A. Citraresmini
Center for Application of Isotopes and Radiation Technology, National Nuclear Energy Agency Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia

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The use of 32P Method to Evaluate the Growth of Lowland Rice Cultivated in a System of Rice Intensification (SRI) A. Citraresmini; I. Anas; Nurmayulis Nurmayulis
Atom Indonesia Vol 39, No 2 (2013): August 2013
Publisher : PPIKSN-BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.311 KB) | DOI: 10.17146/aij.2013.236

Abstract

A pot experiment has been conducted to evaluate the growth of the Dyah Suci, a lowland rice variety, in an SRI (System of Rice Intensification) planting system. The phosphorus-32 (32P) isotope technique was used to evaluate the growth of plants in relation with their phosphorus uptake. The uptake was assumed to vary in the same direction as the growth of the plant. The 32P uptake is assumed to vary in the opposite direction to the plant’s total phosphorus uptake. Here the 32P uptake is expressed in count per minutes (cpm) which is then transformed to disintegration per minute (dpm). The results show that, in terms of promoting the plant’s uptake of phosphorus, the SRI planting system is superior to the conventional planting system, and it is manifested in the higher dry weight of straw and grain. From this experiment it is concluded that the 32P method can be used satisfactorily as a tool for explaining therelation between P-uptake and plant growth.Received: 04 Juny 2013; Revised: 22 August 2013; Accepted: 30 August 2013
Measuring the BNF of Soybean Using 15N-Labelled Urea with Different Atom Excess (A.E.) Content A. Citraresmini; H. Halim; E.L. Sisworo
Atom Indonesia Vol 38, No 3 (2012): December 2012
Publisher : PPIKSN-BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/aij.2012.178

Abstract

The soybean is a legume which has an ability to supply its major nitrogen need by the biological nitrogen fixation (BNF) process. This process is made possible by nodules formed in their roots, colonized by Rhizobium sp.bacteria. An accurate estimation of N gained by BNF is necessary to predict the increase or decrease of chemical fertilizer-N requirements to increase soybean production. Among several methods, the 15N method was used to estimate the ability of legumes to perform BNF. The study involved soybean var. Willis (W) and a completely non-BNF soybean var. CV, which is termed as a standard crop. The standard crop is non-nodulated soybean, but it has the same main physiological traits with var. Willis. The aim of this study was to determine whether15N-labelled fertilizer with different %a.e. given to nodulated and non-nodulated soybean would not be of significant consequences for the calculation of N-BNF of W. The treatments applied were different rates of urea (20 kg N/ha and 100 kg N/ha) combined with different atom excess percentages (%a.e.)15N (2% and 10%). Thus, the combination of treatments were as follows:(1) W-ll (20 kg N; 2% a.e); (2) CV-hl (100 kg N; 2% a.e); (3) W-lh (20 kg N; 10% a.e); (4) CV-hh (100 kg N; 10% a.e); (5) CV-ll (20 kg N; 2% a.e); (6) W-hl (100 kg N; 2% a.e); (7) CV-lh (20 kg N; 10% a.e); (8) W-hh (100 kg N; 10% a.e). The result of the experiment showed that a high %a.e. with a low rate of 15N and a low %a.e. with a high rate of N should be used to study the %N-BNF of nodulated plants.Received: 16 March 2012; Revised: 14 December 2012; Accepted: 17 December 2012