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All Journal Journal of Tropical Biodiversity and Biotechnology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Agus Hery Susanto
Faculty of Biology Jenderal Soedirman University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Intergeneric Hybridization between Phalaenopsis 2166 and Vanda ‘saint valentine’: Characterization of Parents Using ndhE cpDNA Partial Sequence Murni Dwiati; Agus Hery Susanto
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 3 (2021): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.65658

Abstract

An intergeneric cross between Phalaenopsis 2166 and Vanda ‘saint valentine’ has successfully produced protocorms that will be grown further to form seedlings. The present study aims to genetically characterize both parents by using ndhE partial gene as its sequence is shown polymorphic among five orchid genera of the subtribe Oncidiinae. The results reveal that the ndhE partial sequences of Phalaenopsis 2166 and Vanda ‘saint valentine’ are considerably homologous with those of Oncidium. However, alignment of ndhE partial sequences between both parents shows only 58% similarity, leading to the conclusion that a relatively high genetic difference between them may occur. 
Molecular Analysis of Synedrela Nodiflora (L.) Gaertn. Resistance Against Fomesafen using RAPD Markers Murni Dwiati; Agus Hery Susanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.290

Abstract

Both morphological and protein studies reveal that differences are observed between resistant Synedrella nodiflora against fomesafen and its susceptible wildtype. These are, however, more or less influenced by environmental factors, so that molecular analysis employing DNA markers is necessarily required. The methods involved total genomic DNA extraction using modified CTAB protocol following Doyle & Doyle (1990), RAPD marker amplification and visualization of RAPD markers prior to data analysis. Ten RAPD markers were used, but only seven of them showed polymorphism.  Calculation of genetic distance and variation was carried out employing PopGen software.Based on the RAPD markers used in this study, it can be concluded that genetic distance between susceptible and resistant S. nodiflora is higher than that within susceptible samples supporting our previous morphological and protein data, although genetic variation among susceptible individuals seems to be significantly high.
Co-Authors Murni Dwiati