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BOEY PENG LIM
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack LUTHFI AZIZ MAHMUD SIREGAR; CHAN LAI KENG; BOEY PENG LIM
HAYATI Journal of Biosciences Vol. 16 No. 2 (2009): June 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (525.15 KB) | DOI: 10.4308/hjb.16.2.69

Abstract

The effect of various macronutrients, micronutrients and sucrose on growth and canthinone alkaloid production in cell suspension cultures of Pasak Bumi (Eurycoma longifolia Jack) was investigated. The optimum macronutrients and micronutrients content for the high alkaloid production of E. longifolia Jack was different to that found in the Murashige and Skoog (MS) medium. The highest amount of alkaloids, 9-hydroxycanthin-6-one and 9-methoxycanthin-6-one, could be obtained from E. longifolia Jack cells cultured in modified MS liquid medium that containing macronutrients: 21.50 mM NH4NO3, 14.25 mM KNO3, 7.50 mM CaCl2•2H2O, 2.50 mM MgSO4•7H2O, 1.45 mM KH2PO4, while content of micronutrients was 0.233 mM FeNa-EDTA, 0.215 mM MnSO4•4H2O and without CuSO4•5H2O. Increased sucrose concentration to 4.00% (w/v) in modified MS liquid medium could increase total of two-alkaloid. The modification of macronutrients and micronutrients concentration based the optimum production of biomass was obtained MSBs medium that producing high biomass but also increasing the production of 9-hydroxycanthin-6-one. The modification of macronutrients or macronutrients and micronutrients based the optimum total of two-alkaloid was obtained MSC and MSD medium that producing low fresh weight but producing the high 9-hydroxycanthin-6-one. Key words: Pasak Bumi, 9-hydroxycanthin-6-one, 9-methoxycanthin-6-one, macronutrients, micronutrients, sucrose
PENGARUH KASEIN HIDROLISAT DAN INTENSITAS CAHAYA TERHADAP PRODUKSI BIOMASSA DAN ALKALOID CANTHINONE DI DALAM KULTUR SUSPENSI SEL PASAK BUMI (Eurycoma longifolia Jack) Siregar, Luthfi Aziz Mahmud; Keng, Chan Chan; Lim, Boey Peng
Makara Journal of Science Vol. 14, No. 1
Publisher : UI Scholars Hub

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Abstract

Effects of Casein Hydrolisate and Light Intensity on Production of Biomass and Canthinone Alkaloid in Cell Suspension Cultures of Pasak Bumi (Eurycoma longifolia Jack). A study on the effect of several concentrations of casein hydrolysate and light intensity to produce biomass and canthinone alkaloid in cell suspension culture of Pasak Bumi (Eurycoma longifolia Jack) was conducted. The research was an experimental study with block random design non factorial. For study of casein hydrolysate was used 8 (eight) of concentration treatments, i.e. 0.00, 0.10, 0.25, 0.50, 0.75, 1.00, 2.00, dan 5.00% (w/v). Beside for study of different light intensity effect was used five of light intensity, i.e. 0, 190, 290, 585, and 1525 lux. The results showed that addition 0.1–2.0% (w/v) of casein hydrolysate into culture medium did not show the effect on production of cell biomass compare with without casein hydrolysate. Total of alkaloid produce increased two fold in the culture medium containing 0.1% casein hydrolysate. The medium with addition 5% casein hydrolysate significantly decrease of increased fresh weight, dry weight and total of alkaloid compare with medium without or additions of casein hydrolysate at 0.1–2.0% concentration. Besides, the modifications on light intensity (0–1525 lux) did not effect the production of cell biomass in culture medium, but the place of E. longifolia Jack cell suspension culture at light of 1525 lux was found have to stimulate increased the total of 9- methoxycanthin-6-one in cells.
Co-Authors CHAN LAI KENG Keng, Chan Chan Luthfi Aziz Mahmud Siregar