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All Journal Indonesia Medicus Veterinus Majalah Obat Tradisional Journal of Science and Technology
Dini Maharani
Udayana University
Computer Science & IT Health Professions Mathematics Medicine & Pharmacology Public Health Veterinary

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Karakteristik Lokus Mikrosatelit D10s1432 pada Populasi Monyet Ekor Panjang Di Taman Nasional Alas Purwo Banyuwangi Maharani, Dini; Soma, I Gede; Wandia, I Nengah
Indonesia Medicus Veterinus Vol 3 (3) 2014
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.624 KB)

Abstract

Karakterisasi lokus mikrosatelit berbagai marka molekul untuk mengungkapkan variasi genetik populasi perlu dilakukan. Penelitian ini bertujuan untuk mengungkapkan karakteristik lokus mikrosatelit D10S1432 yang meliputi jumlah alel, frekuensi alel, dan heterozigositas pada populasi monyet ekor panjang di Taman Nasional Alas Purwo. Sejumlah 14 sampel darah dari vena femoralis dikoleksi dari populasi monyet ekor panjang di Taman Nasional Alas Purwo sebagai sumber DNA. Ekstraksi DNA total menggunakan QIAamp DNA Blood Kits dari Qiagen. Selanjutnya lokus mikrosatelit D10S1432 diamplifikasi melalui Polymerase Chain Reaction (PCR) sebanyak 30 siklus dengan suhu annealing 57°C. Variasi alel mikrosatelit dipisahkan dengan elektroforesis pada gel poliakrilamid 7% dan dimunculkan dengan pewarnaan perak. Penelitian mengidentifikasi empat jenis alel pada lokus D10S1432 dengan panjang berkisar antara 164 bp sampai 170 bp. Frekuensi alel bervariasi, yaitu alel 164 (0,10); 166 (0,32); 168 (0,29); dan 170 (0,29), serta nilai heterozigositas lokus sebesar 0,75. Dari hasil penelitian dapat disimpulkan bahwa lokus mikrosatelit D10S1432 pada populasi monyet ekor panjang di Taman Nasional Alas Purwo bersifat polimorfik, dan marka yang baik dapat mengungkapkan variasi genetik populasi.
Pengembangan Video Pembelajaran Untuk Pengenalan Kosa Kata Bahasa Arab Anak Usia Dini Berbasis Aplikasi Inshot Maharani, Dini; Warmansyah, Jhoni
JOSTECH: Journal of Science and Technology Vol 2, No 1: Maret 2022
Publisher : UIN Imam Bonjol Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15548/jostech.v2i1.3806

Abstract

This study aims to develop an inshot application-based learning video for early childhood Arabic vocabulary introduction (AUD). The type of research used is research and development (R&D) research using a 4D development model, but in this study it is only limited to 3 stages, namely the definition stage, the design stage, and the develop stage. This research was conducted at TKIT Qurrata A'yun Batusangkar. Based on the validity and practicality test of learning video media products using the inshot application for the introduction of Arabic vocabulary in children, it appears that this media is very valid and very practical. . This is indicated by the results of the validity with a percentage of 83.75% with very valid criteria and the results of the test results with a percentage of 97.5% with very practical criteria.
Anti-aging Effect of Black Garlic Through Anti-senescence, Gelatinase Inhibition Mechanism, and Formulation of NLC Serum Effendi, Fatiha Citra; Nabila, Klarissa; Maharani, Dini; Widayanti, Wasita Rachma; Jauhari, Fahmi Ihsanuddin; Meiyanto, Edy; Lukitaningsih, Endang
Majalah Obat Tradisional Vol 30, No 1 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.90878

Abstract

Aging associated with cellular senescence was responsible for the degradation of collagen and elastin by activation of matrix metalloproteinases (MMPs) that produced wrinkles. Black garlic is known to have an anti-aging potency on premature aging. This study aims to reveal the anti-aging potency of black garlic through anti-senescence and gelatinase inhibition mechanisms and its formulation of Nanostructured Lipid Carrier Serum. Black Garlic Extract (BGE)  was macerated with ethanol 50% then heated with low temperature at 50°C. The extract obtained was profiled with Thin Layer Chromatography and antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The cytotoxic effect of BGE was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on Vero cells. Anti-senescence effect of BGE was conducted by SA-β-Gal assay. The inhibition of gelatinase activities was predicted by molecular docking using MOE2010 software. The preparation of Nanostructured Lipid Carrier (NLC) is done by High Shear Homogenization method, then the best formula continued to make NLC-BGE Serum. The BGE contained S-allyl cysteine as a major organosulfur compound. BGE showed non-toxic to Vero cells with IC50 >500 μg/mL. Furthermore, 50 μg/mL of BGE showed inhibits doxorubicin-induced senescence in Vero cells. BGE also appeared to have good affinity on inhibitory domains of MMP-1 (∆G -7,754 kcal/mol) and MMP-2 (∆G -9,130 kcal/mol). NLC-BGE serum formula has met nanoparticles criteria and showed good stability. Based on this study, BGE revealed anti-senescence and gelatinase inhibition that is considered to have high anti-aging properties and can be applied in the NLC-BGE serum formula.
Co-Authors Edy Meiyanto Effendi, Fatiha Citra Endang Lukitaningsih I Gede Soma i Nengah Wandia Jauhari, Fahmi Ihsanuddin Jhoni Warmansyah Nabila, Klarissa Widayanti, Wasita Rachma