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All Journal INDONESIAN JOURNAL OF PHARMACY
Djatmiko M.
P.T. Phapros Tbk. Semarang
Medicine & Pharmacology

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STANDARDIZATION OF CELERY LEAVES PREPARATION (Apium graveolens L.) BY THIN LAYER CHROMATOGRAPHIC – DENSITOMETRY USING APIGENIN AS PARAMETER Djatmiko M.; Pramono S.
Indonesian Journal of Pharmacy Vol 12 No 2, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.399 KB) | DOI: 10.14499/indonesianjpharm0iss0pp59-64

Abstract

In order to apply a standardization method on herbal medicines or pharmaceuticals, a thin layer chromatographic-densitometry was used to determine apigenin as the active constituent of celery extract formulated in a capsule dosage form. Silica gel GF 254 and chloroform – methanol – water (70 – 30 – 6,5 v/v) were used as stationary and mobile phases, respectively. The areas of apigenin were evaluated at 340 mm using a TLC-Scanner.The limit of detetion was 3 g and the accuracy was 89,6% after first extraction prosedure applied on celery capsules. The apigenin content was 0,017 %.Key Words : Apium graveolens L., apigenin, TLC – densitometry.
Co-Authors Pramono S.