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Lies Indah Sutiknowati
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MICROBES PARAMETERS OF WATER QUALITY FOR AQUACULTURE ON PARI ISLAND WATERS Lies Indah Sutiknowati
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 5 No. 1 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.248 KB) | DOI: 10.29244/jitkt.v5i1.7769

Abstract

The objective of this research was to evaluate waters quality in Pari island waters for aquaculture purpose based on bacteriological information conducted in Mei and September 2011. Microbiological parameters analyzed were total density of bacteria for coliforms, E.coli, pathogenic, heterotrophic, halotoleran, phosphate-nitrate-ammonia breaker, and total cells. Method to analyze coliform bacteria was filtration, identification of pathogenic bacteria using biochemical test, density analises for heterotrophic bacteria, analises for phosphate-nitrate-ammonia breaker bacteria using pour plate, and total cell using Acridine Orange Epifluorescence Microscopy. Results showed that the abundance of total coliform cell was about 1000-7000 colony forming unit (cfu)/100 ml. The abundance of heterotrophic, halotolerant, phosphate-nitrate-ammonia bacteria in seawater was (3.6-4.3)x105 cfu/ml, (1.1-1.3)x105 cfu/ml, (0.5-3.44)x103cfu/ml; and (3.6-6.7)x105 cfu/ml, (1.6-2.7)x105 cfu/ml, (0.6-5.22)x103 cfu/ml in sediment, respectively. The total cell of bacteria was (0.05-2.1)x107cells/ml. The dog-conch (Strombus turturella) and blood-clamps (Anadara granosa) can survive in Pari Island and there was a significant increase in sea grass litter with growth average of 0.67 mm/day and 0.90 mm/day. During snails and clamps growth, there were found several genus of pathogenic bacteria such as Salmonella, Vibrio, Aeromonas, Pseudomonas, Citrobacter, Proteus, Shigella, Hafnia, and Yersinia. The results showed that Pari island waters was suitable for developing shellfish aquaculture dog conch and blood clamps. Keywords: bacteria, parameter, shellfish, aquaculture.
MICROBIAL COMMUNITY DURING BIOREMEDIATION EXPERIMENTAL ON OIL SPILL IN COASTAL OF PARI ISLAND Lies Indah Sutiknowati
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 3 No. 1 (2011): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (592.574 KB) | DOI: 10.29244/jitkt.v3i1.7838

Abstract

There is an information how to identify hydrocarbon degrading bacteria for bioremediation of marine oil spill. We have Bioremediation treatment for degradation of oil spill on Pari island and need two kind of experiment there are tanks experiment (sampling 0 to 90 days) and semi enclosed system (sampling 0 to 150 days). Biostimulation with nutrients (N and P) was done to analyze biodegradation of hydrocarbon compounds. Experiment design using fertilizer Super IB and Linstar will stimulate bacteria can degrade oil, n-alkane, and alkane as poly aromatic hydrocarbon. The bacteria communities were monitored and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE) and Clone Library; oil chemistry was analyzed by Gas Chromatography Mass Spectrometry (GCMS). DNA (deoxyribonucleic acid) was extracted from colonies of bacteria and sequence determination of the 16S rDNA was amplified by primers U515f and U1492r. Strains had been sequence and had similarity about 90-99% to their closest taxa by homology Blast search and few of them suspected as new species. The results showed that fertilizers gave a significant effect on alkane, PAH and oil degradation in tanks experiment but not in the field test. Dominant of the specific bacteria on this experiment were Alcanivorax, Marinobacter and Prosthecochloris. Keywords: Bioremediation, Biostimulation, DGGE, PAH, Pari Island
SEAWATER QUALITY FOR SHRIMP MARICULTURE BASED ON MICROBIOLOGY PARAMETERS Lies Indah Sutiknowati
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 6 No. 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.604 KB) | DOI: 10.29244/jitkt.v6i1.8638

Abstract

Seawater quality plays an important factor for the success of mariculture such as microbiology paramater. The research aimed  to analize the waters quality for shrimp mariculture in Barru, Pangkep, and Maros based on bacteriology parameters.  The research were carried out in June 2012. Bacteriology parameters analysed were total coliform, E.coli, pathogen, heterotrophic, halotolerant, and phosphate-nitrate-ammonia bacteria. Coliform and E.coli bacteria were analysed based on filtration. Pathogenic bacteria was identified using biochemical test. Heterotrophic, halotolerant, and phosphate-nitrate-ammonia bacteria were analysed using pour plate. The results from shrimp aquaculture showed the total abundance of coliform cell of >1000 colony forming unit (cfu)/100 ml, E.coli of 0-4 cfu/100ml, heterotrophic bacteria of about (31-176)x103 cfu/ml, and halotolerant bacteria of about (31-375)x103 cfu/ml. The results from sediment for heterotrophic bacteria was about (350-3920)x103 cfu/ml, halotolerant of about (350-4980)3x10 cfu/ml, and phosphate-nitrate-ammonia bacteria of about 14-46 cfu/ml. The pathogen bacteria waere found such as genus Aeromonas, Pseudomonas, Proteus, Citrobacter, Shigella and Yersinia. The dominant pathogens in shrimp aquaculture water and sediment were Proteus and Citrobacter. The results indicated that seawaters in Barru, Pangkep, and Maros can be used for mariculture including shrimp Panaeid. Keywords: bacteria, parameter, marineculture, shrimp.
MIKROBA PENCEMAR DI PERAIRAN TELUK JAKARTA Nur Fitriah Afianti; Lies Indah Sutiknowati
OSEANA Vol 42 No 3 (2017)
Publisher : Pusat Penelitian Oseanografi LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oseana.2017.Vol.42No.3.83

Abstract

MICROBE CONTAMINANT IN JAKARTA BAY WATERS. High anthropogenic activity in Jakarta Bay is believed to contribute a fairly high contamination as evidenced by microbial contaminants such as Coliform and Escherichia coli. The high density of Coliform and E. coli as indicators of domestic contamination in Jakarta Bay as a result of an increase in the resident numbers who bring the flow of water to the springhead of the Jakarta Bay. It can be said that the polluted waters of Jakarta Bay and marine biota should be cultivated in this area as green shell should not consumed, because it can infect and cause gastrointestinal diseases. For that purpose, a research of microbial pollutants was conducted in Jakarta Bay in July 2015, and the results showed a very high density, especially in the West.
Co-Authors Nur Fitriah Afianti