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All Journal Biogenesis: Jurnal Ilmiah Biologi Prosiding Journal of Tropical Biodiversity and Biotechnology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Floribunda
Murni Dwiati
Faculty Of Biology Jenderal Soedirman University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology

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KARAKTERISASI RESISTENSI GULMA Synedrella nodiflora TERHADAP HERBISIDA REFLEX MENGGUNAKAN GEN PPX2L SEBAGAI PENANDA MOLEKULER Dwiati, Murni; Susanto, Agus Hery
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Fomesafen as an active substance of Reflex herbicide can inhibit PPOase, an enzyme playing important role in chlorophyl biosynthesis. Deletion of three bases at gene encoding PPOase, i.e. PPX2L, was reported as one of resistance mechanisms against PPOase inhibiting herbicides. Nevertheless, only a few studies on molecular characterization of Synedrella nodiflora resistance against Reflex were reported. Therefore, this study was aimed to (1) know the sequence of PPX2L gene isolated from resistant S. nodiflora against Reflex, (2) perform homology study of PPX2L gene from resistant S. nodiflora and various plant species in data base, and (3) know the sequence of PPX2L gene responsible to S. nodiflora resistance against Reflex. The PCR products of susceptible S. nodiflora showed three bands, in that of 500 bp is strongly assumed as PPX2L gene. Susceptible S. nodiflora is genetically different from susceptible A. tuberculatus, indicated by the absence of three base pairs at position 834, 835 and 836 in susceptible S. nodiflora, where in susceptible A. tuberculatus this position is occupied by CAG. Then, in both susceptible S. nodiflora and A. tuberculatus there is C at position 919 but T in resistant A. tuberculatus. At amino acid level this position is CCC (proline) in susceptible S. nodiflora, CTA (leucine) in susceptible A. tuberculatus and TTA (leucine) in resistant A. tuberculatus. Therefore, inspite of base alteration from C in susceptible A. tuberculatus to T in resistant A. tuberculatus, the amino acid formed remains constant, i.e. leucine. Significant difference is, however, observed in susceptible S. nodiflora because there is proline at the same posistion.
Sex identification in Nepenthes adrianii from Baturraden Botanical Garden: Genetic analysis using RAPD Markers Murni Dwiati; Zeihan Aziiza; Agus Hery Susanto
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.15750

Abstract

Nepenthes adrianii is one of pitcher plant species that grows endemically in Mount Slamet, Central Java. At present, it is one of pitcher plant collections of Baturraden Botanical Garden. Since N. adrianii is dioecious and both sexes are difficult to distinguish morphologically, early sex determination supporting its conservation at Baturraden Botanical Garden is needed. One approach can be performed with the use of RAPD molecular markers. Hence, this study aims to know whether differences in RAPD pattern between male and female N. adrianii exist or not and also to find out what the differences are. Genomic DNAs were extracted from leaves of 4 males, 2 females, and 2 sexually unidentified individuals. The extracted DNAs were then used to analyze DNA variation between male and female N. adrianii employing RAPD technique. As many as five oligonucleotide primers (OPA-15, OPK-16, OPP-15, OPP-08, and OPO-08) were used to amplify N. adrianii DNA. The results showed that one primer, i.e. OPK-16 (5’-GAGCGTCGAA-3’), produces a specific band of approximately 290 bp which is only found in female plants. It is assumed that this band is related to gene(s) controlling sex determination in N. adrianii. The RAPD marker can be used for the sex determination of young N. adrianii seedlings.
Intergeneric Hybridization between Phalaenopsis 2166 and Vanda ‘saint valentine’: Characterization of Parents Using ndhE cpDNA Partial Sequence Murni Dwiati; Agus Hery Susanto
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 3 (2021): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.65658

Abstract

An intergeneric cross between Phalaenopsis 2166 and Vanda ‘saint valentine’ has successfully produced protocorms that will be grown further to form seedlings. The present study aims to genetically characterize both parents by using ndhE partial gene as its sequence is shown polymorphic among five orchid genera of the subtribe Oncidiinae. The results reveal that the ndhE partial sequences of Phalaenopsis 2166 and Vanda ‘saint valentine’ are considerably homologous with those of Oncidium. However, alignment of ndhE partial sequences between both parents shows only 58% similarity, leading to the conclusion that a relatively high genetic difference between them may occur. 
Serapan Fosfor dan Pertumbuhan Kedelai(Glycine max) pada Tanah Ultisol dengan Pemberian Asam Humat Wahyuningsih Wahyuningsih; Elly Proklamasiningsih; Murni Dwiati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.911 KB) | DOI: 10.20884/1.mib.2016.33.2.345

Abstract

Ultisol merupakan salah satu jenis tanah asam yang memiliki kelarutan Al, Fe, dan Mn tinggi, serta kandungan P dan Moyang rendah. KelarutanAl dan Fe yangtinggiakan menjerap fosfat, sehingga ketersediaan dan serapan P bagi tanaman menjadi rendah. Salah satu upaya untuk mengatasi ketidaktersediaan P pada tanah Ultisol yaitu dengan penambahan asam humat. Asam humat merupakan makromolekul polielektrolit yang memiliki gugus fungsional seperti -COOH, -OH fenolat, maupun -OH alkoholat,sehingga asam humat memiliki kemampuan untuk membentuk kompleks dengan ion logam. Penelitian ini dilakukan dengan tujuan untuk: (1) mengetahui pengaruh asam humat terhadap serapan P dan pertumbuhan tanaman kedelai (2) menentukan konsentrasi asam humat yang paling baikdalam meningkatkan serapan P dan pertumbuhan tanaman kedelai. Metode penelitian yang digunakan adalah eksperimental dengan Rancangan Acak Kelompok (RAK). Pengelompokan didasarkan atas perbedaan intensitas cahaya yang ada di rumah kaca. Perlakuan berupa konsentrasi asam humat, dengan 5 konsentrasi yang berbeda yaitu 0 ppm, 400 ppm, 800 ppm, 1200 ppm dan 1600 ppm. Parameter yang diukur meliputi tinggi tanaman, luas daun, bobot basah, dan bobot kering, P tersedia serta P tanaman. Data yang diperoleh dianalisis menggunakananalisis ragam pada tingkat kepercayaan 95% dan 99%. Apabila hasil perlakuan berpengaruh nyata, maka dilanjutkan dengan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa asam humat berpengaruh terhadap serapan P dan pertumbuhan kedelai pada tanah Ultisol. Asam humat 1200 ppm merupakan perlakuan paling baik dalam meningkatkan ketersediaan P, sehingga mampu meningkatkanpertumbuhan kedelai pada tanah Ultisol.
Molecular Analysis of Synedrela Nodiflora (L.) Gaertn. Resistance Against Fomesafen using RAPD Markers Murni Dwiati; Agus Hery Susanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.290

Abstract

Both morphological and protein studies reveal that differences are observed between resistant Synedrella nodiflora against fomesafen and its susceptible wildtype. These are, however, more or less influenced by environmental factors, so that molecular analysis employing DNA markers is necessarily required. The methods involved total genomic DNA extraction using modified CTAB protocol following Doyle & Doyle (1990), RAPD marker amplification and visualization of RAPD markers prior to data analysis. Ten RAPD markers were used, but only seven of them showed polymorphism.  Calculation of genetic distance and variation was carried out employing PopGen software.Based on the RAPD markers used in this study, it can be concluded that genetic distance between susceptible and resistant S. nodiflora is higher than that within susceptible samples supporting our previous morphological and protein data, although genetic variation among susceptible individuals seems to be significantly high.
Pertumbuhan Stek Krisan (Chrysanthemum morifolium (L.) Ramat) pada Berbagai Media Kultur In Vitro Adven Kristianti; Kamsinah Kamsinah; Murni Dwiati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.2.207

Abstract

Chrysanthemum (Chrysanthemum morifolium (L.) Ramat is one of ornamental plant commodities with high aesthetic value. As a consequence of increasing demand, the availability of seeds in adequate number is needed with method in vitro. This study aims to find out the effect of various in vitro culture media on the growth of chrysanthemum explants and to determine alternative media other than MS for in vitro growth of chrysanthemum explants. Experimental method arranged in a Randomized Complete Block Design (RCBD) with four treatments and four blocks is applied. The treatments are Murashige and Skoog  (MS), Vacint and Went  (V&W), Knudson  and   modified media, while node position serves as block. Time of shoot emergence, shoot length, number of leaves on each shoot, and number of roots are measured. Data are analyzed using Analysis of Variance (F test) with confidence intervals of 95% and 99%. Least Significant Difference (LSD) test is applied when significant difference among variables is observed. Culture media shows significant effect on chrysanthemum explant in vitro growth. Knudson media is found as an alternative media besides MS sufficiently suitable to increase chrysanthemum explants in vitro growth.
Efek NAA dan BAP terhadap Pembentukan Tunas, Daun, dan Tinggi Tunas Stek Mikro Nepenthes ampullaria Jack. Heti Sartika Sari; Murni Dwiati; Iman Budisantosa
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 3 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.3.343

Abstract

This study aimed to know the interaction between NAA and BAP as well as to obtain the best combination of both treatments in promoting the growth of Nepenthes ampullariamicrocutting.An experiment arranged in a factorial Randomized Complete Block Design (RCBD) wasapplied. Stem segments were used as blocks, where block I was the first segment followed by the next two segments as block II and III respectively. Two factors, i.e. NAA concentrations (0, 5, 10, 15 µM) and BAP concentrations (0, 9, 18, 27 µM) were employed giving rise to 16 combination of treatments. Each treatment combination was replicated threetimes resulting in 48 experimental units. The parametersmeasured were date of shoot initiation, date of root initiation, shoot number, leaf number, root number, length of longest leafand shoot height. The results showed that interactionbetween NAA and BAP in promoting N. ampullariamicrocutting growth was observed.  Combination between NAA of 0 µM and BAP of 18 µMwas found to be the best in promoting N. ampullariamicrocutting growth.Meanwhile, combination between NAA 0 µM and BAP 27 µM was recommended to promote shoot number of N. ampullaria.
Penambahan Ekstrak Alga Sargassum duplicatum Bory pada Medium Kultur In Vitro terhadap Pertumbuhan seedling Anggrek Vanda tricolor Lindl. Eko Adiguna; Murni Dwiati; Kamsinah Kamsinah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2018.35.1.595

Abstract

Vanda tricolor is an orchid species that has many advantages, some of which are due to its beautiful and various flowers of unique shape and size. Propagation of V. tricolor can be performed by in vitro culture.In vitro culture technique needs appropriate medium to support orchid growth. An algae species, Sargassum duplicatum, can be added into the medium, since it contains growth regulating substances, such as auxin and gibberellin, which are useful for seedling growth. This study aims to know the effect of S. duplicatum application into in vitro culture medium of V. tricolor on the seedling growth and to know the best application of the algaein increasing seedling growth. An experimental method was arranged in a Completely Randomized Design (CRD). The treatments were S. duplicatum weight of 0, 12, 24, 36, 48 and 60 g/L replicated three times respectively. The variable examined was seedling growth with parameters comprising day of new leaf emergence, leaf number, leaf length, day of new root emergence, root number, root length and plant height. Data were analyzed using ANOVA or F test with confidence level of 95% and 99%. LSD was carried out when F test showed significant difference. The results reveal that application of S. duplicatum extract has significant effect on the growth of V. tricolor seedling. The best application is of 36 g/L algae weight.
VARIATION IN SUGAR CONTENT AND DISTRIBUTION IN SYZYGIUM SAMARANGENSE FRUITS Pudji Widodo; Elly Proklamasiningsih; Murni Dwiati; Agus Hery Susanto
Floribunda Vol. 7 No. 3 (2023): Floribunda Oktober 2023
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v7i3.2023.422

Abstract

Jambu semarang (Syzygium samarangense) terdiri dari banyak kultivar dengan kadar gula yang berbeda-beda. Pada setiap buah, kandungan gulanya tidak merata. Tingkat kemanisan buah seringkali menjadi penentu nilai ekonomi dari jambu semarang. Tujuan penelitian ini adalah 1) untuk mengetahui sebaran gula pada berbagai kultivar jambu semarang, 2) mengetahui sebaran kadar gula di dalam buahnya, 3) mengetahui pengaruh rerata hari hujan per bulan terhadap kandungan gula buah.  Metode yang digunakan dalam penelitian ini adalah survei dengan purposive sampling, observasi laboratorium dengan mengukur kadar gula dengan refraktometer, mengkorelasikan faktor lingkungan dengan kandungan gula buah. Hasil penelitian ini menunjukkan bahwa buah dengan kadar gula tertinggi terdapat pada kultivar Syzygium samarangense 'Sukaluyu' (maks 16,5 ◦Brix), S. samarangense 'Madu' (maks 14 ◦Brix), S. samarangense 'Kesuma Merah’ (max 10.5 ◦Brix), S. samarangense 'Citra' (maks 8.5◦Brix) yang cocok untuk buah-buahan segar. Sedangkan yang kadar gulanya rendah seperti S. samarangense 'Bajang Leang’ (4.5 ◦Brix), S. samarangense 'Kuning’ (4 ◦Brix) lebih cocok untuk lutis. Pada buah S. samarangense, gula tertinggi biasanya terakumulasi di bagian ujung buah, dan gula terendah berada di pangkal buah.  Kadar gula berkorelasi negatif dengan rerata hari hujan per bulan.  Makin sedikit hari hujan, buah semakin manis.  
Co-Authors Adven Kristianti Agus Hery Susanto AGUS HERY SUSANTO Agus Hery Susanto Eko Adiguna Elly Proklamasiningsih Elly Proklamasiningsih Heti Sartika Sari Iman Budisantosa Kamsinah Kamsinah Kamsinah Kamsinah Pudji Widodo Wahyuningsih Wahyuningsih Zeihan Aziiza