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Sublethal toxicity test of mercury (Hg) in the flesh and tissue of Tilapia (Oreochromis niloticus) Yusnaini Yusnaini; Emiyarti Emiyarti; Indriyani Nur; Oce Astuti; Rahmad Sofyan Patadjai
Journal Omni-Akuatika Vol 16, No 2 (2020): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2020.16.2.749

Abstract

This study tested the sublethal toxicity of mercury (Hg) in the flesh and tissue of tilapia (Oreochromis niloticus). This research used an experimental method completely randomized design (CRD) with 3 treatments and 3 replications. The treatments used different Hg concentrations: Treatment A = 0.08 ppm; B = 0.16 ppm; C = 0.24 ppm; and the control group = without the addition of Hg. The parameters observed were the accumulation of mercury (Hg) in the flesh and tissue damage (histopathology) of the gills, kidneys and liver of tilapia. The results showed that the highest Hg accumulation was at the highest Hg concentration (treatment C = 0.24 ppm). Tissue damage was mainly found in the kidneys, liver and gills of fish exposed to Hg with indications of bleeding, tubular necrosis, vacuolization of epithelial cells, and mononuclear cell infiltration. The results showed that the toxicity of mercury to the organs increased with the increase in the concentration of Hg in water. Keywords : flesh, mercury, Oreochromis niloticus, sublethal, tissue
Production and Application of Organic Fertilizer for White Leg Shrimp and Milkfish Cultivation Indriyani Nur; Yusnaini Yusnaini; Baheri Baheri
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 7, No 4 (2021): December
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1149.383 KB) | DOI: 10.22146/jpkm.55842

Abstract

The slow growth of white leg shrimp and milkfish cultivation became a concern in the location of community service activity (PKM). This was mainly due to food limitation, especially concerning the difficulty in natural food development. The purpose of this endeavor is to apply the production technology and application of organic fertilizer for the cultivation of white leg shrimp and milkfish along the marginal embankments. This endeavor was carried out in April to November 2019 in Mondoe Village, Konawe Selatan District, Southeast Sulawesi. This activity was manifested by providing production facilities, training, production of organic fertilizer, the use of organic fertilizer, white leg shrimp and milkfish cultivation, monitoring, and activities evaluation. The implementation of this activity was able to increase the knowledge, perception, and skills of participants as partners in producing fermented organic fertilizer. The application of the use of organic fertilizer was able to provide alternatives and overcome the limitation of fertilizer for aquaculture businesses in embankments. The results of this PKM were proven to be able to improve partners' knowledge and skills and provide solutions in overcoming the limitation of fertilizer especially in terms of aquaculture businesses in embankments.
PENERAPAN KANTONG JARING UKURAN MINI UNTUK PRODUKSI LOBSTER UKURAN SUPER DI DESA TAPULAGA KECAMATAN SOROPIA KABUPATEN KONAWE PROVINSI SULAWESI TENGGARA Yusnaini; Muhammad Ramli; Indriyani Nur; Muhammad Idris; Agus Kurnia; Irdam Riani
Panrita Abdi - Jurnal Pengabdian pada Masyarakat Vol. 5 No. 3 (2021): Jurnal Panrita Abdi - Juli 2021
Publisher : LP2M Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/pa.v5i3.11630

Abstract

Abstract. Lobster is a fishery resource of significant economic importance. The supply of lobster is still mainly derived from natural capture. In addition, to the seed problem, lobster is cannibal and growth is relatively slow in cultivation. The solution applied is a cultivation system using floating net bags (KJA) of small size (dimensions of length and width of 1 m and height of 1.5 m). The aims of this activity is to increase the knowledge and skills of the technical guidance participants, especially the lobster farming group. It also provides production facilities in the form of small zise of cage, juvenile lobster and feed for the production of super sized lobsters. The results of this activity were increased knowledge 69-100% and skills 44-100%  of lobster culture partners in the mini KJA system. As a result, the weight of the pearl lobster (Panulirus ornatus) had an average increase 38 g per month, while the mortality rate was < 10 %. Selective harvesting was carried out with juvenile measuring an average of 200 g/lobster which could reach a weight of >500 g after being cultivated for 6-10 months. The such size of the lobster is super. The advantage of cultivation is to increase the weight (biomass) and price categories. The juvenile prices were Rp 350,000/kg or Rp 80,000/lobster, after reaching the super size the price increased to Rp 900,000/kg or Rp 450,000/lobster. The cultivation system using mini size net bags can be an alternative to the developmen of lobster culture.               Abstrak. Lobster sebagai komoditas perikanan mempunyai nilai ekonomis penting. Pasokan lobster masih dominan berasal dari penangkapan di alam. Selain masalah benih, kendala lain budidaya adalah lobster bersifat kanibal dan pertumbuhan relatif lambat. Solusi yang diterapkan adalah sistem budidaya dengan mengunakan kantong jaring apung (KJA) berukuran mini/kecil (dimensi panjang dan lebar 1 m serta tinggi 1,5 m). Tujuan kegiatan  adalah meningkatkan pengetahuan dan keterampilan peserta bimbingan teknis, khususnya kepada kelompok yang membudidayakan lobster. Dilakukan juga menyediakan sarana produksi berupa KJA mini, juvenil lobster dan pakan untuk produksi lobster berukuran super. Hasil kegiatan ini telah meningkatkan pengetahuan  69-100% dan keterampilan 44-100% mitra tentang budidaya lobster sistem KJA mini. Bobot lobster mutiara (Panulirus ornatus) yang dipelihara mengalami peningkatan bobot rata-rata 38 g/ekor/bulan dan  tingkat  kematian  <20%.  Panen selektif dilakukan, juvenil lobster berukuran rata-rata 200 g/ekor dapat mencapai bobot >500 g dan berkategori ukuran super, setelah dipelihara 6-10 bulan. Budidaya pembesaran meningkatkan bobot (biomassa) lobster dan kategori harga. Lobster mutiara berukuran 200-300 g, harganya sekitar  Rp 350.000/kg (Rp 80.000/ekor) dan setelah mencapai ukuran super harganya sekitar Rp 900.000/kg. (Rp 450.000/ekor yang berukuran 500 g/ekor). Sistem budidaya menggunakan kantong jaring ukuran mini dapat menjadi alternatif pada pengembangan budidaya lobster.
THE POTENTIAL OF EXTRACT OF LEAVES AND FLOWERS OF Lantana camara Linn. AS AN ANTIBACTERIAL FOR CATFISH (Clarias gariepinus) INFECTED BY Aeromonas hydrophila Indriyani Nur; Afiyfah Fitriani; Asnani Asnani
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (36.924 KB) | DOI: 10.15578/iaj.4.1.2009.41-45

Abstract

Freshwater catfish culture has been hampered by bacterial diseases. One of the agents of the bacterial disease is Motile Aeromonas Septicemia (MAS). The application of synthetic antibiotics has had some disadvantages such as bacterial resistance and undegradable in water. One of the potential antibacterial herbs is Lantana camara. Information of Lantana as an antibacterial on catfish is still limited. Therefore, the experiment of utilization of Lantana as an antibacterial for catfish should be conducted. The experiment was carried out to evaluate the potential of Lantana extract as an antibacterial of A. hydrophila for catfish. The completely randomized design was applied consisting of four treatments using two parts of the plant, leaves and flowers. The treatments were: A = 1,000 ppm of leaves; B = 2,000 ppm of leaves; C = 1,000 ppm of flowers; D = 2,000 ppm of flowers), and control. Lantana extracts were diluted into each culture media which had been infected with A. hydrophila. Several factors were observed in this experiment such as prevalence with of MAS disease, survival rate, percentage of haematocrites and total of leukocytes of fish blood. The results showed that the fish treated with 2,000 ppm of flowers extract had a lower in prevalence of MAS disease and higher in survival rate than those treated with 1,000 ppm; 2,000 ppm of leaves; and 1,000 ppm of flowers, respectively. However, percentage of haematocrytes and total of leucocytes was not influenced by the extracts from different parts of Lantana plant. In conclusion, 2,000 ppm of Lantana flowers extract might be useful as an antibacterial of A. hydrophila for catfish culture.
The Effect of Eyestalk Ablation on Several Immunologic Variables in Litopenaeus vannamei Indriyani Nur; Hartina Iyen; Yusnaini Yusnaini
Journal of Aquaculture and Fish Health Vol. 10 No. 1 (2021): JAFH Vol 10 No. 1 February 2021
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v10i1.18926

Abstract

Eyestalk ablation inflicted upon crustacean is generally performed in captivity to induce ovarian maturation. However, ablation is a hurtful action that may impose physiological stress and even affects survival. The immunologic repercussions of the ablation have not been well understood and thus this study aims to analyze the effect of ablation on immunologic variables through comparing the ablated Litopenaeus vannamei with those of unablated shrimps (control).  Unilateral left eyestalk ablation was inflicted on shrimp samples. Shrimp immune responses were observed based on the Total Haemocyte Count (THC), Differential Haemocyte Count (DHC) composing of hyaline cell (HC) counts and granular cell (GC) counts, glucose and uric acid levels, along with the weight gains of shrimps. Observed parametres were taken twice from 10 shrimps for each of the observed and control samples (i.e., on 6 h and 5 d elapsed time). The results show that the ablated shrimps group, after 6 h elapsed time, experienced significant decrease in THC (3.12 ± 0.75 x 106 cells ml-1), but recovered after 5 days (3.89 ± 0.96 x 106 cells ml-1) and were not significantly different from the control (5.16 ± 2.20 x 106 cells ml-1). Meanwhile, glucose level show significant decrease over 6 h and 5 d post ablation (108 and 93 mg dL-1, respectively) compared to the resulting responses from the control (35-36 mg dL-1). Differences in DHC for all cell types as well as uric acid in hemolymph were not significant between the observed and the control samples. Moreover, the ablated treatment did not affect the growth of vannamei after 5 d post ablation. The results revealed that changes occur on the physiological and immune parameters of shrimps due to the eye ablation treatment as shown by the decrease in the THC and glucose levels, especially within the early moments post treatment. However, at the end of the observation (5 d post ablation), shrimps exhibit signs of recovery from stress indicated by an increase in THC close to normal.