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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Berkala Kedokteran
Edi Widjajanto
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Secretory Leukocyte Protease Inhibitor (SLPI) Decreased the Celluler Expression of NF-Kβ and IL-1β on Wound Macrophages of Rattus novergicus Post Tooth Extraction Agustine Hanafi Putri; Abdur Razaq Komaruzzaman; Putri Noerpuspita; Delfi Fitriyani; Nur Permatasari; Edi Widjajanto
Journal of Tropical Life Science Vol. 6 No. 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.05

Abstract

Prevalence of tooth extraction or dental surgery was 48.5% of all dental care in Indonesia. Tooth extraction carries potential health risks and side effects such as pain, swelling, trismus and dysfunction of the oral cavity during recovery. Secretory leukocyte protease inhibitor (SLPI) is one of innate immunity proteins that can inhibit the activation of macrophages. We are expecting the provision of SLPI can decrease excessive inflammatory response in healing after tooth extraction. This study was to investigate the administration of SLPI on cellular expression of NF-Kβ and IL-1β on wound macrophages of Rattus novergicus post tooth extraction. The research design is in vivo experimental study. In total, 20 rats were randomly divided into four groups (each group n=5) and underwent tooth extraction on left incisor teeth of mandible. One of the groups did not receive SLPI administration (control group) and the socket was stitched after tooth extraction. Meanwhile, the remaining three groups (experimental groups) were given SLPI administration after tooth extraction with three different doses (0.1 µM, 0.5 µM and 2.5 µM, respectively). After SLPI administration, the socket of experimental groups was stitched. The effects of SLPI administration were evaluated by counting at the percentage of NF-Kβ translocation and the expression of expression of IL-1 in macrophages cells of the rat socket using immunohistochemistry analysis. The cellular expression of NF-Kβ and IL-1β were significantly decreased (p < 0.05) on groups with SLPI may decrease cellular expression of NF-Kβ and IL-1β on wound macrophages cells of rats post tooth extraction in a dosedependent manner.
Levels of 25(OH)D3, IL-2, and C-peptide in Children with Type 1 Diabetes Mellitus (T1DM) Receiving Vitamin D3 Supplementation Tjahyo Suryanto; Harjoedi Adji Tjahjono; Edi Widjajanto
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.06

Abstract

Type 1 Diabetes Mellitus (T1DM) has become a health problem in many countries. T1DM is the consequence of autoimmune destruction process of β cells. There was relationship between vitamin D deficiency with T1DM. The destruction process was caused by an imbalance of pro-inflammatory and anti-inflammatory cytokines. One of the pro-inflammatory cytokines is IL-2. C-peptide examination to see the function of beta cells due to destruction of pancreatic beta cell. Administration of vitamin D3 supplementation still cause controversy and give varying results. This randomized clinical trial was conducted to determine the levels of 25(OH)D3, IL-2, and C-peptide in people with T1DM who received vitamin D3 supplementation. The subjects were 26 children with T1DM, divided into K1 group (received vitamin D3 supplementation) and K2 group (received placebo). The results showed higher levels of 25(OH)D3 in the K1 group and statistically found a significant difference (p = 0.00). Higher levels of IL-2 and lower C-peptide were obtained in the K1 group and no statistically significant differences were found (p = 0.76 and p= 0.26). The insignificant relationship and the negative correlation were found between 25(OH)D3 and IL-2 (p = 0.71; r = - 0.12), 25(OH)D3 and C-peptide (p = 0.59; r = -0.16), also levels of IL-2 and C-peptide (p = 0.13; r = -0.44) in children with type 1 diabetes who received vitamin D3 supplementation. From this study can be concluded that administration vitamin D3 supplementation in patients with T1DM can increase levels 25(OH)D3 significantly. This increase has not significantly lowered levels of IL-2 and increased levels of C-peptide. However, there was an absolute decrease in the rate of slower C-peptide in the supplemented group than in the placebo group.
Co-Authors Abdur Razaq Komaruzzaman Agustine Hanafi Putri Delfi Fitriyani Harjoedi Adji Tjahjono Karyono Mintaroem Moch Sasmito Djati Mulyohadi Ali Nelwan, Ester Jeini Nur Permatasari Poerwosusanta, Hery Putri Noerpuspita Sri Andarini Tjahyo Suryanto Zairin Noor