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All Journal Jurnal Penelitian Pascapanen Pertanian Journal of Fisheries & Marine
Pujoyuwono Martosuyono
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Stabilitas Panas Enzim PDC Dari Bakteri Termofil Penghasil Etanol Pujoyuwono Martosuyono; Peter L Rogers
Jurnal Penelitian Pascapanen Pertanian Vol 2, No 2 (2005): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v2n2.2005.49-55

Abstract

Penggunaan bakteri termofil telah menarik banyak pihak, terutama dalam sistem produksi bahan bakar alternatif seperti bioetanol. Keuntungan pemakaian bioetanol adalah dapat menurunkan tingkat polusi dan pemanasan global karena hasil pembakaran etanol tidak memberikan tambahan netto CO2 pada lingkungan. satu isolat bakteri termofilik unggul telah diisolasi dari sampel kompos yang menunjukkan kemampuan untuk memproduksi etanol, Hasil identifikasi dengan sekuensing 16S rRNA menunjukkan isolat ini diklasifikasikan sebagai Geobacillus thermoleovorans, bakteri gram positif berbentuk batang yang mampu tumbuh pada kisaran substrat yang luas, dengan suhu optimum pertumbuhan 600C dan bersifat fakultatif anaerob. Isolat tersebut juga menunjukkan aktivitas enzim pyruvate decarboxylase (PDC), enzim kunci dalam produksi etanol, Hasil pengukuran aktivitas pada suhu 25, 40 dan 600C yang menunjukkan stabilitas yang tinggi terhadap pemanasan dengan meningkatnya aktivitasnya setelah dilakukan pemanasan pada suhu 600C  dibandingkan dengan PDC dari Zymomonas mobilis yang kehilangan aktivitasnya secara total. Komponen lain dalam asay enzim sensitif terhadap panas sehingga pengukuran aktivitas hanya bisa dilakukan pada suhu 250CThermal stability of PDC enzyme from thermophilic bacteria producing ethanolThere has been considerable interest in the use of thermophiles for the production of fuel and bulk chemicals such as alcohol and acids. The advantage of ethanol utilization as a fuel is the capability to reduce the pollution level and global warming since it recycles atmospheric carbon from renewable biomass such as cellulose, hemicelluose and starch, thus 'closing the carbon balance'. One isolat of thermophilic bacteria with the capability to produce ethanol had been isolated from compost sample. Identification by 16S rRNA sequencing has revealed that the isolat is classified as Geobacillus thermoleovorans, a gram positive bacteria, rod shape, grow on wide variety of substrates with optimum growing temperature of 600C, and facultative anaerobe. The cell extract from isolate 0·2124 shows the presence of pyruvate decarboxylase; the key enzyme in ethanol production system. Activity measurement of POC at 25, 40 and 600C demonstrate the thermostable character of the enzyme. Total activity of pre-incubated cell extract at 600C was higher than other temperature while the activity of the counterpart enzyme of Zymomonas mobilis was totally lost at that temperature. The other components of enzyme assay were thermal sensitive, consequently the activity measurement was carried out at 250C.
Optimization of Effervescent Tablets in Sensory Acceptance from the Active Ingredients of Fish Protein Hydrolysate and Microalgae Chlorellae sp. powder Yuniarti, Tatty; Nanda Anggiani Putri; M.Si, Niken Dharmayanti; Mugi Mulyono; Sri Sugiwati; Pujoyuwono Martosuyono; Ita Junita Puspa Dewi; Aghitia Maulani; Taufik Hidayat
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 1 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.56019

Abstract

Graphical Abstract Highlight Research 1. FPH and Chlorella sp. microalgae can be used as effervescent tablet ingredients received by panelists. 2. The mixture design method can determine the formula of effervescent tablets made from a combination of FPH and microalgae 3. The best percentage of FPH and microalgae in effervescent tablets was 5.873% and 9.127%.     Abstract Effervescent tablets are a promising delivery system for functional food ingredients, offering convenience and enhanced consumer appeal. This study aimed to optimize effervescent tablet formulations incorporating Fish Protein Hydrolysate (FPH) and Chlorella sp. powder using the d-optimal mixture design method. Thirteen formulations were evaluated for sensory attributes (color, aroma, taste, aftertaste, hedonic scores) and chemical properties (protein, lipid, water content). The optimal formulation, consisting of 5.873% FPH and 9.127% Chlorella sp. powder, significantly improved sensory acceptance (p < 0.05), validated by a robust statistical model (R2 = 0.93). Proximate analysis confirmed the nutritional value, with protein contents of 19.60% and 47.68% in FPH and Chlorella sp., respectively, along with flavonoids (2.69%) in Chlorella sp.. These results demonstrate the successful integration of bioactive compounds into effervescent formulations, addressing consumer demand for sustainable and health-promoting products. This research provides a framework for developing innovative functional food products, contributing to the global advancement of nutraceuticals and sustainable food solutions. Future studies should focus on long-term stability, bioavailability, and consumer acceptability to enhance the commercialization potential of these effervescent tablets.
Co-Authors Ita Junita Puspa Dewi M.Si, Niken Dharmayanti Maulani, Aghitia Mulyono, Mugi Nanda Anggiani Putri Peter L Rogers Sri Sugiwati Taufik Hidayat Yuniarti, Tatty