of banana Barangan. The study used factorial experiment aranged in Completely Randomized Desigh (CRD). The treatment consisted of 2 factors. The frist factor was the IBA concentration, consisted of three levels 0,0 mg/l, 0,5 mg/l, and 1,0 mg/l. The second factor was BA concentration consisted of four level, 0,0 mg/l, 1,5 mg/l, 3,0 mg/l, and 4,5 mg/l. The result showed that combinations of IBA 0,0 mg/l and BA 0,5 mg/l was the best medium to induce shoot multiplication with 4,00 shoots explant. IBA concentrations had no significant effect on number of shoots. However, the IBA concentation affected the number of root explant.
Induksi Kalus dengan BAP (Benzylaminopurin) dan IAA (Indoleacetic acid) pada Tanaman Cabai (Capsicum annuum L.) Lokal Genotipe Lotanbar Sumatera Barat Sihotang, Saipul; Renfiyeni, Renfiyeni; Suliansyah, Irfan; Jamsari, Jamsari
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol 3, No 2 (2019): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Juni
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1129.053 KB) | DOI: 10.31289/agr.v3i2.2547

Abstract

This study was aimed to obtain the method in the study of callus induction of in vitro.  The research activities were carried out at Laboratory of Biotechnology, Faculty of Agrotechnology, Andalas University. This research used decriptive and experimental methods. One-way ANOVA analysis followed by Duncan’s test was used to determine significant differences (P ≤ 0.05). All statistical analysis were performed using the SPSS Ver. 23 statistical software package. The results showed that medium of callus induction is 4,0 mg/L BAP + 0,5 mg/L IAA with formation of callus (days), and average of callus formed, namely 4,33 days,  and 9,67 callus.
Induksi Kalus dengan BAP (Benzylaminopurin) dan IAA (Indoleacetic acid) pada Tanaman Cabai (Capsicum annuum L.) Lokal Genotipe Lotanbar Sumatera Barat Sihotang, Saipul; Renfiyeni, Renfiyeni; Suliansyah, Irfan; Jamsari, Jamsari
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol 3, No 2 (2019): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Juni
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v3i2.2547

Abstract

This study was aimed to obtain the method in the study of callus induction of in vitro.  The research activities were carried out at Laboratory of Biotechnology, Faculty of Agrotechnology, Andalas University. This research used decriptive and experimental methods. One-way ANOVA analysis followed by Duncan’s test was used to determine significant differences (P ≤ 0.05). All statistical analysis were performed using the SPSS Ver. 23 statistical software package. The results showed that medium of callus induction is 4,0 mg/L BAP + 0,5 mg/L IAA with formation of callus (days), and average of callus formed, namely 4,33 days,  and 9,67 callus.
Variety Study on Germination Power of Palm Seeds (Elaeis guineensis Jacq.) Yabani, Yabani; Kuswardani, Retna Astuti; Susanto, Agus; Sihotang, Saipul
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i3.5913

Abstract

Seeds as planting material play an important role in agricultural development. The high demand for oil palm sprouts causes the long germination time to become an obstacle for consumers and seed producers. The aim of this research is to examine varieties on germination capacity of oil palm seeds. Research was conducted at the PPKS Seed Processing Unit on nine PPKS varieties (Simalungun, PPKS 540, Yangambi, PPKS 239, PPKS 718, Dumpy, Avros, Langkat, and PPKS 540 NG). The parameter observed was the germination power (DB) of the seeds. The treatment given in the germination process for all varieties is carried out to ensure that the environment is favorable for germination (according to the SOP). Observation results showed that PPKS 540 variety had the highest DB (84.9%), followed by Simalungun (81.46%), PPKS 540 NG (75.46%), Langkat (75.43%), Dumpy (73.41 %), PPKS 718 (72.29), Yangambi (68.81%), PPKS 239 (65.74%), and Avros (56.92%). This is thought to be due to the influence of internal factors, related to the conditions of the germinated seeds, both genetics and hormones, as well as external conditions which include optimal water, temperature and oxygen treatment during germination. The results show that the water content (KA) of the seeds in the soaking period (I) was 16-20%, and soaking (II) was 19-22%, all processed seeds were still at a safe level of water content for the process of breaking dormancy of oil palm seeds. The characteristics of parent tree of the seed variety are the cause of differences in seed germination
Study on the Production of Superior Seed Bunches of Oil Palm (Elaeis guineensis Jacq.) (Case Study: Indonesian Oil Palm Research Institute-PPKS Marihat Unit) Yabani, Yabani; Kuswardani, Retna Astuti; Susanto, Agus; Sihotang, Saipul
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i2.5882

Abstract

This research aims to examine the production of superior oil palm seed bunches in the PPKS Marihat Unit.Data and information collection is carried out using direct and indirect methods. Field observations carried out include: Area conditions including: topography, soil conditions, Plant conditions including: plant growth, plant population and fruit condition, Technical culture conditions: fertilization, harvesting of seed bunches. Supporting data collected includes: garden production data, rainfall and fertilization data. The research results are bSeveral factors that cause low production include: a) fertilizer amounts that are far from plant needs, given the time, type; b) plant density which is classified as less than optimal due to ganoderma attacks. The number of bunches is the result of plant physiological processes, mainly determined by the ecophysiological conditions of the plant around 24 months before harvest, where the decrease in production in the number of bunches harvested in 2020 is the result of ecophysiological conditions in the form of fertilization which is not appropriate in 2019-2020.
The Combination of Plant Growth Promoting Rhizobacteria (PGPR) Paitan Root with Several Types of Bokashi and Its Effect on Tatsoi Meyuliana, Aulia; Yora, Muharama; Elinda, Friza; Miranda, Cut Nyak Dara; Sihotang, Saipul
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol. 9 No. 1 (2024): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, 9 (1)Desember 2024
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v9i1.12957

Abstract

The combination of PGPR (Plant Growth Promoting Rhizobacteria) with bokashi aims to increase nutrient availability in the soil. PGPR which comes from paitan roots is known to have the potential to help nutrient availability so by combining it with bokashi it is hoped that it will optimize its potential in increasing tatsoi production. The bokashi used in this research were chicken manure bokashi and goat manure bokashi. Research design with two factors, namely five doses of PGPR and two types of bokashi. The PGPR doses used were 0 ml/L (D0), 50 ml/L (D1), 100 ml/L (D2), 150 ml/L (D3), 200 ml/L (D4) and two types of Chicken bokashi (A) and Goat Bokashi (K) with 5 repetitions. The observations measured were plant height (cm), number of leaves (strands) and crop diameter (cm). The results of the research showed that the combination of PGPR with bokashi chicken manure and bokashi goat manure did not have a significant effect on plant growth, namely plant height, number of leaves and crop diameter of tatsoi.
Characteristics of Wild Edible Mushroom Knowledge of Mushroom Foragers in Indonesia Sihotang, Saipul; Hermawan, Rudy; Putra, Ivan Permana
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 2 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.2.368

Abstract

Edible wild mushrooms (EWM) are food sources which have high nutritional value. Although they are not the main choice of daily foods in Indonesia, various local communities and mushroom foragers have been consuming EWM for decades, yet without proper documentation. This study aimed to preserve the knowledge of EWM consumption to increase the popularity and public awareness of EWM as alternative food in Indonesia. Data was collected via a structural survey to 50 mushroom hobbyists with range of 18-50 years old and representing several ethnic groups in Indonesia: Javanese, Malay, Sundanese, Banten, and Bugis. Most of the respondents have consumed EWM for more than 5 years, with a frequency of consuming more than once per month, and the knowledge of foraging was obtained from their families. The edible mushrooms were collected mostly after every January and usually consumed by themselves. The location for mushroom seeking and species of EWM mostly were the forests around residence area and Termitomyces spp. (local names: ‘jamur barat’, ‘jamur bulan’, ‘jamur rayap’, ‘jamur trucuk’, ‘jamur sempagi’, respectively). The taste of mushroom was the main reason for forage activity among the hobbyists. They generally consumed all parts of basidiomata and preferred the soft texture of fruiting body. The soup dish was the most preferred by foragers, in contrast to fried cooking type. Most of them distinguish EWM from poisonous mushrooms by observing certain structures basidiomata, however, cases of wild mushroom poisoning have occurred among foragers with low occurrence of frequency. Keywords: Indonesia; edible wild mushrooms; characteristics; mushroom foragers
Pengaruh Mikoriza dan POC Urin Sapi terhadap Pertumbuhan Cabai Merah di Zona Iklim Kering E Parlindungan Sitepu, David; Sihotang, Saipul
Jurnal Ilmiah Pertanian (JIPERTA) Vol 7, No 2 (2025): Jurnal Ilmiah Pertanian (JIPERTA), September
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jiperta.v7i2.6009

Abstract

Red chili pepper (Capsicum annuum L.) is a horticultural commodity with high economic value that is greatly influenced by agroclimatic conditions. In dry climate zone E (Oldeman classification), plant growth is often hampered due to water limitations and low soil fertility. This study aims to evaluate the effect of arbuscular mycorrhizal administration and liquid organic fertilizer (POC) of cow urine on the vegetative growth of red chili plants in dry climate zone E. The study was conducted using a factorial Random Group Design (RAK) with two factors: mycorrhizal dose (0, 35, 85, and 135 g/plant) and POC concentration of cow urine (0, 30, 35, and 40 ml/L), Each was repeated three times, making a total of 48 experimental units. The parameters observed included plant height, number of leaves, stem diameter, time of emergence and number of shoots. The results showed that the dose of mycorrhizal 135 g and the POC concentration of 40 ml/L gave the best results on almost all vegetative parameters. The interaction of the two also showed a significant influence on plant height and number of shoots. These findings support the application of bioagent-based eco-friendly technologies in sustainable farming systems on dry climates.
Potensi Aplikasi Endofit dalam Agroekosistem Berkelanjutan: Biofertilizer dan Biokontrol Hayati Sihotang, Saipul; Panggabean, Ellen Lumisar; Alifyah, Nabila; Saragih, Nur Betty; Nasution, Lita; Halawa, Emmanuel; Saragih, Ryan
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol. 9 No. 2 (2025): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, 9 (2) Juni 2025
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v9i2.7859

Abstract

Endophytic microorganisms have become a central focus in numerous studies related to sustainable agriculture due to their dual potential as biological agents—functioning both as biocontrol and biofertilizers. The presence of endophytes within plant tissues has been shown to enhance vegetative growth, strengthen resistance against pathogenic attacks, and optimize nutrient uptake. This article presents a systematic review of recent studies from 2018 to 2024, addressing the classification, mechanisms of action, and potential applications of endophytic microorganisms in agriculture. Several bacterial genera, including Bacillus spp., Azospirillum spp., and Pseudomonas spp., along with non-pathogenic fungal groups such as Trichoderma spp. and Fusarium spp., have demonstrated effectiveness in nitrogen fixation, phosphate solubilization, phytohormone synthesis, and the production of antimicrobial compounds. Research findings indicate that the application of endophytic microorganisms as biological inputs holds great promise in supporting the development of environmentally friendly, sustainable, and high-yielding agricultural systems.
Biofertilizer For Increasing Growth And Yield Of Melon (Cucumis Melo L.) Alifyah, Nabila; Betty Saragih, Nur; Maulana, Bagus; Sihotang, Saipul
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol. 9 No. 2 (2025): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, 9 (2) Juni 2025
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v9i2.12584

Abstract

This study aims to develop biofertilizer from Metharizium sp. to increase the production of melon plants (Cucumis melo L.). The excessive use of chemical fertilizers has damaged soil quality and the environment in Indonesia. Environmentally friendly biofertilizers can be a solution to improve soil fertility and increase crop yields. The research was conducted in Serdang Bedagai Regency from April to July 2024. The research method includes land preparation, planting, making granular compost, fertilizer application, and data analysis. The study is expected to provide an effective Metharizium sp. granular fertilizer formulation to increase melon production and reduce the use of chemical fertilizers.
Co-Authors Alifyah, Nabila Betty Saragih, Nur Butar-Butar, Alfons Dwi Putra Elinda, Friza Ginting, Rajiv Halawa, Emmanuel Haliatur Rahma, Haliatur Ida Ayu Putu Sri Widnyani Ika Listiana Irfan Suliansyah Ivan Permana Putra Jamsari Jamsari Maulana, Bagus Meyuliana, Aulia Miranda, Cut Nyak Dara Mochammad Imron Awalludin Napitupulu, Beatrix Sofranes Nasution, Lita Pandiangan, Indera Prabowo Panggabean, Ellen Lumisar Parlindungan Sitepu, David Renfiyeni Retna Astuti Kuswardani Rudy Hermawan Saragih, Magdalena Saragih, Nur Betty Saragih, Ryan Sembiring, Riduan Sembiring, Swati Silaban, Sri Rejeki Trizelia . Yabani, Yabani Yora, Muharama