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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Biology, Medicine, & Natural Product Chemistry BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Penelitian Pendidikan IPA (JPPIPA) Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Jurnal Pembelajaran dan Biologi Nukleus Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Ilmiah Pertanian ( JIPERTA) Journal of Applied Agricultural Science and Technology Jurnal Natural Journal Liaison Academia and Society
Saipul Sihotang
Universitas Medan Area
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STIMULASI TUNAS PISANG BARANGAN (Musa acuminata L.) SECARA IN VITRO DENGAN BERBAGAI KONSENTRASI IBA (Indole-3-butyric acid) DAN BA (Benzyladenin) Sihotang, Saipul
BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Vol 3, No 1 (2016): Agustus
Publisher : Program Studi Biologi, Fakultas Biologi Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.776 KB)

Abstract

The research aim was get the best medium combination of IBA dan BA to stimulated formation number of shoots of banana Barangan. The study used factorial experiment aranged in Completely Randomized Desigh (CRD). The treatment consisted of 2 factors. The frist factor was the IBA concentration, consisted of three levels 0,0 mg/l, 0,5 mg/l, and 1,0 mg/l. The second factor was BA concentration consisted of four level, 0,0 mg/l, 1,5 mg/l, 3,0 mg/l, and 4,5 mg/l. The result showed that combinations of IBA 0,0 mg/l and BA 0,5 mg/l was the best medium to induce shoot multiplication with 4,00 shoots explant. IBA concentrations had no significant effect on number of shoots. However, the IBA concentation affected the number of root explant.
Induksi Kalus dengan BAP (Benzylaminopurin) dan IAA (Indoleacetic acid) pada Tanaman Cabai (Capsicum annuum L.) Lokal Genotipe Lotanbar Sumatera Barat Sihotang, Saipul; Renfiyeni, Renfiyeni; Suliansyah, Irfan; Jamsari, Jamsari
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol 3, No 2 (2019): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Juni
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1129.053 KB) | DOI: 10.31289/agr.v3i2.2547

Abstract

This study was aimed to obtain the method in the study of callus induction of in vitro.  The research activities were carried out at Laboratory of Biotechnology, Faculty of Agrotechnology, Andalas University. This research used decriptive and experimental methods. One-way ANOVA analysis followed by Duncan’s test was used to determine significant differences (P ≤ 0.05). All statistical analysis were performed using the SPSS Ver. 23 statistical software package. The results showed that medium of callus induction is 4,0 mg/L BAP + 0,5 mg/L IAA with formation of callus (days), and average of callus formed, namely 4,33 days,  and 9,67 callus.
Induksi Kalus dengan BAP (Benzylaminopurin) dan IAA (Indoleacetic acid) pada Tanaman Cabai (Capsicum annuum L.) Lokal Genotipe Lotanbar Sumatera Barat Sihotang, Saipul; Renfiyeni, Renfiyeni; Suliansyah, Irfan; Jamsari, Jamsari
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol 3, No 2 (2019): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Juni
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v3i2.2547

Abstract

This study was aimed to obtain the method in the study of callus induction of in vitro.  The research activities were carried out at Laboratory of Biotechnology, Faculty of Agrotechnology, Andalas University. This research used decriptive and experimental methods. One-way ANOVA analysis followed by Duncan’s test was used to determine significant differences (P ≤ 0.05). All statistical analysis were performed using the SPSS Ver. 23 statistical software package. The results showed that medium of callus induction is 4,0 mg/L BAP + 0,5 mg/L IAA with formation of callus (days), and average of callus formed, namely 4,33 days,  and 9,67 callus.
STIMULASI TUNAS PISANG BARANGAN (Musa acuminata L.) SECARA IN VITRO DENGAN BERBAGAI KONSENTRASI IBA (Indole-3-butyric acid) DAN BA (Benzyladenin) Saipul Sihotang
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 3, No 1 (2016): Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v3i1.808

Abstract

The research aim was get the best medium combination of IBA dan BA to stimulated formation number of shoots of banana Barangan. The study used factorial experiment aranged in Completely Randomized Desigh (CRD). The treatment consisted of 2 factors. The frist factor was the IBA concentration, consisted of three levels 0,0 mg/l, 0,5 mg/l, and 1,0 mg/l. The second factor was BA concentration consisted of four level, 0,0 mg/l, 1,5 mg/l, 3,0 mg/l, and 4,5 mg/l. The result showed that combinations of IBA 0,0 mg/l and BA 0,5 mg/l was the best medium to induce shoot multiplication with 4,00 shoots explant. IBA concentrations had no significant effect on number of shoots. However, the IBA concentation affected the number of root explant.
Intercropping system of Capsicum annum L. and Tagetes erecta with Mycorrhizal application and cow waste compost SUSWATI SUSWATI; SERI DEPI; SAISA SAISA; SITI MARDIANA; SAIPUL SIHOTANG
Jurnal Natural Volume 22 Number 3, October 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1431.608 KB) | DOI: 10.24815/jn.v22i3.25530

Abstract

Factors that cause fluctuations in chili production are nutrient content and attack by plant disturbing organisms (PDO).  To overcome pest attacks can be done by planting refugia which serves as an alternative that will bring in natural enemies.  In addition, the application of cow dung and mycorrhizal compost as biological agents will be able to maintain soil quality and plant growth.  This study aims to determine the effect of cow waste compost and arbuscular mycorrhizal fungi (AMF) on the growth and production of red chili plants intercropped with refugia plants.  The method was a factorial randomized block design consisting of 2 factors, namely: cow waste compost and AMF.  Cow waste compost consists of 5 levels, namely: K0 = without giving cow waste compost, K1 = cow waste compost at a dose of 5 tons/ha-1, K2 = cow waste compost at a dose of 10 tons/ha-1, K3 = cow waste compost at a dose 15 tons/ha-1, K4= cow waste compost dose of 20 tons/ha-1.  Meanwhile, arbuscular mycorrhizal fungi consisted of 4 levels, namely: M0= without AMF, M1= AMF 50 kg/ha-1, M2= AMF 100 kg/ha-1, M3= AMF 150 kg/ha-1.  The results showed that AMF had a most significant effect on the number of fruits per plant, production per plant, and production per plot, but not significantly different on plant height, some branches, and flowering age of Laris variety red chili.  AMF treatment with 150 kg/ha (M3) dose had the highest average value for all observed parameters.
EDUKASI DAN PENYULUHAN HIDUP BERSIH DAN SEHAT PADA GURU-GURU LPIT NURUL ILMI PASCA COVID 19 Saipul Sihotang; Ellen Lumisar Panggabean; Sartini Sartini; Retna Astuti Kuswardani
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 4 No. 2 (2023): Volume 4 Nomor 2 Tahun 2023
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v4i2.12939

Abstract

Perilaku hidup bersih dan sehat harus dilaksanakan oleh setiap warga masyarakat. Namun, tidak semua anggota masyarakat terutama anak-anak di Sekolah Nurul Ilmi mempunyai pengetahuan dan kesadaran untuk melakukan hidup bersih dan sehat pasca Covid-19. Tujuan dari pengabdian ini adalah untuk meningkatkan pengetahuan dan kesadaran kepada Guru-guru NURUL ILMI tentang pentingnya menjaga hidup bersih dan sehat selama pasca Covid-19. Metode yang digunakan dalam kegiatan ini adalah metode ceramah, dan praktek. Peserta dari pengabdian ini berjumlah 40 peserta yang terdiri dari guru-guru, dan petugas kebersihan di NURUL ILMI. Hasil pengabdian menyatakan bahwa terjadi peningkatan pengetahuan dan kesadaran pada guru-guru dan petugas kebersihan mengenai hidup bersih dan sehat pasca Covid-19. Dengan adanya kegitan pengabdian masyarakat ini diharapkan mneumbuhkan rasa kepedulian terhadap sesame dan perlunya menjaga kesehatan pasca covid-19
Isolasi Bakteri Endofit Pada Daun Terong Ungu (Solanum melongena L.) Saipul Sihotang; Merlan Manurung; Emmanuel Halawa; Imam Alfazri; Natalina Tarigan; Frisky Purba; Yosafat Siregar; Muhammad Aldy
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol. 7 No. 2 (2023): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Juni
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v7i2.10005

Abstract

Penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit yang terdapat pada daun terong (Solanum melongena) dan mengetahui kemampuannya untuk meningkatkan produktivitas pada terong. Penelitian ini menggunakan metode eksperimen berskala laboratorium. Hasil isolasi dari daun terong Solanum melongena ditemukan dua koloni isolat yang berbeda yang diberi dengan kode isolat A1 dan A2. Kedua isolat bakteri endofit diduga merupakan genus Bacillus. Hasil kedua isolat menyatakan bahwa bakteri endofit tersebut mampu meningkatkan Produktivitas hasil tanaman terong.
An Introduction to Indonesian Wild Shiitake Ivan Permana Putra; Mada Triandala Sibero; Saipul Sihotang; Lilis Supratman; Rudy Hermawan; Oktan Dwi Nurhayat
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1132-1138

Abstract

Pegler suggested that shiitake comprises three morphological species: Lentinula edodes (continental and northeast Asia), L. lateritia (tropical Asia and Australasia), and L. novae-zelandiae (New Zealand). The current study reported for the first time the occurrence of L. lateritia (Berk.) Pegler in Indonesia. During a fungus foray in Kerinci (Jambi, Sumatra, Indonesia) in 2022 and 2023 by the Indonesian mushroom hunter community, some basidiomata of Lentinula were obtained. At a glance, our specimens resembled L. edodes. The current study aims to justify the taxonomical position of our specimens based on morphological and molecular data. The fresh basidiomata were used for morphological and molecular analyses. The molecular work was done using ITS 4/5 Primers for phylogenetic analysis of rDNA-ITS region. Morphologically, the uniformly reddish brown, smooth, and glabrous of pileus confirmed our specimens as L. lateritia. In addition, the absence of a range of colors and squamules pileus distinguished L. lateritia BO24628 form L. edodes, while the formation of florets cheilocystidia in L. madagasikarensis was the distinctive character of our specimens. The BLAST result revealed that our specimen has high similarity (99-100%) with L. lateritia and L. edodes as the top hits. The phylogenetic tree (RAxML) nested our specimens in the L. lateria clade and is closely related to one specimen from Papua New Guenia (PNG) (BS 98%). In addition, L. lateritia BO24628 has a sister clade of the specimen from PNG and Australia. Moreover, we provide the herbarium collection of wild L. lateritia in Indonesia.
PELATIHAN HIDROPONIK UNTUK MENUMBUHKAN KESADARAN URBAN FARMING SISWA SDN 105267 SEI MENCIRIM KECAMATAN SUNGGAL KABUPATEN DELI SERDANG Saipul Sihotang; Zulheri Noer; Asmah Indrawati
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 4 No. 4 (2023): Volume 4 Nomor 4 Tahun 2023
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v4i4.18706

Abstract

Kegiatan ini bertujuan untuk memberikan pengetahuan tentang budidaya sayuran dengan cara hidroponik, jenis tanaman yang dapat dibudidayakan secara hidroponik, dan cara menyiapkan media tanam secara hidroponik bagi guru dan pendamping, siswa-siswi SDN 105267 Sei Mencirim serta Bapak/Ibu orang tua siswa dan memberikan pemahaman pentingnya menanam sayuran sebagai kebutuhan keluarga, dan menjaga kesehatan bahan pangan. Kegiatan Pengabdian Kepada Masyarakat ini bertujuan untuk: 1) Memberikan pengetahuan dan praktek tentang teknik budidaya tanaman hidroponik, jenis tanaman yang dapat dibudidayakan secara hidroponik, dan cara menyiapkan media tanam secara hidroponik di SDN 105267 Sei Mencirim; 2) Memberikan pemahaman pentingnya menanam sayuran dengan memanfaatkan lahan sempit dan menjaga kesehatan bahan pangan. Kegiatan pelatihan dengan praktek melalui PPM ini diharapkan bermanfaat bagi mitra dalam : 1) Memberikan keterampilan kepada guru, siswa dan sebagian orang tua siswa untuk memanfaatkan waktu luang dan lahan; 2) Memberikan bekal kepada guru, siswa dan sebagian orang tua siswa berbagi keterampilan pada masyarakat di sekitarnya; 3. Transformasi baru dalam pemanfaatkan lahan sempit dan limbah botol plastik bekas, dalam jangka pendek akan meningkatkan penghasilan dan berkurangnya angka penganguran didesa tersebut. Kegiatan ini dilaksanakan di Sekolah SDN 105267 Sei Mencirim, lokasi Perguruan Tinggi dengan mitra adalah 35 km, adapun yang ikut terlibat dalam kegitan ini adalah dengan kelompok sasaran guru dan pendamping, siswa-siswi dan sebagian orangtua siswa dengan jumlah peserta sebanyak 60 orang, 3 dari dosen pengabdi dan 2 mahasiswa. Adapun pelaksanaan kegiatan ini melalui ceramah, demonstrasi, dan praktek individu. Hasil kegiatan yang sudah dilaksanakan antara lain, introduksi metode dan teknik instalasi hidroponik, pembenihan dan pemeliharaan sayuran, pengetahuan alat dan bahan, dan cara produksi pupuk organik serta aplikasinya. Program pengabdian masyarakat yang sudah dilaksanakan ini mampu meningkatkan wawasan dan kemampuan terkait budidaya sayuran secara hidroponik, dan transformasi baru dalam budidaya sayur dilahan sempit.
Intercropping system of Capsicum annum L. and Tagetes erecta with Mycorrhizal application and cow waste compost SUSWATI SUSWATI; SERI DEPI; SAISA SAISA; SITI MARDIANA; SAIPUL SIHOTANG
Jurnal Natural Volume 22 Number 3, October 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v22i3.25530

Abstract

Factors that cause fluctuations in chili production are nutrient content and attack by plant disturbing organisms (PDO).  To overcome pest attacks can be done by planting refugia which serves as an alternative that will bring in natural enemies.  In addition, the application of cow dung and mycorrhizal compost as biological agents will be able to maintain soil quality and plant growth.  This study aims to determine the effect of cow waste compost and arbuscular mycorrhizal fungi (AMF) on the growth and production of red chili plants intercropped with refugia plants.  The method was a factorial randomized block design consisting of 2 factors, namely: cow waste compost and AMF.  Cow waste compost consists of 5 levels, namely: K0 = without giving cow waste compost, K1 = cow waste compost at a dose of 5 tons/ha-1, K2 = cow waste compost at a dose of 10 tons/ha-1, K3 = cow waste compost at a dose 15 tons/ha-1, K4= cow waste compost dose of 20 tons/ha-1.  Meanwhile, arbuscular mycorrhizal fungi consisted of 4 levels, namely: M0= without AMF, M1= AMF 50 kg/ha-1, M2= AMF 100 kg/ha-1, M3= AMF 150 kg/ha-1.  The results showed that AMF had a most significant effect on the number of fruits per plant, production per plant, and production per plot, but not significantly different on plant height, some branches, and flowering age of Laris variety red chili.  AMF treatment with 150 kg/ha (M3) dose had the highest average value for all observed parameters.
Co-Authors Alifyah, Nabila Angga Ade Sahfitra Asmah Indrawati Asmah Indrawaty, Asmah Azwana Azwana Betty Saragih, Nur Butar-Butar, Alfons Dwi Putra Elinda, Friza Ellen Lumisar Panggabean Elli Afrida Emmanuel Halawa Erwin Pane, Erwin Frisky Purba Ginting, Rajiv Halawa, Emmanuel Haliatur Rahma, Haliatur Hasibuan, Sayyid Al-Fadhil Herliyani, Selvi Hutapea, Sumihar Ida Ayu Putu Sri Widnyani Ika Listiana Imam Alfazri Irfan Suliansyah Ivan Permana Putra Jamsari Jamsari Lilis Supratman Mada Triandala Sibero Manik, Prendy Jonringga Mariana, Merlyn Maulana, Bagus Merlan Manurung Meyuliana, Aulia Miranda, Cut Nyak Dara Mochammad Imron Awalludin Muhammad Aldy Napitupulu, Beatrix Sofranes Nasution, Lita Natalina Tarigan Nur Asyiah Dalimunthe Oktan Dwi Nurhayat Pandiangan, Indera Prabowo Panggabean, Ellen L. Parlindungan Sitepu, David Qohar, Adi Fathul Rahmiati Rahmiati Renfiyeni Retna Astuti Kuswardani Riyanto Riyanto Rizal Aziz, Rizal Rudy Hermawan Rudy Hermawan SAISA SAISA Saragih, Magdalena Saragih, Nur Betty Saragih, Ryan Sartini Sartini Sembiring, Riduan Sembiring, Swati SERI DEPI Sianturi, Parulian Sihombing, Roni Paulus Silaban, Sri Rejeki Siregar, Rahma Sari Siti Mardiana SITI MARDIANA Sitompul, Marco Y. F Suswati Suswati Suswati Tanjung, Denny Akbar Trizelia . Yabani, Yabani Yanty, Dini Puspita Yora, Muharama Yosafat Siregar Zulheri Noer