STANDARDIZATION OF CELERY LEAVES PREPARATION (Apium graveolens L.) BY THIN LAYER CHROMATOGRAPHIC – DENSITOMETRY USING APIGENIN AS PARAMETER

Djatmiko M. (P.T. Phapros Tbk. Semarang)
Pramono S. (Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta)

INDONESIAN JOURNAL OF PHARMACY
Vol 12 No 2, 2001

In order to apply a standardization method on herbal medicines or pharmaceuticals, a thin layer chromatographic-densitometry was used to determine apigenin as the active constituent of celery extract formulated in a capsule dosage form. Silica gel GF 254 and chloroform – methanol – water (70 – 30 – 6,5 v/v) were used as stationary and mobile phases, respectively. The areas of apigenin were evaluated at 340 mm using a TLC-Scanner.The limit of detetion was 3 g and the accuracy was 89,6% after first extraction prosedure applied on celery capsules. The apigenin content was 0,017 %.Key Words : Apium graveolens L., apigenin, TLC – densitometry.