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Senny J. Bunga
Jurusan Tanaman Pangan dan Holtikultura Politeknik Pertanian Negeri Kupang
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Pengaruh Kerapatan Populasi dan Dosis SP-36 Pada Tanaman Kacang Merah Varietas Inerie di Dataran Rendah Terhadap Kualitas Fisiologis dan Kimiawi Benih Yosefina Lewar; Yohanis H. Dimu Heo; Senny J. Bunga
Partner Vol 22, No 1 (2017): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v22i1.236

Abstract

A good quality seed is obtained from good and appropriate production process. The use of good quality seeds could increase crop production plants, including Inerie variety of red bean. This plant could be grown in the lowlands having modified micro-climate. One simple technology to modify the micro-climate of the plant is through plant population density setting followed by the use of balanced fertilizer, including P fertilizer which could result in high quality seeds.  This research was conducted in the Practical Garden of Kupang State Agricultural Polytechnic from April to November 2015. The results of the study  showed that 1) the population density affected the levels of seed free fatty acids in the lowest population density of 36 plants / 3 m2 (50 cm x 30 cm);  2) the doses of SP-36 fertilizer affected the germination percentage of the seeds at the highest dose of 200 kg / ha SP-36, and free fatty acid of the seed on lowest level without SP-36 fertilizer , and;  3) there was interaction between the treatment plant and the population density of the dose SP- 36 fertilization. Treatment population density of 36 plants (spacing of 50 cm x 20 cm) with a dose of SP-36 200 kg / ha was the best effect on the rate of growth of seeds, simultaneity sprouted seeds and the content of P-Total on Inerie variety of red beans planted in lowlands eventhough did not have a different result with a spacing of 40 x 20 cm with a dose of SP-36 200 kg / ha. The results of the study served as a model in the development of Inerie variety of red bean in lowlands. Keywords: Production, Quality, Seed, Inerie Red Beans, Lowlands
Δ6 FATTY ACYL DESATURASE GENE IN A SOUTHERN BLUEFIN TUNA (Thunnus maccoyii) CELL LINE Senni Juniawati Bunga; Kathy Schuller
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 2 (2015): June 2015
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.923 KB) | DOI: 10.23869/107

Abstract

Fish is the main source of ω-3 long chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5ω-3) and docosahexaenoic acid (DHA, 22:5ω-3), which have positive effects on human health and can be beneficial in human diet context. Studies involving fatty acyl desaturase (Fads) and elongase of very long chain fatty acids (Elovl) enzymes that convert C18 PUFA to C20/22 LC-PUFA have been performed in some fish species. However, very little is known about LC-PUFA biosynthesis in tuna species. This study investigated the Δ6 Fads gene performance in the SBT cell line. The Δ6 Fads nucleotide sequences from various fish species were identified and retrieved to compare them with the SBT Δ6 Fads nucleotide sequence. The Δ6 Fads gene was performed using real time PCR (RT-PCR) and then was compared it with β-actin gene performance as a reference housekeeping gene. By performing multiple sequence alignments and comparing the highly conserved regions among fish Δ6 Fads sequences, the SBT Δ6 Fads nucleotide sequence was determined to be ≥ 75% identical to the other fish Δ6 Fads sequences. The results showed that when the SBT Δ6 Fads and β-actin cDNAs were performed in a standard PCR system and the products were analysed by electrophoresing them on a 2% (w/v) agarose gel, the target genes that were obtained were similar to the expected sizes. The observed band size for the Δ6 Fads PCR product was 207 bp and for the β-actin PCR product was 98 bp. The presence of the observed bands indicated that the primer pairs that were designed and used were successfully amplified the target genes. The results of this study might provide relevant information to support further investigating of the desaturase and elongase gene expression that might contribute to a better understanding of ω-3 LC-PUFA biosynthesis in fish.
Co-Authors Kathy Schuller Yohanis H. Dimu Heo Yosefina Lewar, Yosefina