The research was conducted to investigate the characteristic and performance of Limura grade and Madura cows. The research was conducted in Sumenep and Pamekasan Regencies East Java Province within the period of May until August 2008. The method being used was an interview method on the farmer as respondents, continued by observing the exterior characteristics and the cattle measurements. The data of exterior characteristic were analyzed bydescriptive patterns. The data of vital measurements of the body were analyzed by independent sample t-test. The result showed that the general characteristics Limura crossbred cows had a dominant color of a reddish brown, with thebuttock color was a reddish brown. The general characteristics Madura cows had a dominant color of a light red, with the buttock color was a white smear. The vital characteristics of Limura crossbred cows was higher than Madura cows,at the 2 to 4 years old group cows the result showed that girth of chest, height at hip of Limura crossbred cows were 172.63±2.33 cm; 125.07±1.40 cm. while those of Madura cows were 140.81±3.01 cm; 114.77±1.29 cm. At older than 4years old group cows the result showed that heart girth, height at hip of Limura crossbred cows were 172.85±4.12 cm; 126.15±1.84 cm. while those of Madura cows were 157.57±2.55 cm; 118.54±1.53 cm. The performance of Limuracrossbred cows and Madura cows related to calving interval were 15.90±0.47 vs 14.39±0.23 months. The result showed that the general characteristics Limura crossbred cows and Madura cows had a different color at the dominant color ofbody and the buttock color, and Limura crossbred cows had a vital characteristics size higher than Madura cows, but the Madura’s reproductive performance showed better than Limura crossbred cows.(Key words: Characteristics, Performance, Limura crossbred cows, Madura cows)