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All Journal Jurnal Dampak International Conference on Coastal and Delta Areas Jurnal Purifikasi Serambi Engineering
Harmin Sulistyaning Titah
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Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Energy Engineering Environmental Science Industrial & Manufacturing Engineering Other

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Journal : International Conference on Coastal and Delta Areas

 1 
Identification of Diesel Resistant Bacteria that Isolated from Ship Dismantling Area in Madura Coastal Harmin Sulistyaning Titah; Herman Pratikno; Atiek Moesriati; Rizky Islami Putera; Muhammad Fauzul Imron
International Conference on Coastal and Delta Areas Vol 3 (2017): The 3rd International Conference on Coastal and Delta Areas
Publisher : International Conference on Coastal and Delta Areas

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Ship dismantling activity is one industry that caused diesel contamination in environment. One of technology that can be used to remediate diesel contaminated area is bioremediation. Bioremediation can be conducted using bioaugmentation technique. The objective of this study was to identifity of diesel resistant bacteria using biochemicals test methods. The seawater and coastal soil samples were collected from area study using a sterilized bottles. All samples were shaker at 150 rpm for 1 h, after that samples were taken and serially diluted from 10-1 to 10-7. The diluted sample was inoculated on nutrient agar plates by pour plate method. The plate was incubated for about 24-48 hours and the growth of microorganisms was noted. Bacteria with difference of colony morphologies were selected. The cell morphology was determined microscopically after Gram-stain preparation. The isolates were identified using Microbact GNB 12A and 12B (Oxoid, UK) identification kit. This is a miniaturized computer aided identification system for the identification of organisms belonging to the genus Acinetobacter and Vibrio. Keywords: bacteria, bioremediation, coastal, seawater, ship dismantling
The ability of Avicennia marina (Api-api putih) to Uptake Heavy Metal of Chromium at Wonorejo Coastal in Surabaya Herman Pratikno; Harmin Sulistyaning Titah; Handayanu Handayanu; Bintang Respati Dwi Harnani
International Conference on Coastal and Delta Areas Vol 3 (2017): The 3rd International Conference on Coastal and Delta Areas
Publisher : International Conference on Coastal and Delta Areas

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The increasing of industries and population growth are the main sources of contamination in the river. Wonorejo river is one of rivers in Surabaya that flow to east coast Surabaya. Concentration of Chromium (Cr) at estuary of Wonorejo River was 0.0325 mg/L and it was 2.7761 mg/L in sediment in 2007. Meanwhile, Cr at east coast of Wonorejo was 0.418 μg/L, it was indicating that upper than standart for biota in sea, 0.005 μg/L (KepMenLH No. 51 tahun 2004). In this research will be measured concentration of Cr from sediment and roots of Avicennia marina at Wonorejo River estuary. And then, it was calculated Bio-consentration Factor (BCF) value of A. marina. Transect method was used to determine the sampling point of mangrove root A. marina and sediment at estuary of Wonorejo River. Mangrove and sediment roots were analyzed using the Atomic Absorption Spectrophotometer (AAS) method, previously samples of roots and sediments were extracted using the wet destruction method. Parameters supporting in this research were measured include temperature using thermometer, salinity using salinometer and pH using pH meter for sediment. Based on the results, the average concentration of Cr in the sediment in transect 1 were 47 mg/kg until to 66.5 mg/kg. The concentration of Cr in roots of A. marina were 28 mg/kg until to 92.25 mg/kg. The BCF value in A. marina were 0.89 to 1.35. Based on the BCF value, it indicated that A. marina was a hyperacumulator species for heavy metals of Cr. Keywords: Avicennia marina, BCF, coast, chromium, root, sediment
Co-Authors , Srifatunningsih Atiek Moesriati Bintang Respati Dwi Harnani Handayanu Handayanu Herman Pratikno, Herman Jihan Luthfiyah, Al Fatih Fadhilah Muhammad Fauzul Imron Rizky Islami Putera