Claim Missing Document
Check
Articles

Found 9 Documents
Search

Identification of Diesel Resistant Bacteria that Isolated from Ship Dismantling Area in Madura Coastal Harmin Sulistyaning Titah; Herman Pratikno; Atiek Moesriati; Rizky Islami Putera; Muhammad Fauzul Imron
International Conference on Coastal and Delta Areas Vol 3 (2017): The 3rd International Conference on Coastal and Delta Areas
Publisher : International Conference on Coastal and Delta Areas

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Ship dismantling activity is one industry that caused diesel contamination in environment. One of technology that can be used to remediate diesel contaminated area is bioremediation. Bioremediation can be conducted using bioaugmentation technique. The objective of this study was to identifity of diesel resistant bacteria using biochemicals test methods. The seawater and coastal soil samples were collected from area study using a sterilized bottles. All samples were shaker at 150 rpm for 1 h, after that samples were taken and serially diluted from 10-1 to 10-7. The diluted sample was inoculated on nutrient agar plates by pour plate method. The plate was incubated for about 24-48 hours and the growth of microorganisms was noted. Bacteria with difference of colony morphologies were selected. The cell morphology was determined microscopically after Gram-stain preparation. The isolates were identified using Microbact GNB 12A and 12B (Oxoid, UK) identification kit. This is a miniaturized computer aided identification system for the identification of organisms belonging to the genus Acinetobacter and Vibrio. Keywords: bacteria, bioremediation, coastal, seawater, ship dismantling
The ability of Avicennia marina (Api-api putih) to Uptake Heavy Metal of Chromium at Wonorejo Coastal in Surabaya Herman Pratikno; Harmin Sulistyaning Titah; Handayanu Handayanu; Bintang Respati Dwi Harnani
International Conference on Coastal and Delta Areas Vol 3 (2017): The 3rd International Conference on Coastal and Delta Areas
Publisher : International Conference on Coastal and Delta Areas

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The increasing of industries and population growth are the main sources of contamination in the river. Wonorejo river is one of rivers in Surabaya that flow to east coast Surabaya. Concentration of Chromium (Cr) at estuary of Wonorejo River was 0.0325 mg/L and it was 2.7761 mg/L in sediment in 2007. Meanwhile, Cr at east coast of Wonorejo was 0.418 μg/L, it was indicating that upper than standart for biota in sea, 0.005 μg/L (KepMenLH No. 51 tahun 2004). In this research will be measured concentration of Cr from sediment and roots of Avicennia marina at Wonorejo River estuary. And then, it was calculated Bio-consentration Factor (BCF) value of A. marina. Transect method was used to determine the sampling point of mangrove root A. marina and sediment at estuary of Wonorejo River. Mangrove and sediment roots were analyzed using the Atomic Absorption Spectrophotometer (AAS) method, previously samples of roots and sediments were extracted using the wet destruction method. Parameters supporting in this research were measured include temperature using thermometer, salinity using salinometer and pH using pH meter for sediment. Based on the results, the average concentration of Cr in the sediment in transect 1 were 47 mg/kg until to 66.5 mg/kg. The concentration of Cr in roots of A. marina were 28 mg/kg until to 92.25 mg/kg. The BCF value in A. marina were 0.89 to 1.35. Based on the BCF value, it indicated that A. marina was a hyperacumulator species for heavy metals of Cr. Keywords: Avicennia marina, BCF, coast, chromium, root, sediment
PENGARUH PENGGUNAAN SERBUK BIJI KELOR (MORINGA OLEIFERA) TERHADAP PENURUNAN KONSENTRASI CU(II) DALAM LIMBAH CAIR BUATAN DENGAN PROSES ADSORPSI harmin sulistyaning titah
Purifikasi Vol 8 No 1 (2007): Jurnal Purifikasi
Publisher : Department of Environmental Engineering-Faculty of Civil, Environmental and Geo Engineering. Institut Teknologi Sepuluh Nopember, Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25983806.v8.i1.109

Abstract

Kelor (Moringa oleifera) is a kind of bush plant, which has a number of application potential, started from the leaf till the root. The seeds can produce 4-(alpha-L-rhamnosyloxy)benzyl isothiocyanate compound which can bind Cu(II). Besides, the seed also contains protein with cysteine and methionine components, which can bind Cu(II) from S-H group. In this research, copper adsorption by horse radish seed was measured, using complete powdered seeds (with epidermis skin part) and incomplete seeds (without epidermis). Two sizes of the powdered seed of 35/80 and 80/115 mesh sizes were applied. The initial concentrations of Cu(II) were 0; 0,25; 1; 5; 10 mg/L. The Cu(II) concentrations were analyzed using Atomic Absorption Spectrophotometer. In the batch process, the removal efficiency of Cu(II) was up to 97,07 % at initial concentration of 10 mg/L, using the incomplete seeds of 80/115 mesh size. In a continuous process with a flowrate of 0,5065 ml/minute Cu(II) removal was up to 96,2 %, and the adsorption capacity was 0,000149 mg adsorbate/mg adsorbant.
KAJIAN KARAKTERISTIK HASIL PENGGALIAN TIMBUNAN SAMPAH DI TPA KOTA KEDIRI SEBAGAI KOMPOS harmin sulistyaning titah
Purifikasi Vol 8 No 2 (2007): Jurnal Purifikasi
Publisher : Department of Environmental Engineering-Faculty of Civil, Environmental and Geo Engineering. Institut Teknologi Sepuluh Nopember, Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25983806.v8.i2.126

Abstract

The municipal solid waste (MSW) generation rate in Kediri was 697,768 m3 per day. The operation period of Kediri’s final disposal site (FDS) is 3-4 years. This condition provides an idea to excavate the FDS as an effort of rehabilitation, and to evaluate the fine material quality as compost. Four sampling sites were selected in the study area: site I (1 m, 3 years old), site II (2 m, 3 years old), site III (1 m, 1 year old), and site IV (2 m, 1 year old). Each sample was separated, air dried, and sieved. The highest fine material component (66,5%) was found in sampling site II. The colour of this fine material was brownish black and the odor was like soil. Characteristics of compost from all sampling sites met the compost quality standard according to SNI 19-7030-2004. Some parameters; however, exceeded the standards. These parameters werer volatile solid (> 80%), carbon (45-47%), C/N ratio (33 – 40), and faecal coli (>1000 MPN/gr). Therefore, the fine material from Kediri’s FDS could not be used as compost. The compost quality was best in FDS age of more than 3 year old and excavated from a depth of more than 2 m.
PENURUNAN WARNA LIMBAH CAIR INDUSTRI PENCELUPAN TEKSTIL DENGAN MENGGUNAKAN ADSORBEN ARANG SEKAM PADI harmin sulistyaning titah
Purifikasi Vol 7 No 1 (2006): Jurnal Purifikasi
Publisher : Department of Environmental Engineering-Faculty of Civil, Environmental and Geo Engineering. Institut Teknologi Sepuluh Nopember, Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25983806.v7.i1.236

Abstract

Salah satu bentuk proses pengolahan air limbah secara fisik-kimia adalah adsorpsi. Pada pada proses adsorpsi digunakan adsorben untuk menyerap adsorbat. Sekam padi sebagai hasil sampingan pengolahan padi belum termanfaatkan dengan baik. Sekam padi dengan kandungan karbon dan SiO2 yang tinggi merupakan bahan yang baik untuk menghasilkan arang, Pemanfaatan sekam padi sebagai adsorben dengan menggunakan NaOH sebagai bahan aktifator untuk meningkatkan kapasitas adsorpsi dari arang yang dihasilkan. Untuk mengetahui kemampuan dari sekam padi dilakukan uji secara batch dengan variasi konsentrasi adsorben dan kecepatan agitasi dalam menurunkan warna limbah pencelupan tekstil. Hasil penelitian menunjukkan bahwa efisiensi penurunan warna tertinggi dengan adsorben sekam padi pada konsentrasi 100 mg/l sebesar 23 %.
UJI TOKSISITAS AKUT LIMBAH PABRIK SARUNG TENUN PADA IKAN MUJAIR (Tilapia mossambicus) harmin sulistyaning titah
Purifikasi Vol 6 No 2 (2005): Jurnal Purifikasi
Publisher : Department of Environmental Engineering-Faculty of Civil, Environmental and Geo Engineering. Institut Teknologi Sepuluh Nopember, Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25983806.v6.i2.282

Abstract

Limbah cair pabrik sarung tenun mengandung zat warna dengan kandungan COD, TSS yang tinggi dan logam berat Chrom (Cr) yang langsung dibuang ke sungai akan menimbulkan pencemaran. Dengan adanya uji toksisitas dapat diketahui nilai toksisitas limbah cair tersebut melalui prosentase kematian ikan Mujair (Tilapia mossambicus) sebagai salah satu biota uji. Metode penelitiannya adalah dengan prinsip acute toxicity test yang didahului dengan range finding test sehingga dapat ditemukan letal concentration-50 populasi biota uji, dimana nilai ini merupakan konsentrasi limbah yang bersifat toksik. Dilakukan pula uji untuk mengetahui kandungan Cr yang terdapat dalam tubuh biota uji dengan metode Ashing Furnice dan kerusakan pada insang dengan metode hispatologi insang. Berdasarkan penelitian yang dilakukan, LC-50, 96 jam pada ikan Mujair adalah 0,945%±0,045% dengan kandungan rata-rata COD 23,86 mg/l, TSS 33,28 mg/l, logam berat Cr total 0,06 mg/l dan zat warna 19,74 mg/l. Zat warna pada ikan Mujair tersebut mengandung senyawa azo = 1,55 mg/l, senyawa quinon = 1,44 mg/l dan senyawa naftol = 3,36 mg/l. Adanya kematian biota uji disebabkan adanya zat warna yang mengandung senyawa azo yang bersifat toksik. Adanya limbah pabrik sarung tersebut juga mengakibatkan perubahan histopatologi insang berupa hyperplasia lamella.
STUDI PENURUNAN NITROGEN AMONIUM LIMBAH TAMBAK UDANG INTENSIF DENGAN MENGGUNAKAN ROUGHING BIOFILTER HORISONTAL harmin sulistyaning titah
Purifikasi Vol 5 No 1 (2004): Jurnal Purifikasi
Publisher : Department of Environmental Engineering-Faculty of Civil, Environmental and Geo Engineering. Institut Teknologi Sepuluh Nopember, Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (88.35 KB) | DOI: 10.12962/j25983806.v5.i1.302

Abstract

The decrease in nitrogen concentration during the nitrification process of wastewater from the intensive shrimp ponds was observed by using the Horizontal Roughing Biofilter. The Horizontal Roughing Biofilter was run continuously with the low loadings and detention time variation of 1.0 , 1.5, 2.0 and 2.5 days. The waste water was taken from the effluent of shrimp ponds in Sidoarjo. The experiment showed that the average removal of nitrogen concentration was greater than 90%. The nitrification process followed the zero order reaction, and the reaction rate were influenced by the initial concentration of nitrogen and the detention time.
Fikoremediasi Pb Dengan Variasi C:N Oleh Mikroalga Dunaliella sp. Jihan Luthfiyah, Al Fatih Fadhilah; Harmin Sulistyaning Titah
Dampak Vol. 22 No. 2 (2025)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/dampak.22.2.104-112.2025

Abstract

Pada era global industri ini ditemukan air tercemar bersumber dari aktivitas pertanian,peternakan,industri,perhotelan yang memiliki nutrien tinggi dan kandungan logam berat Pb mencapai 1 mg/L. Hal ini akan berdampak pada makhluk hidup seperti gangguan saraf, gangguan fisiologis dan kematian. Penerapan teknologi remediasi oleh mikroalga sudah diterapkan dalam penyisihan Pb. Akan tetapi perlu dipertimbangkan keseimbangan rasio nutrisi C:N yang terkandung pada media air tersebut. Perbandingan nutrisi karbon dan nitrogen digunakan mikroalga dalam kebutuhan sumber energi dan sintetis protein. Penggunaan mikroalga hijau Dunaliella sp. adalah solusi yang dipilih dalam penerapan remediasi air tercemar Pb sebesar 76-80%. Uji rasio C:N sangat penting untuk memastikan bahwa kondisi pertumbuhan mikroorganisme optimal dan nutrisi terpenuhi. Variasi rasio C:N yang digunakan penyisihan Pb 1 mg/L adalah 100:7,100:13,100:32 dengan variasi intensitas cahaya 2000,4000,6000 lux. Variabel respon dari percobaan ini adalah penurunan kadar Pb,kepadatan sel mikroalga, pH, suhu,CO2, salinitas, dissolved oxygen, dan konsentrasi bikarbonat diamati selama 14 hari. Sebelum dilakukan percobaan 14 hari dilakukan propagasi. Kepadatan sel menggunakan hemositometer dan konsentrasi Pb menggunakan Spektrofotometer Serapan Atom. Hasil dari pengujian variasi rasio nutrisi C:N didapatkan rasio C:N seimbang dan optimal dalam penyisihan Pb pada mikroalga Dunaliella sp. 77% pada rasio C:N 100:32 intensitas cahaya 6000 lux. Dunaliella sp. menghasilkan kepadatan sel pada rasio tersebut mencapai 535,43 x10.000 sel/mL dengan laju pertumbuhan rata-rata 4,80 sel/mL/hari pada parameter kondisi lingkungan mendukung. Kata Kunci: Dunaliella sp.,Fikoremediasi,Pb, Rasio C:N
Daya Serap CO2 Tanaman Pengisi Ruang Terbuka Hijau Pada Zona Riparian di Kali Surabaya , Srifatunningsih; Harmin Sulistyaning Titah
Jurnal Serambi Engineering Vol. 10 No. 3 (2025): Juli 2025
Publisher : Faculty of Engineering, Universitas Serambi Mekkah

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Climate change is a phenomenon caused by human activities that increase greenhouse gas emissions, particularly carbon dioxide (CO₂), thereby accelerating global warming. In Surabaya City, the Surabaya River serves as a primary raw water source for the local water utility (PDAM). However, land-use changes in the riparian zone—from Green Open Spaces (RTH) to residential and industrial areas—have reduced environmental carrying and buffering capacities due to rising CO₂ emissions. This study aims to analyze the CO₂ absorption capacity of RTH in the riparian zone of the Surabaya River, specifically in the Sepanjang – Gunungsari segment. The methods used include literature review, collection of primary and secondary data, and analysis of RTH suitability based on applicable regulations. The calculation of CO₂ absorption capacity considered the area of existing RTH and the types of vegetation present. The results show that the existing RTH area is only 63,000 m², significantly below the ideal requirement of 124,857 m². With the current vegetation composition, the total CO₂ absorption capacity is estimated at 594,628.69 kg CO₂ per year. These findings highlight the importance of maintaining and expanding RTH in riparian areas to support climate change mitigation in urban environments.